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Q3HZ71

- Q3HZ71_STREE

UniProt

Q3HZ71 - Q3HZ71_STREE

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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Streptococcus pneumoniae
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATPUniRule annotation
Binding sitei51 – 511ATPUniRule annotation
Binding sitei78 – 781ATPUniRule annotation
Binding sitei344 – 3441ATPUniRule annotation
Metal bindingi433 – 4331Magnesium 1; catalyticUniRule annotation
Sitei458 – 4581Interaction with DNAUniRule annotation
Sitei461 – 4611Interaction with DNAUniRule annotation
Metal bindingi506 – 5061Magnesium 1; catalyticUniRule annotation
Metal bindingi506 – 5061Magnesium 2UniRule annotation
Metal bindingi508 – 5081Magnesium 2UniRule annotation
Sitei513 – 5131Interaction with DNAUniRule annotation
Sitei629 – 6291Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi118 – 1247ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-HAMAP
  2. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, DNA-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotationImported
OrganismiStreptococcus pneumoniaeImported
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4I3HX-ray3.70A/B1-647[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini427 – 541115ToprimUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family.UniRule annotation
Belongs to the type II topoisomerase family. ParE type 2 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation
Contains Toprim domain.SAAS annotation

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00939. ParE_type2.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01058. parE_Gpos. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3HZ71-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKKEININN YNDDAIQVLE GLDAVRKRPG MYIGSTDGAG LHHLVWEIVD
60 70 80 90 100
NAVDEALSGF GDRIDVTINK DGSLTVQDHG RGMPTGMHAM GIPTVEVIFT
110 120 130 140 150
ILHAGGKFGQ GGYKTSGGLH GVGSSVVNAL SSWLEVEITR DGAVYKQRFE
160 170 180 190 200
NGGKPVTTLK KIGTAPKSKT GTKVTFMPDA TIFSTTDFKY NTISERLNES
210 220 230 240 250
AFLLKNVTLS LTDKRTDEAI EFHYENGVQD FVSYLNEDKE ILTPVLYFEG
260 270 280 290 300
EDNGFQVEVA LQYNDGFSDN ILSFVNNVRT KDGGTHETGL KSAITKVMND
310 320 330 340 350
YARKTGLLKE KDKNLEGSDY REGLAAVLSI LVPEEHLQFE GQTKDKLGSP
360 370 380 390 400
LARPVVDGIV ADKLTFFLME NGELASNLIR KAIKARDARE AARKARDESR
410 420 430 440 450
NGKKNKKDKG LLSGKLTPAQ SKNPAKNELY LVEGDSAGGS AKQGRDRKFQ
460 470 480 490 500
AILPLRGKVI NTAKAKMADI LKNEEINTMI YTIGAGVGAD FSIEDANYDK
510 520 530 540 550
IIIMTDADTD GAHIQTLLLT FFYRYMRPLV EAGHVYIALP PLYKMSKGKG
560 570 580 590 600
KKEEVAYAWT DGELEELRKQ FGKGATLQRY KGLGEMNADQ LWETTMNPET
610 620 630 640
RTLIRVTIED LARAERRVNV LMGDKVEPRR KWIEDNVKFT LEEATVF
Length:647
Mass (Da):71,649
Last modified:November 8, 2005 - v1
Checksum:iDB5CF701D5F80AB4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ175245 Genomic DNA. Translation: AAZ93046.1.
DQ175255 Genomic DNA. Translation: AAZ93056.1.
DQ175258 Genomic DNA. Translation: AAZ93059.1.
DQ175263 Genomic DNA. Translation: AAZ93064.1.
DQ175273 Genomic DNA. Translation: AAZ93074.1.
DQ175279 Genomic DNA. Translation: AAZ93080.1.
DQ175285 Genomic DNA. Translation: AAZ93086.1.
DQ175288 Genomic DNA. Translation: AAZ93089.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ175245 Genomic DNA. Translation: AAZ93046.1 .
DQ175255 Genomic DNA. Translation: AAZ93056.1 .
DQ175258 Genomic DNA. Translation: AAZ93059.1 .
DQ175263 Genomic DNA. Translation: AAZ93064.1 .
DQ175273 Genomic DNA. Translation: AAZ93074.1 .
DQ175279 Genomic DNA. Translation: AAZ93080.1 .
DQ175285 Genomic DNA. Translation: AAZ93086.1 .
DQ175288 Genomic DNA. Translation: AAZ93089.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4I3H X-ray 3.70 A/B 1-647 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPi MF_00939. ParE_type2.
InterProi IPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR005740. ParE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01058. parE_Gpos. 1 hit.
PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular evolution perspectives on intraspecific lateral DNA transfer of topoisomerase and gyrase loci in Streptococcus pneumoniae, with implications for fluoroquinolone resistance development and spread."
    Stanhope M.J., Walsh S.L., Becker J.A., Italia M.J., Ingraham K.A., Gwynn M.N., Mathie T., Poupard J.A., Miller L.A., Brown J.R., Amrine-Madsen H.
    Antimicrob. Agents Chemother. 49:4315-4326(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 0.15GR00SImported, 0.68Oh92SImported, 128.2Ca00SImported, 292.1AT96SImported, 543.1Ca99SImported, 588.1JA99SImported, 672.1US00SImported and 68.1SW99SImported.
  2. "Structure of an 'open' clamp type II topoisomerase-DNA complex provides a mechanism for DNA capture and transport."
    Laponogov I., Veselkov D.A., Crevel I.M., Pan X.S., Fisher L.M., Sanderson M.R.
    Nucleic Acids Res. 41:9911-9923(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.70 ANGSTROMS).

Entry informationi

Entry nameiQ3HZ71_STREE
AccessioniPrimary (citable) accession number: Q3HZ71
Entry historyi
Integrated into UniProtKB/TrEMBL: November 8, 2005
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3