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Q3IJ93 (SYL_PSEHT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PSHAa1031
OrganismPseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]
Taxonomic identifier326442 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 862862Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009400

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3IJ93 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 323B8C27F25615BB

FASTA86297,721
        10         20         30         40         50         60 
MQEQYNPQKI ESKIQRYWEE NKVFKVTEDE SKEKYYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRFQRLKG KNVMQPMGWD AFGLPAENAA IKNKTAPAKW TYENIDYMRT QLKQLGFGYD 

       130        140        150        160        170        180 
WDREIATCHP EYYKWEQWFF TKLYEKGLVY KKMSTVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
AVVEQKEIPQ WFIKITDYAQ ELLDDLDKLE DWPEQVKTMQ RNWIGRSEGC DIEFKRTDNN 

       250        260        270        280        290        300 
ETFSVYTTRP DTFMGVTYVA VAAGHPIAKE AAKNSDSVAL FVEECKNNKV SEADMATMEK 

       310        320        330        340        350        360 
KGIATGFFAT HPLTGEQVPI WIANFVLMHY GSGAVMAVPA HDQRDFEFAT AYGLDIKQVI 

       370        380        390        400        410        420 
APVTGSDLEV NLNKEAFTDK GVLVNSGEFD GLDFDAAFNA IADKLEALGV GERKVNFRLR 

       430        440        450        460        470        480 
DWGVSRQRYW GSPIPMLSDE DGNELAATDD MLPVRLPEDV VMNGVTSPIK ADPNWAKTTM 

       490        500        510        520        530        540 
NGAPAFHETD TFDTFMESSW YYARYCSPRH DEGMLDPAAA NYWLPVNQYI GGIEHAILHL 

       550        560        570        580        590        600 
LYSRFFHKLL RDFGLVDSDE PFDRLLCQGM VLAETFYRKD EKGADIWISP SDVNTETDDK 

       610        620        630        640        650        660 
GRVTKAWHKV DGNPVFSSGM SKMSKSKNNG IDPQHVIAQY GADTVRLFMM FTAPPEQTLE 

       670        680        690        700        710        720 
WSDAGVEGAH RFLKRVWKYA VDVKTVGFQP LNKTALNNEQ KVLRRDLHKA IAKVSDDVER 

       730        740        750        760        770        780 
RQTFNTAIAA IMELSNKLLK APLNSKQDVA IANEALEAML IMLAPITPHL SHQLWQELGK 

       790        800        810        820        830        840 
EGDIVNASWP EVDQSALVED EKLIIIQVNG KLRAKLTVAA DATKEQVEAL AFEQASVTKY 

       850        860 
TDGVTVRKVI YVPGKLLNVV AN 

« Hide

References

[1]"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125."
Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. expand/collapse author list , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A.
Genome Res. 15:1325-1335(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TAC 125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR954246 Genomic DNA. Translation: CAI86109.1.
RefSeqYP_339552.1. NC_007481.1.

3D structure databases

ProteinModelPortalQ3IJ93.
SMRQ3IJ93. Positions 228-415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326442.PSHAa1031.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI86109; CAI86109; PSHAa1031.
GeneID3710242.
KEGGpha:PSHAa1031.
PATRIC32295589. VBIPseHal105694_0991.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPHAL326442:GJIU-1038-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSEHT
AccessionPrimary (citable) accession number: Q3IJ93
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries