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Q3IJ73

- PNP_PSEHT

UniProt

Q3IJ73 - PNP_PSEHT

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi488 – 4881MagnesiumUniRule annotation
    Metal bindingi494 – 4941MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. protein binding Source: IntAct
    5. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciPHAL326442:GJIU-1008-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:PSHAa1001
    OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
    Taxonomic identifieri326442 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 708708Polyribonucleotide nucleotidyltransferasePRO_0000329777Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rneQ3IHD22EBI-6412396,EBI-6412370

    Protein-protein interaction databases

    IntActiQ3IJ73. 1 interaction.
    STRINGi326442.PSHAa1001.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3IJ73.
    SMRiQ3IJ73. Positions 619-694.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini555 – 61460KHUniRule annotationAdd
    BLAST
    Domaini624 – 69269S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218327.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    Q3IJ73-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAIIKEFQL GQHTVTLETG AIARQADGAV LASIGDTSVL VTVVGKREAQ    50
    PGQDFFPLTV NYQERMYAAG RIPGGFLKRE GRPNDGETLI ARLIDRPIRP 100
    LFPSGFVNEV QVIATVVSVN PEIQPDMVAL IGTSAALAIS GIPFSGPIGA 150
    TRVGYIDGEY VLNPTLKELE ESKLDLVVAG TDNAVLMVES EADVLAEDIM 200
    LGAVVYGHEQ AQAIITAIKE FKAEAGKPTW DWTAPAKNVS LEEKVASIAA 250
    DKVGEAYRIT DKVARKEALG VAKDEVVAVL TSELAEGESL DKQEIGKIFG 300
    SLEKKIVRGR IAAGEKRIDG REPDMIRALD VMTGVLPRTH GSAIFTRGET 350
    QALVTATLGT ERDSQLIDDL TGTHKNHFML NYNFPPFCVG ETGFVGSPKR 400
    REIGHGNLAK RGIAAVMPTL TEFPYSIRVV SEITESNGSS SMASVCGTSL 450
    ALMNAGVPIK ASVAGIAMGL VKEDDKFVVL SDILGDEDHL GDMDFKVAGT 500
    AGGITALQMD IKIEGITQEI MQIALKQAKA ARLHILEVMD KAISAPSEEL 550
    SQFAPRIYTM KIPQKKIAEV IGKGGATIRQ LTEETGTTIE IGDDGTIKIA 600
    ATDGESAANA ISRIEQLTAE LEVGTIYEGK VVRIVDFGAF VNILPGKDGL 650
    VHISQISTER VNNVTDHLSE GQEVKVKVLE VDRQGRVRLS IKEAMESAAP 700
    AADAPTDA 708
    Length:708
    Mass (Da):75,599
    Last modified:November 8, 2005 - v1
    Checksum:iC3F49D29FA41D18A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954246 Genomic DNA. Translation: CAI86079.1.
    RefSeqiWP_011327690.1. NC_007481.1.
    YP_339522.1. NC_007481.1.

    Genome annotation databases

    EnsemblBacteriaiCAI86079; CAI86079; PSHAa1001.
    GeneIDi3709905.
    KEGGipha:PSHAa1001.
    PATRICi32295529. VBIPseHal105694_0961.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954246 Genomic DNA. Translation: CAI86079.1 .
    RefSeqi WP_011327690.1. NC_007481.1.
    YP_339522.1. NC_007481.1.

    3D structure databases

    ProteinModelPortali Q3IJ73.
    SMRi Q3IJ73. Positions 619-694.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q3IJ73. 1 interaction.
    STRINGi 326442.PSHAa1001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI86079 ; CAI86079 ; PSHAa1001 .
    GeneIDi 3709905.
    KEGGi pha:PSHAa1001.
    PATRICi 32295529. VBIPseHal105694_0961.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218327.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci PHAL326442:GJIU-1008-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TAC 125.

    Entry informationi

    Entry nameiPNP_PSEHT
    AccessioniPrimary (citable) accession number: Q3IJ73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi