Reviewed, UniProtKB/Swiss-Prot Q3ID04 (ILVD2_PSEHT)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	PSHAb0129

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000225409
Sites
	Metal binding	126	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3ID04-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: A00BF714F52BE565	FASTA	559	58,832
	10 20 30 40 50 60 MTSEKPRKYS QKIVDGSAQA PSRSMLRAVG FNDDDFKKSQ VGIASTWSMV TPCNMHINTL 70 80 90 100 110 120 AEEVGKGVDS AGAKSVIYNT ITVSDGISMG TEGMKYSLVS REVICDSIEA VSAGMGHDGI 130 140 150 160 170 180 IAIGGCDKNM PGCLMGLARL NRPSIFVYGG TILPGENHTD IVSVFEAVGS YAAGDIPITQ 190 200 210 220 230 240 LEHIEKTAIP GAGSCGGMYT ANTLASAIEA LGMSMPNSSA QNAISQNKKQ DCIDAGKAIV 250 260 270 280 290 300 YLLEHDIKPS DIMTKKAFEN AITLIITLGG STNAVLHLIA MADAVGVEVT LDDFVRIGDK 310 320 330 340 350 360 TPVIADLRPS GQYLMSELIE IGGIQPLMKR LLDAGMLHGD CMTVTGKTMA ENLADVADYP 370 380 390 400 410 420 AGQNIILPFD KPIKKDSHLV ILAGNLAPEG AVSKITGKEG LRFTGNAKVY DSEEQGFAAI 430 440 450 460 470 480 IDGQVVAGDV VVIRYEGPKG GPGMREMLSP TSAIMGKGLG NDVALITDGR FSGGSRGFVV 490 500 510 520 530 540 GHVTPEAYEG GAIALVENGD SITIDAQSRE MTLNITDEEM ASRKQRWQQP APKYTRGLLA 550 KYARTVSSAS TGAVTDKPS

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR954247 Genomic DNA. Translation: CAI89176.1.
RefSeq	YP_341622.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ID04.
Genome annotation databases
GeneID	3711126.
GenomeReviews	Gene locus PSHAb0129 in contig CR954247_GR.
KEGG	pha:PSHAb0129.
NMPDR	fig|326442.4.peg.3166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	Q3ID04.
Enzyme and pathway databases
BioCyc	PHAL326442:PSHAB0129-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_PSEHT

Accession

Primary (citable) accession number: Q3ID04

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)