Q3I409 (G3O23_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gibberellin 3-beta-dioxygenase 2-3
EC=1.14.11.15

Alternative name(s):
Gibberellin 3 beta-hydroxylase 2-3
Gibberellin 3-oxidase 2-3

Gene names

Name:

GA3ox2-3

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts the inactive gibbberellin precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1. Ref.1
Catalytic activity	Gibberellin 20 + 2-oxoglutarate + O2 = gibberellin 1 + succinate + CO2.
Cofactor	Ascorbate By similarity. Iron By similarity.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Molecular function	gibberellin 3-beta-dioxygenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Gibberellin 3-beta-dioxygenase 2-3		PRO_0000067319
Regions
	Domain	205 – 306	102	Fe2OG dioxygenase
Sites
	Active site	297	1	Potential
	Metal binding	229	1	Iron By similarity
	Metal binding	231	1	Iron By similarity
	Metal binding	287	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3I409 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: FC13E8D7908274F8	FASTA	369	40,326
	10 20 30 40 50 60 MPTPAHLSKD PHYFDFRAAR RVPETHAWPG LHDHPVVDGS GAGGEPDAVP VVDMRDPFAA 70 80 90 100 110 120 EAVGLAAQDW GAFLLVGHGV PLDLLVRVEA AIAGMFALPA SEKMRAVRRP GDSCGYGSPP 130 140 150 160 170 180 ISSFFSKCMW SEGYTFSPAN LRSDLRKLWP KAGHDYRHFC AVMEEFHREM RALADKLLEL 190 200 210 220 230 240 FLVALGLTGE QVAAVESEQK IAETMTATMH LNWYPKCPDP KRALGLIAHT DSGFFTFVLQ 250 260 270 280 290 300 SLVPGLQLFR HGPDRWVTVP AVPGAMVVNV GDLFQILTNG RFHSVYHRAV VNRESDRISL 310 320 330 340 350 360 GYFLGPPAHV KVAPLREALA GTPAAYRAVT WPEYMGVRKK AFTTGASALK MVAISTDDAA NDTDDLILS

References

[1]

"Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat." Appleford N.E., Evans D.J., Lenton J.R., Gaskin P., Croker S.J., Devos K.M., Phillips A.L., Hedden P. Planta 223:568-582(2006) [PubMed: 16160850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Maris Huntsman. Tissue: Scutellum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ118252 mRNA. Translation: AAZ94379.1.
3D structure databases
ProteinModelPortal	Q3I409.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q3I409.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11646.
BRENDA	1.14.11.15. 6500.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3O23_WHEAT

Accession

Primary (citable) accession number: Q3I409

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 8, 2005
Last modified:	September 21, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program