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Q3IEA2

- SYI_PSEHT

UniProt

Q3IEA2 - SYI_PSEHT

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PSHAa0918
Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylate By similarity
Binding sitei611 – 6111ATP By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-925-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PSHAa0918
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098443Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi326442.PSHAa0918.

Structurei

3D structure databases

ProteinModelPortaliQ3IEA2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IEA2-1 [UniParc]FASTAAdd to Basket

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MSDYKHTLNL PETPFPMRGN LAQREPKMLK AWYEDDLYGQ IRSAKKGKKT    50
FILHDGPPYA NGDIHLGHSV NKILKDIIVK SKTLSDFDSP LVPGWDCHGL 100
PIELMVEKKV GKPGVKVTAS EFREKCRAYA KKQVEGQKVD FKRLGVFADW 150
DKPYLTMNFD FEANAIRVLG RIIEKGHLHK GAKPVHWCTD CGSALAEAEV 200
EYQDKQSPAI DVRFIFNDQD AVVSAFDLAD GHKGTGKVGT VIWTTTPWTL 250
PANRAVAVHA ELEYALVQVE DEGKQQRLIL GSELVKDAMD RFGFNQFHVL 300
GYVKGAALEN LQVAHPFYDF SVPVIVAEHV TTDSGTGVVH TAPGHGQEDF 350
VAGLEYKLEV ANPVGANGVY LPDTELFAGQ HVFKANASIV EVLKEHGALM 400
HHHAITHSYP HCWRHKTPII FRATPQWFIS MDQANLRQDS LNEIKNTQWL 450
PEWGESRIAN MVEGRPDWCI SRQRTWGVPI ALFADKDTGA IHPNTQELIE 500
QAAQLVEKSG IQAWYDLDPA TLLGEEDAKQ YMKVQDTLDV WFDSGVSHAC 550
VVDAREDLTG PADLYLEGSD QHRGWFMSSM MTSVAINGHA PYRQVLTHGF 600
TVDENGRKMS KSLGNVISPQ NVMNKLGADI LRLWVASTDY TAEMTVSDEI 650
FNRSADRYRR IRNTSRYLLA NLNGFDPKTD QVAVNDMVEL DKWIVGRAAQ 700
LQTEILAAYD SYQMLLVTQK LMNFCTGELG SFYLDVIKDR QYTAKSDSHA 750
RRSCQTALYH IAEAMTRWMA PIMSFTAQEI WEALPGERSD YVFTSTWYEG 800
LQAPTNSQFS NDDWLEILTV RDEVNRLLEA ARKEEVIGAT LQATVNLYTG 850
KALAAKLLAL GDELRFVFLT SAVNVTEVDS QPADTQTTEI DGLYISVAAT 900
DAQKCERCWH YSADVGVEPA HPEICGRCVS NVDGEGEQRQ FA 942
Length:942
Mass (Da):105,542
Last modified:November 8, 2005 - v1
Checksum:i6485980DBC00E148
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI85997.1.
RefSeqiYP_339440.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI85997; CAI85997; PSHAa0918.
GeneIDi3710090.
KEGGipha:PSHAa0918.
PATRICi32295363. VBIPseHal105694_0879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR954246 Genomic DNA. Translation: CAI85997.1 .
RefSeqi YP_339440.1. NC_007481.1.

3D structure databases

ProteinModelPortali Q3IEA2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326442.PSHAa0918.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI85997 ; CAI85997 ; PSHAa0918 .
GeneIDi 3710090.
KEGGi pha:PSHAa0918.
PATRICi 32295363. VBIPseHal105694_0879.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PHAL326442:GJIU-925-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiSYI_PSEHT
AccessioniPrimary (citable) accession number: Q3IEA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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