Reviewed, UniProtKB/Swiss-Prot Q3I411 (G3O21_WHEAT)

Names and origin

Protein names

Recommended name:
    Gibberellin 3-beta-dioxygenase 2-1
    EC=1.14.11.15
Alternative name(s):
    Gibberellin 3 beta-hydroxylase 2-1
    Gibberellin 3-oxidase 2-1

Gene names

Name:

GA3ox2-1

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts the inactive gibbberellin precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1. Accepts also GA15, GA44, the 2,3-unsaturated GA5 and 2,3-dihydroGA9 as substrate. No activity with GA12, GA53, GA24, GA19 and GA25. Possesses also 2-beta-hydroxylase, 2,3-desaturase, 2,3-epoxidase and 13-hydroxylase activities.
Catalytic activity	Gibberellin 20 + 2-oxoglutarate + O(2) = gibberellin 1 + succinate + CO(2).
Cofactor	Ascorbate By similarity. Iron By similarity.
Tissue specificity	Expressed in internodes, nodes and the ear of the elongating stem.
Developmental stage	Highly expressed in the embryo and the surrounding maternal tissues, the pericarp and the integuments. Also found in the germinating grain.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily.
Biophysicochemical properties	Kinetic parameters: KM=1 µM for GA9 KM=2.02 µM for GA20

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gibberellin 3-beta-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Gibberellin 3-beta-dioxygenase 2-1		PRO_0000067317
Sites
	Active site	297	1	Potential
	Metal binding	229	1	Iron By similarity
	Metal binding	231	1	Iron By similarity
	Metal binding	287	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3I411-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 34930E368D91D3DD	FASTA	370	40,245
	10 20 30 40 50 60 MPTPSHLSKD PRYFDFRAAR RVPETHAWPG LHDHPVVDGS GAGGGPDAVP VVDMRDPCAA 70 80 90 100 110 120 EAVALAAQDW GAFLLEGHGV PLELLAGVEA AIGGMFALPA SEKMRAVRRP GDSCGYGSPP 130 140 150 160 170 180 ISSFFSKCMW SEGYTFSPAN LRSDLRKLWP KAGHDYRHFC AVMEEFHREM RALADKLLEL 190 200 210 220 230 240 FLVALGLTGE QVAAVESEHK IAETMTATMH LNWYPKCPDP KRALGLIAHT DSGFFTFVLQ 250 260 270 280 290 300 SLVPGLQLFR HGPDRWVTVP AVPGAMVVNV GDLFQILTNG RFHSVYHRAV VNRDSDRISL 310 320 330 340 350 360 GYFLGPPAHV KVAPLREALA GTPAAYRAVT WPEYMGVRKK AFTTGASALK MVAISTDNDA 370 ANDTDDLISS

References

[1]

"Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat." Appleford N.E., Evans D.J., Lenton J.R., Gaskin P., Croker S.J., Devos K.M., Phillips A.L., Hedden P. Planta 223:568-582(2006) [PubMed: 16160850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: cv. Maris Huntsman. Tissue: Scutellum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ118250 mRNA. Translation: AAZ94377.1.
3D structure databases
ModBase	Search...
Organism-specific databases
Gramene	Q3I411.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11644.
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3O21_WHEAT

Accession

Primary (citable) accession number: Q3I411

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 8, 2005
Last modified:	November 4, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)