Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathway: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-2069-MONOMER.
UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:PSHAa2017
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Lipid-A-disaccharide synthasePRO_0000255206Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi326442.PSHAa2017.

Structurei

3D structure databases

ProteinModelPortaliQ3IIW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018003.
KOiK00748.
OMAiQPWVGLP.
OrthoDBiEOG6FBWZR.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3IIW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKDQKQLRI GIVAGELSGD ILGEGLIKAL KKHFPDAIFE GIAGPKMQAQ
60 70 80 90 100
GCNTLYDMDE LSVMGLVEVL GRLPRLLKIR KQLVQHFIDN PPDVFIGIDA
110 120 130 140 150
PDFNLRVEKP LKDAGIKTVQ YVSPSVWAWR EKRIHTISAA TNLVLALLPF
160 170 180 190 200
EKEFYDKHQV PCTFVGHTLA DDIALEHDDS KARKELGLSP DDKVLALLPG
210 220 230 240 250
SRGSEVGLLS ETYIKTAVQL QAQNPALKIV VPLVNAKRKA QFTEILNATA
260 270 280 290 300
PTLKISLLDG QSKQAMQAAD AILLASGTAT LEGMLYKKPM VVGYKIKPLS
310 320 330 340 350
YWIFKTLFTF NIKYFSLPNL LADEELVPEF LQSECNVANL TQALTPMLNT
360 370 380
DNQALKARFL AIHKKIRLNA SEQAANAVAE LINAN
Length:385
Mass (Da):42,491
Last modified:November 8, 2005 - v1
Checksum:i5EB17FDA1F34F8B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA. Translation: CAI87073.1.
RefSeqiWP_011328675.1. NC_007481.1.
YP_340516.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI87073; CAI87073; PSHAa2017.
GeneIDi3710256.
KEGGipha:PSHAa2017.
PATRICi32297631. VBIPseHal105694_1944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA. Translation: CAI87073.1.
RefSeqiWP_011328675.1. NC_007481.1.
YP_340516.1. NC_007481.1.

3D structure databases

ProteinModelPortaliQ3IIW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326442.PSHAa2017.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI87073; CAI87073; PSHAa2017.
GeneIDi3710256.
KEGGipha:PSHAa2017.
PATRICi32297631. VBIPseHal105694_1944.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018003.
KOiK00748.
OMAiQPWVGLP.
OrthoDBiEOG6FBWZR.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.
BioCyciPHAL326442:GJIU-2069-MONOMER.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiLPXB_PSEHT
AccessioniPrimary (citable) accession number: Q3IIW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 8, 2005
Last modified: April 29, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.