Reviewed, UniProtKB/Swiss-Prot Q3IHD9 (ACEK2_PSEHT)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase 2
      Short name=IDH kinase/phosphatase 2
      Short name=IDHK/P 2
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK2
Ordered Locus Names:	PSHAa1821

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Isocitrate dehydrogenase kinase/phosphatase 2 HAMAP MF_00747		PRO_0000288294
Regions
	Nucleotide binding	313 – 319	7	ATP By similarity
Sites
	Active site	369	1	By similarity
	Binding site	334	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IHD9-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 73397FF3DE21EDBF	FASTA	571	66,954
	10 20 30 40 50 60 MQPRHIAELI LTGFKKHYLL FQKTTAKAPL AFAKRDWQAI NDISRLRISH YDDRVNETTA 70 80 90 100 110 120 TLRQQQTEQL DEQLWLEVKK LYQHFLCFHP QAELAETFYN SVFCRLYHRR YFHNDFIFVE 130 140 150 160 170 180 ATLKDAPAVP VEAEYRSYFP VVDGLKPTIK QIINHFDFKA PFVNLERDIR LLVKAFYKQA 190 200 210 220 230 240 PDTHHKAWQM RFDILHTPFY RNKAAYIVGR VVSQSGVQPF IIAVLHHEDK GLYLDALLTK 250 260 270 280 290 300 SSQMRVIFGF ARAYFMVETH APCALVRFLN QLMPNKTIAE LYNAIGFHKQ GKTEFYREFL 310 320 330 340 350 360 NHLTHSNDEF TIAPGTPGMV MMVFTLPSFG YVFKVIKDKF GESKPFGRDT VLKRYQLVKK 370 380 390 400 410 420 HDRVGRMADT IEYSNVVFPL ARFDSNLLQQ LHQTIGSSMV IEGDWLIIKH LYIERRMTPL 430 440 450 460 470 480 NLFLENADDA SAADAIEEYG QALKEMIAVN IFPGDMLLKN FGVSKHKRII FYDYDEVQYL 490 500 510 520 530 540 TDMNFRALPK AKTYDDYLMD EQSYSVAPQD VFPEQLCTFV MPNPIYKQFL MSTHPELIDV 550 560 570 NFWKQAQQNI KNGQVSHIYP YPTAQRFIHH W

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR954246 Genomic DNA. Translation: CAI86893.1.
RefSeq	YP_340336.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IHD9.
Genome annotation databases
GeneID	3709030.
GenomeReviews	Gene locus PSHAa1821 in contig CR954246_GR.
KEGG	pha:PSHAa1821.
NMPDR	fig|326442.4.peg.1859.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3IHD9.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA1821-MON.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK2_PSEHT

Accession

Primary (citable) accession number: Q3IHD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)