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Q3IK75

- MIAB_PSEHT

UniProt

Q3IK75 - MIAB_PSEHT

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Protein
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Gene
miaB, PSHAa1046
Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarity
Metal bindingi158 – 1581Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi162 – 1621Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi165 – 1651Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-1053-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (EC:2.8.4.3)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene namesi
Name:miaB
Ordered Locus Names:PSHAa1046
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843: Chromosome I

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation
PRO_0000374461Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi326442.PSHAa1046.

Structurei

3D structure databases

ProteinModelPortaliQ3IK75.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 120118MTTase N-terminal
Add
BLAST
Domaini379 – 44264TRAM
Add
BLAST

Sequence similaritiesi

Contains 1 TRAM domain.

Phylogenomic databases

eggNOGiCOG0621.
HOGENOMiHOG000224767.
KOiK06168.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IK75-1 [UniParc]FASTAAdd to Basket

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MSKKLHIKTW GCQMNEYDSQ KMAELLDATN GYQLTDDATD ADVILLNTCS    50
IREKAQEKVF HQLGRWKLLK DDKPDLIIGV GGCVASQEGD SIRQRAPFVD 100
VIFGPQTLHR LPEMIKQVQG DKGSSVVDIS FPEIEKFDRL PEPKADGPSA 150
FVSIMEGCSK YCTFCVVPYT RGEEVSRPVD DVLLEIAQLA EQSVREVNLL 200
GQNVNAYRGD THDGEICYFS DLIRLIAAID GIDRIRYTTS HPVEFTQDIV 250
DVYADVPELV DHLHLPVQSG SDRILNLMKR GHTAIEYKST IRKLRKIRPN 300
LSMSSDFIIG FPGETQDDFE ATMKLISDVG FDMSFSFIYS ARPGTPAADL 350
PDDVTEQEKK ERLYLLQNRI TQMAQQISRQ MFDTEQRILV EGPSKKNPME 400
LRGRTENNRV VNFVGPHTVI GQFVDVRITE ALPNSLRGDL IRTESEMNLR 450
REIAPSAILT KAASLEPKPD TINEIGVATF VP 482
Length:482
Mass (Da):54,180
Last modified:May 5, 2009 - v2
Checksum:iB32E31AD6C40C11F
GO

Sequence cautioni

The sequence CAI86124.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI86124.1. Different initiation.
RefSeqiYP_339567.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI86124; CAI86124; PSHAa1046.
GeneIDi3710200.
KEGGipha:PSHAa1046.
PATRICi32295619. VBIPseHal105694_1006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI86124.1 . Different initiation.
RefSeqi YP_339567.1. NC_007481.1.

3D structure databases

ProteinModelPortali Q3IK75.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326442.PSHAa1046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI86124 ; CAI86124 ; PSHAa1046 .
GeneIDi 3710200.
KEGGi pha:PSHAa1046.
PATRICi 32295619. VBIPseHal105694_1006.

Phylogenomic databases

eggNOGi COG0621.
HOGENOMi HOG000224767.
KOi K06168.
OrthoDBi EOG6P5ZD8.

Enzyme and pathway databases

BioCyci PHAL326442:GJIU-1053-MONOMER.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
HAMAPi MF_01864. tRNA_metthiotr_MiaB.
InterProi IPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view ]
PANTHERi PTHR11918. PTHR11918. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
PROSITEi PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiMIAB_PSEHT
AccessioniPrimary (citable) accession number: Q3IK75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: May 14, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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