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Q3IJ73

- PNP_PSEHT

UniProt

Q3IJ73 - PNP_PSEHT

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881MagnesiumUniRule annotation
Metal bindingi494 – 4941MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-1008-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:PSHAa1001
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Polyribonucleotide nucleotidyltransferasePRO_0000329777Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
rneQ3IHD22EBI-6412396,EBI-6412370

Protein-protein interaction databases

IntActiQ3IJ73. 1 interaction.
STRINGi326442.PSHAa1001.

Structurei

3D structure databases

ProteinModelPortaliQ3IJ73.
SMRiQ3IJ73. Positions 619-694.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KHUniRule annotationAdd
BLAST
Domaini624 – 69269S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IJ73 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQAIIKEFQL GQHTVTLETG AIARQADGAV LASIGDTSVL VTVVGKREAQ
60 70 80 90 100
PGQDFFPLTV NYQERMYAAG RIPGGFLKRE GRPNDGETLI ARLIDRPIRP
110 120 130 140 150
LFPSGFVNEV QVIATVVSVN PEIQPDMVAL IGTSAALAIS GIPFSGPIGA
160 170 180 190 200
TRVGYIDGEY VLNPTLKELE ESKLDLVVAG TDNAVLMVES EADVLAEDIM
210 220 230 240 250
LGAVVYGHEQ AQAIITAIKE FKAEAGKPTW DWTAPAKNVS LEEKVASIAA
260 270 280 290 300
DKVGEAYRIT DKVARKEALG VAKDEVVAVL TSELAEGESL DKQEIGKIFG
310 320 330 340 350
SLEKKIVRGR IAAGEKRIDG REPDMIRALD VMTGVLPRTH GSAIFTRGET
360 370 380 390 400
QALVTATLGT ERDSQLIDDL TGTHKNHFML NYNFPPFCVG ETGFVGSPKR
410 420 430 440 450
REIGHGNLAK RGIAAVMPTL TEFPYSIRVV SEITESNGSS SMASVCGTSL
460 470 480 490 500
ALMNAGVPIK ASVAGIAMGL VKEDDKFVVL SDILGDEDHL GDMDFKVAGT
510 520 530 540 550
AGGITALQMD IKIEGITQEI MQIALKQAKA ARLHILEVMD KAISAPSEEL
560 570 580 590 600
SQFAPRIYTM KIPQKKIAEV IGKGGATIRQ LTEETGTTIE IGDDGTIKIA
610 620 630 640 650
ATDGESAANA ISRIEQLTAE LEVGTIYEGK VVRIVDFGAF VNILPGKDGL
660 670 680 690 700
VHISQISTER VNNVTDHLSE GQEVKVKVLE VDRQGRVRLS IKEAMESAAP

AADAPTDA
Length:708
Mass (Da):75,599
Last modified:November 8, 2005 - v1
Checksum:iC3F49D29FA41D18A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI86079.1.
RefSeqiYP_339522.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI86079; CAI86079; PSHAa1001.
GeneIDi3709905.
KEGGipha:PSHAa1001.
PATRICi32295529. VBIPseHal105694_0961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI86079.1 .
RefSeqi YP_339522.1. NC_007481.1.

3D structure databases

ProteinModelPortali Q3IJ73.
SMRi Q3IJ73. Positions 619-694.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3IJ73. 1 interaction.
STRINGi 326442.PSHAa1001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI86079 ; CAI86079 ; PSHAa1001 .
GeneIDi 3709905.
KEGGi pha:PSHAa1001.
PATRICi 32295529. VBIPseHal105694_0961.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci PHAL326442:GJIU-1008-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiPNP_PSEHT
AccessioniPrimary (citable) accession number: Q3IJ73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3