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Q3IHZ2 (PUR4_PSEHT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:PSHAa2330
OrganismPseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]
Taxonomic identifier326442 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length1296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12961296Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000264586

Regions

Domain1043 – 1296254Glutamine amidotransferase type-1
Nucleotide binding304 – 31512ATP Potential

Sites

Active site11361Nucleophile By similarity
Active site12611 By similarity
Active site12631 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3IHZ2 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 2F7EBAF8E8FFDBFC

FASTA1,296140,882
        10         20         30         40         50         60 
MLILRGAPAL SEFRVNKILA RCQQSQLPVT NVYAEYAHFA DLTSPLSSAE QTKLEKLLTY 

        70         80         90        100        110        120 
GPTIAEHTPA GKLVLVTPRP GTISPWASKA TDIAHNCGLK QVHRVERGIA YYVEGELNAE 

       130        140        150        160        170        180 
QLLQVTALLH DRMTEATHSQ FEDAAQLFRS DAPRQMSSVD ILSGGREALA IANVEQGFAL 

       190        200        210        220        230        240 
ADDEIDYLVE NFIKLGRNPN DIELFMFAQA NSEHCRHKIF NADWTIDGEE QPKSLFKMIK 

       250        260        270        280        290        300 
NTFEKNPENV LSAYKDNAAV MKGSKAGRFF PNTQGEYAYH QEDIEILMKV ETHNHPTAIA 

       310        320        330        340        350        360 
PFSGAATGSG GEIRDEGATG RGSKPKAGLV GFTVSNLRIP GYEQPWESDF GKPGRIVTAL 

       370        380        390        400        410        420 
DIMTEGPLGG AAFNNEFGRP NLLGYFRTYE EQVTSHNGLE VRGYHKPIML AGGLGNIRTD 

       430        440        450        460        470        480 
HVQKGEIPVG AKLIALGGPA MNIGLGGGAA SSMASGQSNE DLDFASVQRE NPEMERRCQE 

       490        500        510        520        530        540 
VIDKCWQLGD ENPIAFIHDV GAGGLSNAFP ELVNDGGRGG KFQLRDIPND EPGMAPHEIW 

       550        560        570        580        590        600 
CNESQERYVL AVGVEDFDRF EAICKRERAQ YAVIGEATAE PHLTVADSHF DNNPVDLPLD 

       610        620        630        640        650        660 
VLLGKAPKMH RDVTSKQVVG KALDVTNINV ADAAQRLLRL PTIAEKTFLI TIGDRSVTGL 

       670        680        690        700        710        720 
VARDQMVGPW QVPVANCAVT AATYDTYHGE AMSLGERTPA ALLNYAASAR LAVAESLTNI 

       730        740        750        760        770        780 
ACANIGSLEN IKLSANWMAA AGHPGEDAGL YEAVKAIGEE LCPALGLTIP VGKDSMSMKT 

       790        800        810        820        830        840 
TWKDEGDSQE KSVTSPLSLI ITAFGRVDDV RKTVTPQLRT DKGETSLILV DLGAGKNRMG 

       850        860        870        880        890        900 
ASSLAQVYKQ LGDITPDVDS PELLKGFYNA MQVLVADSKL LAYHDRSDGG LFTTVAEMAF 

       910        920        930        940        950        960 
AGHTGVTVDI NGLTGNDIEA LYNEELGAVI QVANSDLDAV NAVLKDHGLA TISHIIGTLN 

       970        980        990       1000       1010       1020 
SDDAIVFNRG KNTVLSNTRT ELRTMWAETT YQMQARRDNP ECAKQEFDAK FDVKDPGLNV 

      1030       1040       1050       1060       1070       1080 
KLNFDLNEDI AAPYIATGAK PPMAILREQG VNSHLEMAAA FNRAGFAAID VHMSDILEGR 

      1090       1100       1110       1120       1130       1140 
LSLEQFKGLV ACGGFSYGDV LGAGEGWAKS ILFNDMAREQ FQSFFHREDT FSLGVCNGCQ 

      1150       1160       1170       1180       1190       1200 
MLSTLKELIP GTEHWPRFVT NKSERFEARF SLVEIQENPS VFFNGMAGSR MPIAVSHGEG 

      1210       1220       1230       1240       1250       1260 
HAEFANDNAV KAALDSGTVA VKFVDNYGNP TTQYPANPNG SPEGITGITS TDGRATVMMP 

      1270       1280       1290 
HPERVFRAVA NSWHPDEWRE DSPWMRMFRN ARKNVG

« Hide

References

[1]"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125."
Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. expand/collapse author list , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A.
Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TAC 125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR954246 Genomic DNA. Translation: CAI87386.1.
RefSeqYP_340828.1. NC_007481.1.

3D structure databases

HSSPHSSP built from PDB template 1VK3 based on UniProtKB Q9X0X3.
ProteinModelPortalQ3IHZ2.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ3IHZ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3709939.
GenomeReviewsGene locus PSHAa2330 in contig CR954246_GR.
KEGGpha:PSHAa2330.
NMPDRfig|326442.4.peg.2380.
PATRIC32298257. VBIPseHal105694_2252.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
OMAVKAVGME.
PhylomeDBQ3IHZ2.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycPHAL326442:PSHAA2330-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_PSEHT
AccessionPrimary (citable) accession number: Q3IHZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 8, 2005
Last modified: January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents