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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi158Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi162Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi165Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPHAL326442:G1GI4-1026-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:PSHAa1046
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
Proteomesi
  • UP000006843 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003744611 – 482tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST482

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi326442.PSHAa1046

Structurei

3D structure databases

ProteinModelPortaliQ3IK75
SMRiQ3IK75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 120MTTase N-terminalUniRule annotationAdd BLAST118
Domaini379 – 442TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0621 LUCA
HOGENOMiHOG000224767
KOiK06168
OrthoDBiPOG091H00LG

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q3IK75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKLHIKTW GCQMNEYDSQ KMAELLDATN GYQLTDDATD ADVILLNTCS
60 70 80 90 100
IREKAQEKVF HQLGRWKLLK DDKPDLIIGV GGCVASQEGD SIRQRAPFVD
110 120 130 140 150
VIFGPQTLHR LPEMIKQVQG DKGSSVVDIS FPEIEKFDRL PEPKADGPSA
160 170 180 190 200
FVSIMEGCSK YCTFCVVPYT RGEEVSRPVD DVLLEIAQLA EQSVREVNLL
210 220 230 240 250
GQNVNAYRGD THDGEICYFS DLIRLIAAID GIDRIRYTTS HPVEFTQDIV
260 270 280 290 300
DVYADVPELV DHLHLPVQSG SDRILNLMKR GHTAIEYKST IRKLRKIRPN
310 320 330 340 350
LSMSSDFIIG FPGETQDDFE ATMKLISDVG FDMSFSFIYS ARPGTPAADL
360 370 380 390 400
PDDVTEQEKK ERLYLLQNRI TQMAQQISRQ MFDTEQRILV EGPSKKNPME
410 420 430 440 450
LRGRTENNRV VNFVGPHTVI GQFVDVRITE ALPNSLRGDL IRTESEMNLR
460 470 480
REIAPSAILT KAASLEPKPD TINEIGVATF VP
Length:482
Mass (Da):54,180
Last modified:May 5, 2009 - v2
Checksum:iB32E31AD6C40C11F
GO

Sequence cautioni

The sequence CAI86124 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA Translation: CAI86124.1 Different initiation.
RefSeqiWP_041454380.1, NC_007481.1

Genome annotation databases

EnsemblBacteriaiCAI86124; CAI86124; PSHAa1046
KEGGipha:PSHAa1046
PATRICifig|326442.8.peg.1006

Similar proteinsi

Entry informationi

Entry nameiMIAB_PSEHT
AccessioniPrimary (citable) accession number: Q3IK75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: March 28, 2018
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health