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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudoalteromonas haloplanktis (strain TAC 125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
Binding sitei611 – 6111ATPUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-925-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PSHAa0918
OrganismiPseudoalteromonas haloplanktis (strain TAC 125)
Taxonomic identifieri326442 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000006843 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942Isoleucine--tRNA ligasePRO_0000098443Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi326442.PSHAa0918.

Structurei

3D structure databases

ProteinModelPortaliQ3IEA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3IEA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PETPFPMRGN LAQREPKMLK AWYEDDLYGQ IRSAKKGKKT
60 70 80 90 100
FILHDGPPYA NGDIHLGHSV NKILKDIIVK SKTLSDFDSP LVPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKPGVKVTAS EFREKCRAYA KKQVEGQKVD FKRLGVFADW
160 170 180 190 200
DKPYLTMNFD FEANAIRVLG RIIEKGHLHK GAKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYQDKQSPAI DVRFIFNDQD AVVSAFDLAD GHKGTGKVGT VIWTTTPWTL
260 270 280 290 300
PANRAVAVHA ELEYALVQVE DEGKQQRLIL GSELVKDAMD RFGFNQFHVL
310 320 330 340 350
GYVKGAALEN LQVAHPFYDF SVPVIVAEHV TTDSGTGVVH TAPGHGQEDF
360 370 380 390 400
VAGLEYKLEV ANPVGANGVY LPDTELFAGQ HVFKANASIV EVLKEHGALM
410 420 430 440 450
HHHAITHSYP HCWRHKTPII FRATPQWFIS MDQANLRQDS LNEIKNTQWL
460 470 480 490 500
PEWGESRIAN MVEGRPDWCI SRQRTWGVPI ALFADKDTGA IHPNTQELIE
510 520 530 540 550
QAAQLVEKSG IQAWYDLDPA TLLGEEDAKQ YMKVQDTLDV WFDSGVSHAC
560 570 580 590 600
VVDAREDLTG PADLYLEGSD QHRGWFMSSM MTSVAINGHA PYRQVLTHGF
610 620 630 640 650
TVDENGRKMS KSLGNVISPQ NVMNKLGADI LRLWVASTDY TAEMTVSDEI
660 670 680 690 700
FNRSADRYRR IRNTSRYLLA NLNGFDPKTD QVAVNDMVEL DKWIVGRAAQ
710 720 730 740 750
LQTEILAAYD SYQMLLVTQK LMNFCTGELG SFYLDVIKDR QYTAKSDSHA
760 770 780 790 800
RRSCQTALYH IAEAMTRWMA PIMSFTAQEI WEALPGERSD YVFTSTWYEG
810 820 830 840 850
LQAPTNSQFS NDDWLEILTV RDEVNRLLEA ARKEEVIGAT LQATVNLYTG
860 870 880 890 900
KALAAKLLAL GDELRFVFLT SAVNVTEVDS QPADTQTTEI DGLYISVAAT
910 920 930 940
DAQKCERCWH YSADVGVEPA HPEICGRCVS NVDGEGEQRQ FA
Length:942
Mass (Da):105,542
Last modified:November 8, 2005 - v1
Checksum:i6485980DBC00E148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA. Translation: CAI85997.1.
RefSeqiWP_011327608.1. NC_007481.1.
YP_339440.1. NC_007481.1.

Genome annotation databases

EnsemblBacteriaiCAI85997; CAI85997; PSHAa0918.
GeneIDi3710090.
KEGGipha:PSHAa0918.
PATRICi32295363. VBIPseHal105694_0879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954246 Genomic DNA. Translation: CAI85997.1.
RefSeqiWP_011327608.1. NC_007481.1.
YP_339440.1. NC_007481.1.

3D structure databases

ProteinModelPortaliQ3IEA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326442.PSHAa0918.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI85997; CAI85997; PSHAa0918.
GeneIDi3710090.
KEGGipha:PSHAa0918.
PATRICi32295363. VBIPseHal105694_0879.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciPHAL326442:GJIU-925-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TAC 125.

Entry informationi

Entry nameiSYI_PSEHT
AccessioniPrimary (citable) accession number: Q3IEA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 8, 2005
Last modified: May 27, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.