Reviewed, UniProtKB/Swiss-Prot Q3I354 (LUXS_CAMJJ)

Names and origin

Protein names

Recommended name:
    S-ribosylhomocysteine lyase
    EC=4.4.1.21
Alternative name(s):
    Autoinducer-2 production protein luxS
    AI-2 synthesis protein

Gene names

Name:	luxS
Ordered Locus Names:	CJJ81176_1213

Organism

Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) [Complete proteome] [HAMAP]

Taxonomic identifier

354242 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity.
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the luxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_0000297988
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	128	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3I354-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 91807C03A5D01ACB	FASTA	164	18,257
	10 20 30 40 50 60 MPLLDSFKVN HTKMPAPAVR LAKVMKTPKG DDISVFDLRF CVPNKDIMSE KGTHTLEHLF 70 80 90 100 110 120 AGFMRDHLNS DSVEIIDISP MGCRTGFYMS LIGTPDEKSV AKAWEEAMKD VLSVSDQSKI 130 140 150 160 PELNIYQCGT CAMHSLDEAK QIAQKVLNLG ISIMNNKELK LENA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Onset time as an objective parameter for evaluating AI-2 activity in the Vibrio harveyi bioluminescence assay." Chen C.-Y., Irwin P.L. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fouts D.E., Nelson K.E., Sebastian Y. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ143940 Genomic DNA. Translation: ABA26840.1. CP000538 Genomic DNA. Translation: EAQ72038.1.
RefSeq	YP_001000873.1.
3D structure databases
SMR	Q3I354. Positions 3-163.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3I354.
Genome annotation databases
GeneID	4683564.
GenomeReviews	Gene locus CJJ81176_1213 in contig CP000538_GR.
KEGG	cjj:CJJ81176_1213.
TIGR	CJJ81176_1213.
Phylogenomic databases
OMA	VRIAKTM.
Family and domain databases
HAMAP	MF_00091. [Tree]
InterPro	IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. LuxS. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

LUXS_CAMJJ

Accession

Primary (citable) accession number: Q3I354

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)