Reviewed, UniProtKB/Swiss-Prot Q3IHW0 (TDH_PSEHT)

Names and origin

Protein names

Recommended name:
    L-threonine 3-dehydrogenase
    EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	PSHAa2315

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_1000051646
Sites
	Metal binding	38	1	Zinc 1; catalytic By similarity
	Metal binding	63	1	Zinc 1; catalytic By similarity
	Metal binding	93	1	Zinc 2 By similarity
	Metal binding	96	1	Zinc 2 By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	148	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IHW0-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 78E3E098A8EC6F60	FASTA	341	37,262
	10 20 30 40 50 60 MKALSKLKAE PGIWMTDAPK PEVGHNDLLI KIRKTAICGT DVHIYKWDEW AQKTIPTPMV 70 80 90 100 110 120 VGHEYVGEVV DMGQEVRGFK VGDRVSGEGH ITCGHCRNCR AGRVHLCRNT TGVGVNREGA 130 140 150 160 170 180 FAEYLVIPAF NAFKIPDNIS DELASIFDPF GNAVHTALSF DLVGEDVLIT GAGPIGIMAA 190 200 210 220 230 240 AVAKHVGARH VVISDVNEYR LELARKMGAT RAVNVANEKL EDVIKELGMT EGFDIGLEMS 250 260 270 280 290 300 GVPSAFNSML NNMNHGGKVA MLGIPPSDMA VDWNQVIFKG LVIKGIYGRE MFETWYKMAS 310 320 330 340 LIQSGLDLNP IITHQYSIDD FQAGFDMMIS GQSGKVILNW D

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR954246 Genomic DNA. Translation: CAI87371.1.
RefSeq	YP_340813.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IHW0.
Genome annotation databases
GeneID	3709585.
GenomeReviews	Gene locus PSHAa2315 in contig CR954246_GR.
KEGG	pha:PSHAa2315.
NMPDR	fig|326442.4.peg.2328.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3IHW0.
OMA	IWMVDAP.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA2315-MON.
Family and domain databases
HAMAP	MF_00627. [Tree]
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR004627. L-Threonine_3-DHase. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProDom	PD040557. GroES_related. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00692. tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_PSEHT

Accession

Primary (citable) accession number: Q3IHW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)