Q3IHQ6 (RLMG_PSEHT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	PSHAa2245

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366471

Sequences

	Sequence		Length	Mass (Da)
	Q3IHQ6 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: D6891A25E1B5E47F	FASTA	382	42,574
	10 20 30 40 50 60 MTMQAQLGDK TFTLERFPLD QKNRSLQAWD SADEYLIDYV SEHHPDCRSL LILNDSFGAL 70 80 90 100 110 120 SCYFSHKQLT VCSVNDSYIS HQAAAYNLAE NKIPAITFSQ LDSLSALPEQ VDLVLIKVPR 130 140 150 160 170 180 TLGFLQYQLS ELSEVLAPGT PVIAAAKTKD VHNSTIKAFE QFIGETKTSL AVKKSRLIIS 190 200 210 220 230 240 HAQGGYKAAD FPVNWPLEGT DFTISNHANV FSRDSLDIGA RFFFNYLPET NKAKSIIDLG 250 260 270 280 290 300 CGNGVVGLMA LSRCPNANIT FVDESYMAVE SARLNVELNM EAKYEQCSFV ENDCLSGFER 310 320 330 340 350 360 DSVDMVLCNP PFHQAQAVTD HIAWQMFKEA KDTLKEGGEL RIIGNRHLDY HDKLNRMFGN 370 380 CKLLGSNKKF VVLSATKNTG ML

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TAC 125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954246 Genomic DNA. Translation: CAI87301.1.
RefSeq	YP_340743.1. NC_007481.1.
3D structure databases
ProteinModelPortal	Q3IHQ6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IHQ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3710691.
GenomeReviews	Gene locus PSHAa2245 in contig CR954246_GR.
KEGG	pha:PSHAa2245.
NMPDR	fig|326442.4.peg.2215.
PATRIC	32298085. VBIPseHal105694_2166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
PhylomeDB	Q3IHQ6.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA2245-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEHT

Accession

Primary (citable) accession number: Q3IHQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program