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Q3IEA2

- SYI_PSEHT

UniProt

Q3IEA2 - SYI_PSEHT

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
    Binding sitei611 – 6111ATPUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPHAL326442:GJIU-925-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PSHAa0918
    OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
    Taxonomic identifieri326442 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
    ProteomesiUP000006843: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 942942Isoleucine--tRNA ligasePRO_0000098443Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi326442.PSHAa0918.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3IEA2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi608 – 6125"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3IEA2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKHTLNL PETPFPMRGN LAQREPKMLK AWYEDDLYGQ IRSAKKGKKT    50
    FILHDGPPYA NGDIHLGHSV NKILKDIIVK SKTLSDFDSP LVPGWDCHGL 100
    PIELMVEKKV GKPGVKVTAS EFREKCRAYA KKQVEGQKVD FKRLGVFADW 150
    DKPYLTMNFD FEANAIRVLG RIIEKGHLHK GAKPVHWCTD CGSALAEAEV 200
    EYQDKQSPAI DVRFIFNDQD AVVSAFDLAD GHKGTGKVGT VIWTTTPWTL 250
    PANRAVAVHA ELEYALVQVE DEGKQQRLIL GSELVKDAMD RFGFNQFHVL 300
    GYVKGAALEN LQVAHPFYDF SVPVIVAEHV TTDSGTGVVH TAPGHGQEDF 350
    VAGLEYKLEV ANPVGANGVY LPDTELFAGQ HVFKANASIV EVLKEHGALM 400
    HHHAITHSYP HCWRHKTPII FRATPQWFIS MDQANLRQDS LNEIKNTQWL 450
    PEWGESRIAN MVEGRPDWCI SRQRTWGVPI ALFADKDTGA IHPNTQELIE 500
    QAAQLVEKSG IQAWYDLDPA TLLGEEDAKQ YMKVQDTLDV WFDSGVSHAC 550
    VVDAREDLTG PADLYLEGSD QHRGWFMSSM MTSVAINGHA PYRQVLTHGF 600
    TVDENGRKMS KSLGNVISPQ NVMNKLGADI LRLWVASTDY TAEMTVSDEI 650
    FNRSADRYRR IRNTSRYLLA NLNGFDPKTD QVAVNDMVEL DKWIVGRAAQ 700
    LQTEILAAYD SYQMLLVTQK LMNFCTGELG SFYLDVIKDR QYTAKSDSHA 750
    RRSCQTALYH IAEAMTRWMA PIMSFTAQEI WEALPGERSD YVFTSTWYEG 800
    LQAPTNSQFS NDDWLEILTV RDEVNRLLEA ARKEEVIGAT LQATVNLYTG 850
    KALAAKLLAL GDELRFVFLT SAVNVTEVDS QPADTQTTEI DGLYISVAAT 900
    DAQKCERCWH YSADVGVEPA HPEICGRCVS NVDGEGEQRQ FA 942
    Length:942
    Mass (Da):105,542
    Last modified:November 8, 2005 - v1
    Checksum:i6485980DBC00E148
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954246 Genomic DNA. Translation: CAI85997.1.
    RefSeqiYP_339440.1. NC_007481.1.

    Genome annotation databases

    EnsemblBacteriaiCAI85997; CAI85997; PSHAa0918.
    GeneIDi3710090.
    KEGGipha:PSHAa0918.
    PATRICi32295363. VBIPseHal105694_0879.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR954246 Genomic DNA. Translation: CAI85997.1 .
    RefSeqi YP_339440.1. NC_007481.1.

    3D structure databases

    ProteinModelPortali Q3IEA2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 326442.PSHAa0918.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI85997 ; CAI85997 ; PSHAa0918 .
    GeneIDi 3710090.
    KEGGi pha:PSHAa0918.
    PATRICi 32295363. VBIPseHal105694_0879.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PHAL326442:GJIU-925-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TAC 125.

    Entry informationi

    Entry nameiSYI_PSEHT
    AccessioniPrimary (citable) accession number: Q3IEA2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3