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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei625 – 6251ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-1038-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PSHAa1031
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Leucine--tRNA ligasePRO_1000009400Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi326442.PSHAa1031.

Structurei

3D structure databases

ProteinModelPortaliQ3IJ93.
SMRiQ3IJ93. Positions 228-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IJ93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYNPQKI ESKIQRYWEE NKVFKVTEDE SKEKYYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VVSRFQRLKG KNVMQPMGWD AFGLPAENAA IKNKTAPAKW
110 120 130 140 150
TYENIDYMRT QLKQLGFGYD WDREIATCHP EYYKWEQWFF TKLYEKGLVY
160 170 180 190 200
KKMSTVNWDP VDQTVLANEQ VIDGRGWRSG AVVEQKEIPQ WFIKITDYAQ
210 220 230 240 250
ELLDDLDKLE DWPEQVKTMQ RNWIGRSEGC DIEFKRTDNN ETFSVYTTRP
260 270 280 290 300
DTFMGVTYVA VAAGHPIAKE AAKNSDSVAL FVEECKNNKV SEADMATMEK
310 320 330 340 350
KGIATGFFAT HPLTGEQVPI WIANFVLMHY GSGAVMAVPA HDQRDFEFAT
360 370 380 390 400
AYGLDIKQVI APVTGSDLEV NLNKEAFTDK GVLVNSGEFD GLDFDAAFNA
410 420 430 440 450
IADKLEALGV GERKVNFRLR DWGVSRQRYW GSPIPMLSDE DGNELAATDD
460 470 480 490 500
MLPVRLPEDV VMNGVTSPIK ADPNWAKTTM NGAPAFHETD TFDTFMESSW
510 520 530 540 550
YYARYCSPRH DEGMLDPAAA NYWLPVNQYI GGIEHAILHL LYSRFFHKLL
560 570 580 590 600
RDFGLVDSDE PFDRLLCQGM VLAETFYRKD EKGADIWISP SDVNTETDDK
610 620 630 640 650
GRVTKAWHKV DGNPVFSSGM SKMSKSKNNG IDPQHVIAQY GADTVRLFMM
660 670 680 690 700
FTAPPEQTLE WSDAGVEGAH RFLKRVWKYA VDVKTVGFQP LNKTALNNEQ
710 720 730 740 750
KVLRRDLHKA IAKVSDDVER RQTFNTAIAA IMELSNKLLK APLNSKQDVA
760 770 780 790 800
IANEALEAML IMLAPITPHL SHQLWQELGK EGDIVNASWP EVDQSALVED
810 820 830 840 850
EKLIIIQVNG KLRAKLTVAA DATKEQVEAL AFEQASVTKY TDGVTVRKVI
860
YVPGKLLNVV AN
Length:862
Mass (Da):97,721
Last modified:November 8, 2005 - v1
Checksum:i323B8C27F25615BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA. Translation: CAI86109.1.
RefSeqiWP_011327720.1. NC_007481.1.
YP_339552.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI86109; CAI86109; PSHAa1031.
GeneIDi3710242.
KEGGipha:PSHAa1031.
PATRICi32295589. VBIPseHal105694_0991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA. Translation: CAI86109.1.
RefSeqiWP_011327720.1. NC_007481.1.
YP_339552.1. NC_007481.1.

3D structure databases

ProteinModelPortaliQ3IJ93.
SMRiQ3IJ93. Positions 228-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326442.PSHAa1031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI86109; CAI86109; PSHAa1031.
GeneIDi3710242.
KEGGipha:PSHAa1031.
PATRICi32295589. VBIPseHal105694_0991.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-1038-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiSYL_PSEHT
AccessioniPrimary (citable) accession number: Q3IJ93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: May 27, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.