Reviewed, UniProtKB/Swiss-Prot Q3II62 (SYY1_PSEHT)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase 1
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 1
      Short name=TyrRS 1

Gene names

Name:	tyrS1
Ordered Locus Names:	PSHAa2413

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Tyrosyl-tRNA synthetase 1 HAMAP MF_02006		PRO_0000234752
Regions
	Domain	355 – 419	65	S4 RNA-binding
	Motif	40 – 49	10	"HIGH" region HAMAP MF_02006
	Motif	232 – 236	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	35	1	Tyrosine By similarity
	Binding site	172	1	Tyrosine By similarity
	Binding site	176	1	Tyrosine By similarity
	Binding site	235	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3II62-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 66DFB6C036EC6824	FASTA	420	46,895
	10 20 30 40 50 60 MTTQLLEDIT HRGLVSQVSD LAQLEQLLAT SQVVYCGFDP TAGSLHIGHL VPLLMLKRFN 70 80 90 100 110 120 DAGHKAVALI GGATGLIGDP SFKATERSLN SKETVQGWVA DLSSQVESVM NPHLSEPIQL 130 140 150 160 170 180 KNNADWFSGI EVLDFFRDVG KHFSINNMIN RESVKQRLQR PDQGLSFTEF SYTLLQSYDF 190 200 210 220 230 240 AKLNSELGCS VQIGGNDQWG NIVSGIDLTR RLNKQTVYGL TLPLITKSDG TKFGKTEGGA 250 260 270 280 290 300 IWLDPKKTSP YRFYQFWLNC DDADVYNFLR FYTFLSVKEI EAIEANDITS QQKPQAQGIL 310 320 330 340 350 360 AEQLTRFVHG EQGLASAQRI TQLLFNGQVQ TLTLAELEQL EQDGLAVHAI SDAKINIAEL 370 380 390 400 410 420 LVKSELASSK RTARELINAN AIKINGEAIT DEHAQLDFPL FDRFWVMQRG KKQFRLIKLA

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR954246 Genomic DNA. Translation: CAI87462.1.
RefSeq	YP_340904.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3II62.
Genome annotation databases
GeneID	3710330.
GenomeReviews	Gene locus PSHAa2413 in contig CR954246_GR.
KEGG	pha:PSHAa2413.
NMPDR	fig|326442.4.peg.2503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3II62.
OMA	TFYIGFD.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA2413-MON.
Family and domain databases
HAMAP	MF_02006. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY1_PSEHT

Accession

Primary (citable) accession number: Q3II62

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)