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Protein

Membrane-bound lytic murein transglycosylase F

Gene

mltF

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.UniRule annotation

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei304UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciPHAL326442:G1GI4-2308-MONOMER

Protein family/group databases

CAZyiGH23 Glycoside Hydrolase Family 23

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase FUniRule annotation (EC:4.2.2.n1UniRule annotation)
Alternative name(s):
Murein lyase FUniRule annotation
Gene namesi
Name:mltFUniRule annotation
Ordered Locus Names:PSHAa2331
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
Proteomesi
  • UP000006843 Componenti: Chromosome I

Subcellular locationi

  • Cell outer membrane ; Peripheral membrane protein
  • Note: Attached to the inner leaflet of the outer membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotationAdd BLAST21
ChainiPRO_000035396022 – 470Membrane-bound lytic murein transglycosylase FAdd BLAST449

Proteomic databases

PRIDEiQ3IHZ1

Interactioni

Protein-protein interaction databases

STRINGi326442.PSHAa2331

Structurei

3D structure databases

ProteinModelPortaliQ3IHZ1
SMRiQ3IHZ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 259Non-LT domainUniRule annotationAdd BLAST238
Regioni260 – 470LT domainUniRule annotationAdd BLAST211

Domaini

The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.UniRule annotation
In the C-terminal section; belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CHQ Bacteria
COG4623 LUCA
HOGENOMiHOG000218316
KOiK18691
OMAiYYDILTW
OrthoDBiPOG091H03Q9

Family and domain databases

HAMAPiMF_02016 MltF, 1 hit
InterProiView protein in InterPro
IPR023346 Lysozyme-like_dom_sf
IPR023703 MltF
IPR001638 Solute-binding_3/MltF_N
IPR000189 Transglyc_AS
IPR008258 Transglycosylase_SLT_dom_1
PfamiView protein in Pfam
PF00497 SBP_bac_3, 1 hit
PF01464 SLT, 1 hit
SMARTiView protein in SMART
SM00062 PBPb, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit
PS00922 TRANSGLYCOSYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3IHZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKEKLIIII TLVMLLCACD IQEQSTQLAQ IKAQNKIRVG TLASASNYYQ
60 70 80 90 100
AVQGEQGFEY ELSQAFADYL GVELEIVPFF NLSDMFARLQ SGDLDLIASG
110 120 130 140 150
LTYNKTRAQQ YRFGPTYRTI SQKLVYKQGI ERPRDYDDLT GNLMVIAKSS
160 170 180 190 200
HSLTLQKVKK SNPELNWSET EEFDEEELLQ AVIDGEIDYT LADTHTLSLF
210 220 230 240 250
RRYHPNLSIG FSITHNDPIA WMLRKSNDDS LYALLVPFFG EAKQNNQLYV
260 270 280 290 300
LEEKYFGHVR QFNYVNTLAY IEAIKETLPK YQPWFEQYAG TLDWRLLAAL
310 320 330 340 350
SYQESMWNPR AKSPTGVRGI MMLTRNTAKQ VGVTNRLEPE QNIRGGAKYL
360 370 380 390 400
SKLIKRIPDR IPQPDRTWLA LAAYNVGWGH VNDARIITKQ QGASPDKWAD
410 420 430 440 450
VKKRLPLLIK KRYYRKTRYG YSRGDVAVSY VDNIRRYYDA LVWLDENNAI
460 470
EEKPEAPVVA PKIGDEVEAK
Length:470
Mass (Da):54,060
Last modified:November 8, 2005 - v1
Checksum:i562C3071C0256085
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA Translation: CAI87387.1
RefSeqiWP_011328988.1, NC_007481.1

Genome annotation databases

EnsemblBacteriaiCAI87387; CAI87387; PSHAa2331
KEGGipha:PSHAa2331
PATRICifig|326442.8.peg.2253

Similar proteinsi

Entry informationi

Entry nameiMLTF_PSEHT
AccessioniPrimary (citable) accession number: Q3IHZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 8, 2005
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health