Q3IJH1 (ILVD1_PSEHT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 1
Short name=DAD 1
EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	PSHAa2769

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000225408
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3IJH1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 39416930A266EFAD	FASTA	618	65,833
	10 20 30 40 50 60 MAKLRSATTT EGRKRAGARA LWRATGMTDS DFGKPIIAVV NSFTQFVPGH VHLNQLSELM 70 80 90 100 110 120 GETITAAGGV PKEFNTIAID DGIAMGHGGM LYSLPSRDLI ADSVEYMVNG HCADAMICIS 130 140 150 160 170 180 NCDKITPGMM LAALRLNIPV IFVSGGPMEA GKTKLANIDI KLDLVDAMVK GADESVSDAD 190 200 210 220 230 240 SDQVERSACP TCGSCSGMFT ANSMNCLLEA IGLALPGNGT TLATHKDRKQ LYVEAGARIV 250 260 270 280 290 300 DLCREYYQKD NQEVLPRAIA NKTAFMNAMV VDIAMGGSSN TVLHLLAAAQ EAGVDFDMSH 310 320 330 340 350 360 IDALSRKTPF LCKVAPATPE YHIEDVHRAG GMMAIVRELG KAGLVDMSVN HVAGSTMAEL 370 380 390 400 410 420 VKKWDATDPT NEAARKFYRA GPAGIRTTKA MSQECRWDEG DNDREHGCIR SVEHAFRQDG 430 440 450 460 470 480 GLAVLSGNLA VDGCVVKSAG VVDDMLHFEG TAVVYESQDD SVEGILNDEV KAGDVVVIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTSYLK SKGLAEKCAL ITDGRFSGGT SGLSIGHVSP EAASGGAIAY 550 560 570 580 590 600 VENGDKIIID IATREITLAL SDAELEARKQ KQLARGKQAY KPLDRQRYVS SALKAYALLA 610 TSADKGAVRD LAKLEELS

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TAC 125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954246 Genomic DNA. Translation: CAI87817.1.
RefSeq	YP_341259.1. NC_007481.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IJH1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3710093.
GenomeReviews	Gene locus PSHAa2769 in contig CR954246_GR.
KEGG	pha:PSHAa2769.
NMPDR	fig|326442.4.peg.2340.
PATRIC	32299121. VBIPseHal105694_2678.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q3IJH1.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA2769-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_PSEHT

Accession

Primary (citable) accession number: Q3IJH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program