Q3IEY8 (PROB1_PSEHT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase 1
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase 1
Short name=GK 1

Gene names

Name:	proB1
Ordered Locus Names:	PSHAa0279

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas › Pseudoalteromonas haloplanktis

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Glutamate 5-kinase 1 HAMAP-Rule MF_00456		PRO_0000230055
Regions
	Domain	274 – 348	75	PUA
	Nucleotide binding	167 – 168	2	ATP By similarity
	Nucleotide binding	209 – 215	7	ATP By similarity
Sites
	Binding site	12	1	ATP By similarity
	Binding site	52	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	147	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3IEY8 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 71D26AC8C04EC235	FASTA	368	40,037
	10 20 30 40 50 60 MNKLNWRRIV LKVGSALIAP DQDGCRSRYI LTIAQFIVRC RARGIEVILV SSGSVAAGAH 70 80 90 100 110 120 LFPSDTARSV VMKKAMAAAG QTEMIAMWDR FFDFPSAQLL LTHGDLRDHE RYQSIRETVF 130 140 150 160 170 180 TLLEHGVLPI INENDAVTTD DLKVGDNDNL SAMVAAAADA DALLIFSDVD GLYDKNPNLH 190 200 210 220 230 240 DDAILLPEIK SIDDSIYAMA GCATSAVGTG GMKTKIEAAE KATSHGISTY IINGFKEETF 250 260 270 280 290 300 TRLLAGENPG TIFLPYEKPM QDSVHWMTHT ANEQGEVVVD GSFDKSLEGE TGCIRGDEIM 310 320 330 340 350 360 AVHGEFAIGD TILVRSEDGT RLAKATANYS SCLLSFIADN EQSEFSEKMQ DSIGPVISEK HIALLEKS

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TAC 125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954246 Genomic DNA. Translation: CAI85378.1.
RefSeq	YP_338821.1. NC_007481.1.
3D structure databases
ProteinModelPortal	Q3IEY8.
ModBase	Search...
Protein-protein interaction databases
STRING	326442.PSHAa0279.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI85378; CAI85378; PSHAa0279.
GeneID	3709886.
KEGG	pha:PSHAa0279.
PATRIC	32294091. VBIPseHal105694_0265.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	FDFPSAQ.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	PHAL326442:GJIU-283-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB1_PSEHT

Accession

Primary (citable) accession number: Q3IEY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program