Q3HWU8 (Q3HWU8_PSEPU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Esterase EMBL ABA39859.1
Organism	Pseudomonas putida (Arthrobacter siderocapsulatus) EMBL ABA39859.1
Taxonomic identifier	303 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 1ZOI
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	97	1	Nucleophile PDB 1ZOI
	Active site	256	1	Proton donor/acceptor PDB 1ZOI
	Site	31	1	Transition state stabilizer; via amide nitrogen PDB 1ZOI
	Site	98	1	Transition state stabilizer; via amide nitrogen PDB 1ZOI
	Site	122	1	Important for catalytic activity; via carbonyl oxygen PDB 1ZOI
	Site	227	1	Important for catalytic activity PDB 1ZOI

Sequences

	Sequence		Length	Mass (Da)
	Q3HWU8 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 3C974EFF670AB354	FASTA	276	30,164
	10 20 30 40 50 60 MSYVTTKDGV QIFYKDWGPR DAPVIHFHHG WPLSADDWDA QLLFFLAHGY RVVAHDRRGH 70 80 90 100 110 120 GRSSQVWDGH DMDHYADDVA AVVAHLGIQG AVHVGHSTGG GEVVRYMARH PEDKVAKAVL 130 140 150 160 170 180 IAAVPPLMVQ TPGNPGGLPK SVFDGFQAQV ASNRAQFYRD VPAGPFYGYN RPGVEASEGI 190 200 210 220 230 240 IGNWWRQGMI GSAKAHYDGI VAFSQTDFTE DLKGIQQPVL VMHGDDDQIV PYENSGVLSA 250 260 270 KLLPNGALKT YKGYPHGMPT THADVINADL LAFIRS

References

[1]	"Stereoselective esterase from Pseudomonas putida IFO12996 reveals alpha/beta hydrolase folds for D-beta-acetylthioisobutyric acid synthesis." Elmi F., Lee H.T., Huang J.Y., Hsieh Y.C., Wang Y.L., Chen Y.J., Shaw S.Y., Chen C.J. J. Bacteriol. 187:8470-8476(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: IFO12996 EMBL ABA39859.1.
[2]	"Enzymatic resolution of methyl DL-beta-acetylthioisobutyrate and DL-beta-acetylthioisobutyramide using a stereoselective esterase from Pseudomonas putida IFO12996." Shaw S.-Y., Chen Y.-J., Ou J.-J., Ho L. J. Mol. Catal., B Enzym. 38:163-170(2006) Cited for: NUCLEOTIDE SEQUENCE. Strain: IFO12996 EMBL ABA39859.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DQ187948 Genomic DNA. Translation: ABA39859.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZOI	X-ray	1.60	A/B/C	1-276	[»]

ProteinModelPortal

Q3HWU8.

SMR

Q3HWU8. Positions 2-276.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
[Graphical view]

PRINTS

PR00111. ABHYDROLASE.

ProtoNet

Search...

Other

EvolutionaryTrace

Q3HWU8.

Entry information

Entry name

Q3HWU8_PSEPU

Accession

Primary (citable) accession number: Q3HWU8

Entry history

Integrated into UniProtKB/TrEMBL:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	September 5, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)