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Q3IJ73

- PNP_PSEHT

UniProt

Q3IJ73 - PNP_PSEHT

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, PSHAa1001
Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881Magnesium By similarity
Metal bindingi494 – 4941Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. protein binding Source: IntAct
  5. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-1008-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:PSHAa1001
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000329777Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
rneQ3IHD22EBI-6412396,EBI-6412370

Protein-protein interaction databases

IntActiQ3IJ73. 1 interaction.
STRINGi326442.PSHAa1001.

Structurei

3D structure databases

ProteinModelPortaliQ3IJ73.
SMRiQ3IJ73. Positions 619-694.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KHAdd
BLAST
Domaini624 – 69269S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IJ73-1 [UniParc]FASTAAdd to Basket

« Hide

MQAIIKEFQL GQHTVTLETG AIARQADGAV LASIGDTSVL VTVVGKREAQ    50
PGQDFFPLTV NYQERMYAAG RIPGGFLKRE GRPNDGETLI ARLIDRPIRP 100
LFPSGFVNEV QVIATVVSVN PEIQPDMVAL IGTSAALAIS GIPFSGPIGA 150
TRVGYIDGEY VLNPTLKELE ESKLDLVVAG TDNAVLMVES EADVLAEDIM 200
LGAVVYGHEQ AQAIITAIKE FKAEAGKPTW DWTAPAKNVS LEEKVASIAA 250
DKVGEAYRIT DKVARKEALG VAKDEVVAVL TSELAEGESL DKQEIGKIFG 300
SLEKKIVRGR IAAGEKRIDG REPDMIRALD VMTGVLPRTH GSAIFTRGET 350
QALVTATLGT ERDSQLIDDL TGTHKNHFML NYNFPPFCVG ETGFVGSPKR 400
REIGHGNLAK RGIAAVMPTL TEFPYSIRVV SEITESNGSS SMASVCGTSL 450
ALMNAGVPIK ASVAGIAMGL VKEDDKFVVL SDILGDEDHL GDMDFKVAGT 500
AGGITALQMD IKIEGITQEI MQIALKQAKA ARLHILEVMD KAISAPSEEL 550
SQFAPRIYTM KIPQKKIAEV IGKGGATIRQ LTEETGTTIE IGDDGTIKIA 600
ATDGESAANA ISRIEQLTAE LEVGTIYEGK VVRIVDFGAF VNILPGKDGL 650
VHISQISTER VNNVTDHLSE GQEVKVKVLE VDRQGRVRLS IKEAMESAAP 700
AADAPTDA 708
Length:708
Mass (Da):75,599
Last modified:November 8, 2005 - v1
Checksum:iC3F49D29FA41D18A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI86079.1.
RefSeqiWP_011327690.1. NC_007481.1.
YP_339522.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI86079; CAI86079; PSHAa1001.
GeneIDi3709905.
KEGGipha:PSHAa1001.
PATRICi32295529. VBIPseHal105694_0961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI86079.1 .
RefSeqi WP_011327690.1. NC_007481.1.
YP_339522.1. NC_007481.1.

3D structure databases

ProteinModelPortali Q3IJ73.
SMRi Q3IJ73. Positions 619-694.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3IJ73. 1 interaction.
STRINGi 326442.PSHAa1001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI86079 ; CAI86079 ; PSHAa1001 .
GeneIDi 3709905.
KEGGi pha:PSHAa1001.
PATRICi 32295529. VBIPseHal105694_0961.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci PHAL326442:GJIU-1008-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiPNP_PSEHT
AccessioniPrimary (citable) accession number: Q3IJ73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 8, 2005
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi