Reviewed, UniProtKB/Swiss-Prot Q3IHK8 (PROB2_PSEHT)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase 2
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase 2
      Short name=GK 2

Gene names

Name:	proB2
Ordered Locus Names:	PSHAa0633

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Glutamate 5-kinase 2 HAMAP MF_00456		PRO_0000230056
Regions
	Domain	275 – 353	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q3IHK8-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: CE911995481AF222	FASTA	367	39,084
	10 20 30 40 50 60 MQRDQVIVVK LGTSVLTGGT DKLDKAHMVE LVRQCCELKK QGHHVILVSS GAVAAGREQL 70 80 90 100 110 120 LKPCGRSVID KQMLAAVGQG QLIHIWQSLF ALYGVNVGQM LLTRADVNDR ERYLNARDTL 130 140 150 160 170 180 NALLNYDVVP IINENDAVAT SEIKVGDNDN LSALVAILAN ANKLLLLTDQ EGLFTSDPRT 190 200 210 220 230 240 NADATLIGEV SDINDELRQL AGGSGTNLGT GGMATKLQAA DIARRAGVEV IIAKGAGKNV 250 260 270 280 290 300 ILKCMSEQLP GTRFLKLTAP KEGRKKWLLA GPKSSGQIVI DAGAITALQT KGASLLAKGV 310 320 330 340 350 360 TNALGAFERG DLISVINSDK QLIARGLTRF SSSEVNKIKG AHSKQIGELL DYDGGAEVLH RDDLILL

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR954246 Genomic DNA. Translation: CAI85718.1.
RefSeq	YP_339161.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IHK8.
Genome annotation databases
GeneID	3709848.
GenomeReviews	Gene locus PSHAa0633 in contig CR954246_GR.
KEGG	pha:PSHAa0633.
NMPDR	fig|326442.4.peg.604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3IHK8.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA0633-MON.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB2_PSEHT

Accession

Primary (citable) accession number: Q3IHK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)