Reviewed, UniProtKB/Swiss-Prot Q3IF27 (PLSB_PSEHT)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PSHAa0142

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 812	812	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049446
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q3IF27-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 1751179DED26D381	FASTA	812	92,076
	10 20 30 40 50 60 MRIVNYCLNV LSAGVSRLLV RSKVLPEKPT EQYELNSKNP TFYIVRLNSR FDLAALARVC 70 80 90 100 110 120 KRHGLPDPRE QQVLGTQRLD RFIGIENPPP LIGDIAKPTN ALAQGKQIID HLLSSGQKDV 130 140 150 160 170 180 QVIPVTILWG RAPGKEKPGL STLITHSLTP SWFRKLFVVL FSGRDNFIRF SQPLDLSLLV 190 200 210 220 230 240 DEKADVNELP QKLLRVARVH FRRQKLAATG PKMPSREQLF NSLLASPTIK KAIQDEAKAK 250 260 270 280 290 300 NISQAQARQN AVKLLNEIAA NYSEAMIRVA ERFLTWLWNK LYNGIDIKYT EQIHELTNKG 310 320 330 340 350 360 HEIIYMPCHR SHMDYLLLTY AIYHQGLVPP HIAAGINLNF FPAGGIFRRS GAFFIRRSFA 370 380 390 400 410 420 GNKLYSAVFK EYLSQLFIKG YSVKFYTEGG RSRTGRLLPP KTGMLAMTMQ AMLRGIDRPI 430 440 450 460 470 480 SIVPVYIGYE HVMEINTYLK ELAGNDKKGE SIFGIFKAIK NLKNYGRGYL NFGDPISINQ 490 500 510 520 530 540 YLNDNQPNWR DDIHPTDVQK PQWLGPQVAN LADQVMVKIN NAAALNAVNL LAMILLVNDK 550 560 570 580 590 600 HALSKPKLLA QLDFYLRLQR DASYSNKVTA PEETPEQLLT HALKLNKFDV ISDEFGEIIA 610 620 630 640 650 660 INDKEKTLFN YYRNNILHLF AVPSLIALHL FREKTTTVSK CQQLVAAFYP LFAKEWYLRE 670 680 690 700 710 720 LDEDYITRIL ANFVDQNLIE LDGDNIHITN TNDCLAKLDM LGKALNFTLQ RYAIVIGFIQ 730 740 750 760 770 780 TSNGIEKAEL ERESQVLAQR LGTLHGIKTP EFFDKKVLVS FIDNLRAQSL ITDGDAGLIG 790 800 810 SVQLCETYMH LKALLPARVW QSISDIVQGQ CK

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954246 Genomic DNA. Translation: CAI85246.1.
RefSeq	YP_338689.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3IF27.
Genome annotation databases
GeneID	3709901.
GenomeReviews	Gene locus PSHAa0142 in contig CR954246_GR.
KEGG	pha:PSHAa0142.
NMPDR	fig|326442.4.peg.227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAA0142-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEHT

Accession

Primary (citable) accession number: Q3IF27

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)