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Q3IIZ7

- GLND_PSEHT

UniProt

Q3IIZ7 - GLND_PSEHT

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-2090-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:PSHAa2038
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 872872Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231685Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi326442.PSHAa2038.

Structurei

3D structure databases

ProteinModelPortaliQ3IIZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini452 – 582131HDUniRule annotationAdd
BLAST
Domaini692 – 77382ACT 1UniRule annotationAdd
BLAST
Domaini799 – 87274ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseAdd
BLAST
Regioni333 – 691359Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IIZ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALPNKVKKL LSDAEQLSDY RDCSSYFYKW LFNEFSKQPV GNLINARAEF
60 70 80 90 100
IDRLLIKLFH VYDLAHEPDL ALIAVGGYGR GELHPYSDID FLLLVTQQPN
110 120 130 140 150
AEISEKIGQF VTMLWDLNLE IGHSVRTIAQ AIEQKREDVT FATSLLESRL
160 170 180 190 200
IFGNHIEFEK LKNHIIDTPV WRSNEFFLAK VQEQHLRHRK CHGTAYNLEP
210 220 230 240 250
NIKENPGGLR DLQTIIWVAK KHFRAETLQE LISHGYLTHE EFQELSECLE
260 270 280 290 300
NLWNIRFALH LAAGRSENRL LFDHQPQAAE ILGFGSDGKS SVERMMKRLF
310 320 330 340 350
RIMSRVRELN LMLLAYFEQS ISPKHHQPII QELDRNFERI GNQIKVKSPS
360 370 380 390 400
VFFRRDQLFM LFEHIADNPE ITHIYPSTIR TIRQVRRRLL GDLQDYAACR
410 420 430 440 450
EAFLRLIKHP NGMGRAFTLM HKHGMLAAYL PQWRNIFGQM QFDLFHAYTV
460 470 480 490 500
DEHTHRLINN IYQYFDKTGV SEFPICSEIV TRMDKPELLY LAGIFHDIAK
510 520 530 540 550
GRGGDHSELG AVDALAFAKL HAFSVADGKL IAWLVSNHLL MSVTAQRKDI
560 570 580 590 600
NDPGVIKDFA TRVKTERQLD YLYCLTVADI RATNDNLWND WKNTLLRELY
610 620 630 640 650
LHTQHALRLG LENPMDQRDQ IRDKKHQAKQ RLLNLGYMED QIDLIWSRFK
660 670 680 690 700
ANYFTAFSEQ QISWHSQHLV NSEDLSQPSV IVSNKAMHGG TQVFVYSPYS
710 720 730 740 750
GPLFARLVSV IGSKKAQIQH AQVLTTKDGY VLFNFVILEV NGEPIASGRA
760 770 780 790 800
QSIKRALEQA LFEPRKKIRF KKNRSQRFKD FNIKPKIVLR PHPRKDRSLI
810 820 830 840 850
EIQAIDIPGL LTKIAEVFQA HLLHIHAARI TTVGQRAEDF FVVSNNEYQA
860 870
LTDEEQAKIH QALRKKLNAE TE
Length:872
Mass (Da):101,049
Last modified:November 8, 2005 - v1
Checksum:i473147E4F7F35DA0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI87094.1.
RefSeqiWP_011328696.1. NC_007481.1.
YP_340537.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI87094; CAI87094; PSHAa2038.
GeneIDi3708244.
KEGGipha:PSHAa2038.
PATRICi32297675. VBIPseHal105694_1966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI87094.1 .
RefSeqi WP_011328696.1. NC_007481.1.
YP_340537.1. NC_007481.1.

3D structure databases

ProteinModelPortali Q3IIZ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326442.PSHAa2038.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI87094 ; CAI87094 ; PSHAa2038 .
GeneIDi 3708244.
KEGGi pha:PSHAa2038.
PATRICi 32297675. VBIPseHal105694_1966.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PHAL326442:GJIU-2090-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiGLND_PSEHT
AccessioniPrimary (citable) accession number: Q3IIZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3