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Q3IK75

- MIAB_PSEHT

UniProt

Q3IK75 - MIAB_PSEHT

Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

    Catalytic activityi

    N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi158 – 1581Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi162 – 1621Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi165 – 1651Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. transferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPHAL326442:GJIU-1053-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
    Alternative name(s):
    (Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
    tRNA-i(6)A37 methylthiotransferaseUniRule annotation
    Gene namesi
    Name:miaBUniRule annotation
    Ordered Locus Names:PSHAa1046
    OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
    Taxonomic identifieri326442 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
    ProteomesiUP000006843: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374461Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi326442.PSHAa1046.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3IK75.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 120118MTTase N-terminalUniRule annotationAdd
    BLAST
    Domaini379 – 44264TRAMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
    Contains 1 MTTase N-terminal domain.UniRule annotation
    Contains 1 TRAM domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0621.
    HOGENOMiHOG000224767.
    KOiK06168.
    OrthoDBiEOG6P5ZD8.

    Family and domain databases

    Gene3Di3.80.30.20. 1 hit.
    HAMAPiMF_01864. tRNA_metthiotr_MiaB.
    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view]
    PANTHERiPTHR11918. PTHR11918. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
    PROSITEiPS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3IK75-1 [UniParc]FASTAAdd to Basket

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    MSKKLHIKTW GCQMNEYDSQ KMAELLDATN GYQLTDDATD ADVILLNTCS    50
    IREKAQEKVF HQLGRWKLLK DDKPDLIIGV GGCVASQEGD SIRQRAPFVD 100
    VIFGPQTLHR LPEMIKQVQG DKGSSVVDIS FPEIEKFDRL PEPKADGPSA 150
    FVSIMEGCSK YCTFCVVPYT RGEEVSRPVD DVLLEIAQLA EQSVREVNLL 200
    GQNVNAYRGD THDGEICYFS DLIRLIAAID GIDRIRYTTS HPVEFTQDIV 250
    DVYADVPELV DHLHLPVQSG SDRILNLMKR GHTAIEYKST IRKLRKIRPN 300
    LSMSSDFIIG FPGETQDDFE ATMKLISDVG FDMSFSFIYS ARPGTPAADL 350
    PDDVTEQEKK ERLYLLQNRI TQMAQQISRQ MFDTEQRILV EGPSKKNPME 400
    LRGRTENNRV VNFVGPHTVI GQFVDVRITE ALPNSLRGDL IRTESEMNLR 450
    REIAPSAILT KAASLEPKPD TINEIGVATF VP 482
    Length:482
    Mass (Da):54,180
    Last modified:May 5, 2009 - v2
    Checksum:iB32E31AD6C40C11F
    GO

    Sequence cautioni

    The sequence CAI86124.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954246 Genomic DNA. Translation: CAI86124.1. Different initiation.
    RefSeqiYP_339567.1. NC_007481.1.

    Genome annotation databases

    EnsemblBacteriaiCAI86124; CAI86124; PSHAa1046.
    GeneIDi3710200.
    KEGGipha:PSHAa1046.
    PATRICi32295619. VBIPseHal105694_1006.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954246 Genomic DNA. Translation: CAI86124.1 . Different initiation.
    RefSeqi YP_339567.1. NC_007481.1.

    3D structure databases

    ProteinModelPortali Q3IK75.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 326442.PSHAa1046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI86124 ; CAI86124 ; PSHAa1046 .
    GeneIDi 3710200.
    KEGGi pha:PSHAa1046.
    PATRICi 32295619. VBIPseHal105694_1006.

    Phylogenomic databases

    eggNOGi COG0621.
    HOGENOMi HOG000224767.
    KOi K06168.
    OrthoDBi EOG6P5ZD8.

    Enzyme and pathway databases

    BioCyci PHAL326442:GJIU-1053-MONOMER.

    Family and domain databases

    Gene3Di 3.80.30.20. 1 hit.
    HAMAPi MF_01864. tRNA_metthiotr_MiaB.
    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view ]
    PANTHERi PTHR11918. PTHR11918. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
    PROSITEi PS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TAC 125.

    Entry informationi

    Entry nameiMIAB_PSEHT
    AccessioniPrimary (citable) accession number: Q3IK75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3