Reviewed, UniProtKB/Swiss-Prot Q3IFM1 (CYSI_PSEHT)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	PSHAa0155

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000292962
Sites
	Metal binding	429	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	435	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	474	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	478	1	Iron (siroheme axial ligand) By similarity
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IFM1-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: F622A443A6723502	FASTA	565	62,933
	10 20 30 40 50 60 MSEQDKNVKL SDNERMKRES NFLRGTIATD LKDEITGGFT ADNFQLIRFH GMYQQDDRDI 70 80 90 100 110 120 RGERAKQKLE PLHNVMLRAR MPGGIIKPEQ WLAIDKFAEE KTSYGSIRLT TRQTFQFHGV 130 140 150 160 170 180 LKPNIKGMHQ LLDSVGIDSI ATAGDVNRNV LCTTNPVESE LHQEAYEWAA KISEHLLPKT 190 200 210 220 230 240 KAYAEIWLNG EKAETTEEPI LGSNYLPRKF KTTVTIPPNN EVDVHANDLN FVAIAENGKL 250 260 270 280 290 300 IGFNVLVGGG LAMTHGDTAT YPRKADDFGF IPLEHTLKIA EHVVSVQRDW GNRSNRKNAK 310 320 330 340 350 360 TKYTLDRVGV DVFKAEVEKR AGVEFASSRP YKFTHRGDRI GWVEGIDGKH HLTLFIQSGR 370 380 390 400 410 420 ILDYPDKPLK TGCRKIAEVH QGDMRMTANQ NLIIAGVAAN QKAVIEEIAR NHGLIDDKDT 430 440 450 460 470 480 EQRKNSMACV ALPTCPLAMA EAERYLPSLV EKIEALLAKH GVPNDSIIMR VVGCPNGCGR 490 500 510 520 530 540 AMLAEAGLVG KGPGKYNVYL GGNLEGTRIP KLYLENVGED VYLAAFDELI GQWVNERNDG 550 560 ECFGDFVIRK GIVAEVKVSV TDFHA

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR954246 Genomic DNA. Translation: CAI85259.1.
RefSeq	YP_338702.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3709416.
GenomeReviews	Gene locus PSHAa0155 in contig CR954246_GR.
KEGG	pha:PSHAa0155.
NMPDR	fig|326442.4.peg.240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3IFM1.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA0155-MON.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_PSEHT

Accession

Primary (citable) accession number: Q3IFM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	November 8, 2005
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)