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Protein

Genome polyprotein

Gene
N/A
Organism
West Nile virus (WNV)
Status
Unreviewed-Annotation score: -Protein predictedi

Functioni

Peptide 2k: Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.SAAS annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.SAAS annotation
NTP + H2O = NDP + phosphate.SAAS annotation
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.SAAS annotation
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].SAAS annotation
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1556Charge relay system; for serine protease NS3 activityUniRule annotation1
Active sitei1580Charge relay system; for serine protease NS3 activityUniRule annotation1
Active sitei1640Charge relay system; for serine protease NS3 activityUniRule annotation1
Binding sitei2584S-adenosyl-L-methionineUniRule annotation1
Binding sitei2614S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei2615S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei2632S-adenosyl-L-methionineUniRule annotation1
Binding sitei2633S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei2659S-adenosyl-L-methionineUniRule annotation1
Binding sitei2660S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei2748S-adenosyl-L-methionineUniRule annotation1
Metal bindingi2968Zinc 1UniRule annotation1
Metal bindingi2972Zinc 1; via tele nitrogenUniRule annotation1
Metal bindingi2977Zinc 1UniRule annotation1
Metal bindingi2980Zinc 1UniRule annotation1
Metal bindingi3245Zinc 2; via tele nitrogenUniRule annotation1
Metal bindingi3261Zinc 2UniRule annotation1
Metal bindingi3380Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicaseSAAS annotation, Hydrolase, MethyltransferaseSAAS annotation, Nucleotidyltransferase, Protease, RNA-bindingSAAS annotation, RNA-directed RNA polymeraseSAAS annotation, Serine proteaseSAAS annotation, Transferase
Biological processActivation of host autophagy by virusSAAS annotation, Fusion of virus membrane with host endosomal membraneSAAS annotation, Fusion of virus membrane with host membrane, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT2 by virusSAAS annotation, Viral attachment to host cellSAAS annotation, Viral immunoevasion, Viral penetration into host cytoplasm, Viral RNA replicationSAAS annotation, Virus entry into host cell
LigandATP-binding, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyproteinSAAS annotation
OrganismiWest Nile virus (WNV)Imported
Taxonomic identifieri11082 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageFlaviviridaeFlavivirusJapanese encephalitis virus group
Virus hostiAedes [TaxID: 7158]
Amblyomma variegatum (Tropical bont tick) [TaxID: 34610]
Aves [TaxID: 8782]
Culex [TaxID: 53527]
Homo sapiens (Human) [TaxID: 9606]
Hyalomma marginatum [TaxID: 34627]
Mansonia uniformis [TaxID: 308735]
Mimomyia [TaxID: 308737]
Rhipicephalus [TaxID: 34630]
Proteomesi
  • UP000129813 Componenti: Genome

Subcellular locationi

  • Host endoplasmic reticulum membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Lumenal side SAAS annotation
  • Host nucleus SAAS annotation
  • Secreted SAAS annotation
  • Virion membrane SAAS annotation; Multi-pass membrane protein SAAS annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei44 – 66HelicalSequence analysisAdd BLAST23
Transmembranei107 – 126HelicalSequence analysisAdd BLAST20
Transmembranei250 – 269HelicalSequence analysisAdd BLAST20
Transmembranei276 – 292HelicalSequence analysisAdd BLAST17
Transmembranei743 – 764HelicalSequence analysisAdd BLAST22
Transmembranei1179 – 1197HelicalSequence analysisAdd BLAST19
Transmembranei1217 – 1238HelicalSequence analysisAdd BLAST22
Transmembranei1245 – 1261HelicalSequence analysisAdd BLAST17
Transmembranei1281 – 1305HelicalSequence analysisAdd BLAST25
Transmembranei1312 – 1330HelicalSequence analysisAdd BLAST19
Transmembranei1342 – 1366HelicalSequence analysisAdd BLAST25
Transmembranei1378 – 1396HelicalSequence analysisAdd BLAST19
Transmembranei1402 – 1420HelicalSequence analysisAdd BLAST19
Transmembranei1472 – 1498HelicalSequence analysisAdd BLAST27
Transmembranei2174 – 2195HelicalSequence analysisAdd BLAST22
Transmembranei2204 – 2222HelicalSequence analysisAdd BLAST19
Transmembranei2228 – 2245HelicalSequence analysisAdd BLAST18
Transmembranei2257 – 2279HelicalSequence analysisAdd BLAST23
Transmembranei2312 – 2329HelicalSequence analysisAdd BLAST18
Transmembranei2350 – 2373HelicalSequence analysisAdd BLAST24
Transmembranei2379 – 2398HelicalSequence analysisAdd BLAST20
Transmembranei2446 – 2465HelicalSequence analysisAdd BLAST20

GO - Cellular componenti

Keywords - Cellular componenti

Capsid proteinSAAS annotation, Host endoplasmic reticulumSAAS annotation, Host membraneSAAS annotation, Host nucleusSAAS annotation, Membrane, SecretedSAAS annotation, Virion

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi293 ↔ 320UniRule annotation
Disulfide bondi350 ↔ 406UniRule annotation
Disulfide bondi364 ↔ 395UniRule annotation
Disulfide bondi382 ↔ 411UniRule annotation
Disulfide bondi480 ↔ 578UniRule annotation
Disulfide bondi595 ↔ 626UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotationSAAS annotation

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ3I0Y8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3I0Y8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1375 – 1506FLAVIVIRUS_NS2BInterPro annotationAdd BLAST132
Domaini1506 – 1683Peptidase S7InterPro annotationAdd BLAST178
Domaini1686 – 1842Helicase ATP-bindingInterPro annotationAdd BLAST157
Domaini1853 – 2018Helicase C-terminalInterPro annotationAdd BLAST166
Domaini2529 – 2794MRNA cap 0-1 NS5-type MTInterPro annotationAdd BLAST266
Domaini3058 – 3210RdRp catalyticInterPro annotationAdd BLAST153

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysisSAAS annotation

Family and domain databases

CDDicd12149 Flavi_E_C, 1 hit
Gene3Di1.10.10.930, 1 hit
1.10.8.970, 1 hit
1.20.1280.260, 1 hit
2.60.260.50, 1 hit
2.60.40.350, 1 hit
2.60.98.10, 1 hit
InterProiView protein in InterPro
IPR011492 DEAD_Flavivir
IPR000069 Env_glycoprot_M_flavivir
IPR038302 Env_glycoprot_M_sf_flavivir
IPR001122 Flavi_capsidC
IPR037172 Flavi_capsidC_sf
IPR027287 Flavi_E_Ig-like
IPR026470 Flavi_E_Stem/Anchor_dom
IPR038345 Flavi_E_Stem/Anchor_dom_sf
IPR001157 Flavi_NS1
IPR000752 Flavi_NS2A
IPR000487 Flavi_NS2B
IPR000404 Flavi_NS4A
IPR001528 Flavi_NS4B
IPR002535 Flavi_propep
IPR038688 Flavi_propep_sf
IPR000336 Flavivir/Alphavir_Ig-like_sf
IPR001850 Flavivirus_NS3_S7
IPR014412 Gen_Poly_FLV
IPR011998 Glycoprot_cen/dimer
IPR036253 Glycoprot_cen/dimer_sf
IPR038055 Glycoprot_E_dimer_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR014756 Ig_E-set
IPR026490 mRNA_cap_0/1_MeTrfase
IPR027417 P-loop_NTPase
IPR009003 Peptidase_S1_PA
IPR000208 RNA-dir_pol_flavivirus
IPR007094 RNA-dir_pol_PSvirus
IPR002877 rRNA_MeTrfase_FtsJ_dom
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01003 Flavi_capsid, 1 hit
PF07652 Flavi_DEAD, 1 hit
PF02832 Flavi_glycop_C, 1 hit
PF00869 Flavi_glycoprot, 1 hit
PF01004 Flavi_M, 1 hit
PF00948 Flavi_NS1, 1 hit
PF01005 Flavi_NS2A, 1 hit
PF01002 Flavi_NS2B, 1 hit
PF01350 Flavi_NS4A, 1 hit
PF01349 Flavi_NS4B, 1 hit
PF00972 Flavi_NS5, 1 hit
PF01570 Flavi_propep, 1 hit
PF01728 FtsJ, 1 hit
PF00949 Peptidase_S7, 1 hit
PIRSFiPIRSF003817 Gen_Poly_FLV, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF101257 SSF101257, 1 hit
SSF50494 SSF50494, 1 hit
SSF52540 SSF52540, 2 hits
SSF53335 SSF53335, 1 hit
SSF56983 SSF56983, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR04240 flavi_E_stem, 1 hit
PROSITEiView protein in PROSITE
PS51527 FLAVIVIRUS_NS2B, 1 hit
PS51528 FLAVIVIRUS_NS3PRO, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50507 RDRP_SSRNA_POS, 1 hit
PS51591 RNA_CAP01_NS5_MT, 1 hit

Sequencei

Sequence statusi: Complete.

Q3I0Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKPGGPGK SRAVNMLKRG MPRVLSLIGL KRAMLSLIDG KGPIRFVLAL
60 70 80 90 100
LAFFRFTAIA PTRAVLDRWR GVNKQTAMKH LLSFKKELGT LTSAINRRSS
110 120 130 140 150
KQKKRGGKTG IAVMIGLIAS VGAVTLSNFQ GKVMMTVNAT DVTDVITIPT
160 170 180 190 200
AAGKNLCIVR AMDVGYMCDD TITYECPVLS AGNDPEDIDC WCTKSAVYVR
210 220 230 240 250
YGRCTKTRHS RRSRRSLTVQ THGESTLANK KGAWMDSTKA TRYLVKTESW
260 270 280 290 300
ILRNPGYALV AAVIGWMLGS NTMQRVVFVV LLLLVAPAYS FNCLGMSNRD
310 320 330 340 350
FLEGVSGATW VDLVLEGDSC VTIMSKDKPT IDVKMMNMEA ANLAEVRSYC
360 370 380 390 400
YLATVSDLST KAACPTMGEA HNDKRADPAF VCRQGVVDRG WGNGCGLFGK
410 420 430 440 450
GSIDTCAKFA CSTKAIGRTI LKENIKYEVA IFVHGPTTVE SHGNYSTQVG
460 470 480 490 500
ATQAGRFSIT PAAPSYTLKL GEYGEVTVDC EPRSGIDTNA YYVMTVGTKT
510 520 530 540 550
FLVHREWFMD LNLPWSSAGS TVWRNRETLM EFEEPHATKQ SVIALGSQEG
560 570 580 590 600
ALHQALAGAI PVEFSSNTVK LTSGHLKCRV KMEKLQLKGT TYGVCSKAFK
610 620 630 640 650
FLGTPADTGH GTVVLELQYT GTDGPCKVPI SSVASLNDLT PVGRLVTVNP
660 670 680 690 700
FVSVATANAK VLIELEPPFG DSYIVVGRGE QQINHHWHKS GSSIGKAFTT
710 720 730 740 750
TLKGAQRLAA LGDTAWDFGS VGGVFTSVGK AVHQVFGGAF RSLFGGMSWI
760 770 780 790 800
TQGLLGALLL WMGINARDRS IALTFLAVGG VLLFLSVNVH ADTGCAIDIS
810 820 830 840 850
RQELRCGSGV FIHNDVEAWM DRYKYYPETP QGLAKIIQKA HKEGVCGLRS
860 870 880 890 900
VSRLEHQMWE AVKDELNTLL KENGVDLSVV VEKQEGMYKS APKRLTATTE
910 920 930 940 950
KLEIGWKAWG KSILFAPELA NNTFVVDGPE TKECPTQNRA WNSLEVEDFG
960 970 980 990 1000
FGLTSTRMFL KVRESNTTEC DSKIIGTAVK NNLAIHSDLS YWIESRLNDT
1010 1020 1030 1040 1050
WKLERAVLGE VKSCTWPETH TLWGDGILES DLIIPVTLAG PRSNHNRRPG
1060 1070 1080 1090 1100
YKTQNQGPWD EGRVEIDFDY CPGTTVTLSE SCGHRGPATR TTTESGKLIT
1110 1120 1130 1140 1150
DWCCRSCTLP PLRYQTDSGC WYGMEIRPQR HDEKTLVQSQ VNAYNADMID
1160 1170 1180 1190 1200
PFQLGLLVVF LATQEVLRKR WTAKISMPAI LIALLVLVFG GITYTDVLRY
1210 1220 1230 1240 1250
VILVGAAFAE SNSGGDVVHL ALMATFKIQP VFMVASFLKA RWTNQENILL
1260 1270 1280 1290 1300
MLAAVFFQMA YHDARQILLW EIPDVLNSLA VAWMILRAIT FTTTSNVVVP
1310 1320 1330 1340 1350
LLALLTPGLR CLNLDVYRIL LLMVGIGSLI REKRSAAAKK KGASLLCLAL
1360 1370 1380 1390 1400
ASTGLFNPMI LAAGLIACDP NRKRGWPATE VMTAVGLMFA IVGGLAELDI
1410 1420 1430 1440 1450
DSMAIPMTIA GLMFAAFVIS GKSTDMWIER TADISWESDA EITGSSERVD
1460 1470 1480 1490 1500
VRLDDDGDFQ LMNDPGAPWK IWMLRMVCLA ISAYTPWAIL PSVVGFWITL
1510 1520 1530 1540 1550
QYTKRGGVLW DTPSPKEYKK GDTTTGVYRI MTRGLLGSYQ AGAGVMVEGV
1560 1570 1580 1590 1600
FHTLWHTTKG AALMSGEGRL DPYWGSVKED RLCYGGPWKL QHKWNGQDEV
1610 1620 1630 1640 1650
QMIVVEPGKN VKNVQTKPGV FKTPEGEIGA VTLDFPTGTS GSPIVDKNGD
1660 1670 1680 1690 1700
VIGLYGNGVI MPNGSYISAI VQGERMDEPI PAGFEPEMLR KKQITVLDLH
1710 1720 1730 1740 1750
PGAGKTRRIL PQIIKEAINR RLRTAVLAPT RVVAAEMAEA LRGLPIRYQT
1760 1770 1780 1790 1800
SAVPREHNGN EIVDVMCHAT LTHRLMSPHR VPNYNLFVMD EAHFTDPASI
1810 1820 1830 1840 1850
AARGYISTKV ELGEAAAIFM TATPPGTSDP FPESNSPISD LQTEIPDRAW
1860 1870 1880 1890 1900
NSGYEWITEY TGKTVWFVPS VKMGNEIALC LQRAGKKVVQ LNRKSYETEY
1910 1920 1930 1940 1950
PKCKNDDWDF VITTDISEMG ANFKASRVID SRKSVKPTII TEGEGRVILG
1960 1970 1980 1990 2000
EPSAVTAASA AQRRGRIGRN PSQVGDEYCY GGHTNEDDSN FAHWTEARIM
2010 2020 2030 2040 2050
LDNINMPNGL IAQFYQPERE KVYTMDGEYR LRGEERKNFL ELLRTADLPV
2060 2070 2080 2090 2100
WLAYKVAAAG VSYHDRRWCF DGPRTNTILE DNNEVEVITK LGERKILRPR
2110 2120 2130 2140 2150
WIDARVYSDH QALKAFKDFA SGKRSQIGLI EVLGKMPEHF MGKTWEALDT
2160 2170 2180 2190 2200
MYVVATAEKG GRAHRMALEE LPDALQTIAL IALLSVMTMG VFFLLMQRKG
2210 2220 2230 2240 2250
IGKIGLGGAV LGVATFFCWM AEVPGTKIAG MLLLSLLLMI VLIPEPEKQR
2260 2270 2280 2290 2300
SQTDNQLAVF LICVMTLVSA VAANEMGWLD KTKSDISSLF GQRIEVKENF
2310 2320 2330 2340 2350
SMGEFLLDLR PATAWSLYAV TTAVLTPLLK HLITSDYINT SLTSINVQAS
2360 2370 2380 2390 2400
ALFTLSRGFP FVDVGVSALL LAAGCWGQVT LTVTVTAATL LFCHYAYMVP
2410 2420 2430 2440 2450
GWQAEAMRSA QRRTAAGIMK NAVVDGIVAT DVPELERTTP IMQKKVGQIM
2460 2470 2480 2490 2500
LILVSLAAVV VNPSVKTVRE AGILITAAAV TLWENGASSV WNATTAIGLC
2510 2520 2530 2540 2550
HIMRGGWLSC LSITWTLIKN MEKPGLKRGG AKGRTLGEVW KERLNQMTKE
2560 2570 2580 2590 2600
EFTRYRKEAI IEVDRSAAKH ARKEGNVTGG HPVSRGTAKL RWLVERRFLE
2610 2620 2630 2640 2650
PVGKVIDLGC GRGGWCYYMA TQKRVQEVRG YTKGGPGHEE PQLVQSYGWN
2660 2670 2680 2690 2700
IVTMKSGVDV FYRPSECCDT LLCDIGESSS SAEVEEHRTI RVLEMVEDWL
2710 2720 2730 2740 2750
HRGPREFCVK VLCPYMPKVI EKMELLQRRY GGGLVRNPLS RNSTHEMYWV
2760 2770 2780 2790 2800
SRASGNVVHS VNMTSQVLLG RMEKRTWKGP QYEEDVNLGS GTRAVGKPLL
2810 2820 2830 2840 2850
NSDTSKIKNR IERLRREYSS TWHHDENHPY RTWNYHGSYD VKPTGSASSL
2860 2870 2880 2890 2900
VNGVVRLLSK PWDTITNVTT MAMTDTTPFG QQRVFKEKVD TKAPEPPEGV
2910 2920 2930 2940 2950
KYVLNETTNW LWAFLAREKR PRMCSREEFI RKVNSNAALG AMFEEQNQWR
2960 2970 2980 2990 3000
SAREAVEDPK FWEMVDEERX AHLRGECHTC IYNMMGKREK KPGEFGKAKG
3010 3020 3030 3040 3050
SRAIWFMWLG ARFLEFEALG FLNEDHWLGR KNSGGGVEGL GLQKLGYILR
3060 3070 3080 3090 3100
EVGTRPGGKI YADDTAGWDT RITRADLENE AKVLELLDGE HRRLARAIIE
3110 3120 3130 3140 3150
LTYRHKVVKV MRPAADGRTV MDVISREDQR GSGQVVTYAL NTFTNLAVQL
3160 3170 3180 3190 3200
VRMMEGEGVI GPDDVEKLTK GKGPKVRTWL FENGEERLSR MAVSGDDCVV
3210 3220 3230 3240 3250
KPLDDRFATS LHFLNAMSKV RKDIQEWKPS TGWYDWQQVP FCSNHFTELI
3260 3270 3280 3290 3300
MKDGRTLVVP CRGQDELVGR ARISPGAGWN VRDTACLAKS YAQMWLLLYF
3310 3320 3330 3340 3350
HRRDLRLMAN AICSAVPVNW VPTGRTTWSI HAGGEWMTTE DMLEVWNRVW
3360 3370 3380 3390 3400
IEENEWMEDK TPVEKWSDVP YSGKREDIWC GSLIGTRARA TWAENIQVAI
3410 3420 3430
NQVRAIIGDE KYVDYMSSLK RYEDTTLVED TVL
Length:3,433
Mass (Da):381,175
Last modified:November 8, 2005 - v1
Checksum:i156BA17F434DF705
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ164202 Genomic RNA Translation: ABA54591.1

Similar proteinsi

Entry informationi

Entry nameiQ3I0Y8_WNV
AccessioniPrimary (citable) accession number: Q3I0Y8
Entry historyiIntegrated into UniProtKB/TrEMBL: November 8, 2005
Last sequence update: November 8, 2005
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health