Reviewed, UniProtKB/Swiss-Prot Q3IBW7 (RLMF_PSEHT)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase F
    EC=2.1.1.48
Alternative name(s):
    23S rRNA mA1618 methyltransferase
    rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	PSHAb0296

Organism

Pseudoalteromonas haloplanktis (strain TAC 125) [Complete proteome] [HAMAP]

Taxonomic identifier

326442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine. HAMAP MF_01848
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. METT10D/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349924

Sequences

	Sequence		Length	Mass (Da)
	Q3IBW7-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 6C81F555D8294349	FASTA	310	34,702
	10 20 30 40 50 60 MKASTSRAKL HPRNQHLNGY DLDLLTEQHP ALSAFIITTP AGTKSIDFSD NKAVKTLNQA 70 80 90 100 110 120 LLKAHYNVDF WDIPQHNLCP PIPGRVDYIH YLADLLADDN QTQIPTGRQV KVLDIGTGAN 130 140 150 160 170 180 LVYPLTGGHE YNWHFTGTDI DPAAIKVAKQ IAQFNNLKIT LKQQKNAANI FKGVINSKDL 190 200 210 220 230 240 YHLTLCNPPF HASSEDAVKG SERKWKNLGK APSSTLNFGG QNNELWCEGG EVQFISNMIK 250 260 270 280 290 300 ESAAVAEQVM WFTSLVSKKD NIAALEQELK QHPIAQYKIV DMAQGQKVSR FIAWSYFDKA 310 TREQICEELE

References

[1]

"Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125." Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N., Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C., Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C. , Rouy Z., Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A. Genome Res. 15:1325-1335(2005) [PubMed: 16169927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR954247 Genomic DNA. Translation: CAI89337.1. Different initiation.
RefSeq	YP_341783.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3711249.
GenomeReviews	Gene locus PSHAb0296 in contig CR954247_GR.
KEGG	pha:PSHAb0296.
NMPDR	fig|326442.4.peg.3209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3IBW7.
Family and domain databases
HAMAP	MF_01848. [Tree]
InterPro	IPR016909. S-AdoMet-dep_MeTrfase_YbiN_prd. IPR010286. SAM-Mtase_prd. [Graphical view]
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSEHT

Accession

Primary (citable) accession number: Q3IBW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)