Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Odontogenic ameloblast-associated protein

Gene

Odam

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Tooth-associated epithelia protein that probably plays a role in odontogenesis, the complex process that results in the initiation and generation of the tooth. May be incorporated in the enamel matrix at the end of mineralization process. Involved in the induction of RHOA activity via interaction with ARHGEF and expression of downstream factors such as ROCK. Plays a role in attachment of the junctional epithelium to the tooth surface.By similarity

GO - Biological processi

  • biomineral tissue development Source: UniProtKB-KW
  • odontogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Biomineralization

Names & Taxonomyi

Protein namesi
Recommended name:
Odontogenic ameloblast-associated protein
Alternative name(s):
Apin
Gene namesi
Name:Odam
Synonyms:Apin
ORF Names:EO-009
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1561883. Odam.

Subcellular locationi

  • Secreted 1 Publication
  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence analysisAdd
BLAST
Chaini16 – 278263Odontogenic ameloblast-associated proteinPRO_5000140667Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161O-linked (GalNAc...)Sequence analysis
Glycosylationi120 – 1201O-linked (GalNAc...)Sequence analysis
Glycosylationi240 – 2401O-linked (GalNAc...)Sequence analysis
Glycosylationi243 – 2431O-linked (GalNAc...)Sequence analysis
Glycosylationi249 – 2491O-linked (GalNAc...)Sequence analysis
Glycosylationi254 – 2541O-linked (GalNAc...)Sequence analysis

Post-translational modificationi

O-glycosylated.Curated

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3HS83.
PRIDEiQ3HS83.

Expressioni

Tissue specificityi

Highly expressed in tooth-associated epithelia. In tooth, it is only detected in the ameloblast layer of the enamel organ, starting at post-secretory transition and extending throughout the maturation stage. Also detected in epithelial cells of the gingiva which bind it to the tooth surface (junctional epithelium) (at protein level). Predominantly expressed in mandible, but also expressed at weak level in nasal and salivary glands, and at much lower level in epididymis.3 Publications

Developmental stagei

Specifically expressed in ameloblasts during maturation stages. Not expressed in pre-ameloblasts, weakly expressed in secretory ameloblasts, and strongly expressed in maturation-stage ameloblasts as well as in the junctional epithelium attached to the enamel of erupted molars.2 Publications

Interactioni

Subunit structurei

Interacts (via C-terminus) with ARHGEF5.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035991.

Structurei

3D structure databases

ProteinModelPortaliQ3HS83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Interaction with ARHGEF5By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi62 – 198137Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the ODAM family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IV9J. Eukaryota.
ENOG411182C. LUCA.
HOGENOMiHOG000115257.
HOVERGENiHBG096669.
InParanoidiQ3HS83.
PhylomeDBiQ3HS83.

Family and domain databases

InterProiIPR026802. Odam.
[Graphical view]
PANTHERiPTHR16237. PTHR16237. 1 hit.
PfamiPF15424. ODAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3HS83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIIILLGLI GATSSAPLIT QRLLSASNSH ELLLNLNNGQ LLPLQFQSAF
60 70 80 90 100
NSWIPPFPGL LQQQQQQAQV SGHPQFPLST LESFAGLFPN QIPFSRQVGF
110 120 130 140 150
AQGGQAGQPD FSQQQTPSQT QQASPMSYVV PVKVPQDQTQ MFQYYPVYML
160 170 180 190 200
LPWEQPQQTV TSSPQQTGQQ LYEEQIPFYN QFGFVPQQAE PGVPGGQQHL
210 220 230 240 250
VLDSFVGTAP ETPGMPAVEG PLYPQKEPIG FKQDNVGVST PSTSPKPDTG
260 270
NFFTSEINPT IAPLLPEQKV NADSLREP
Length:278
Mass (Da):30,443
Last modified:November 8, 2005 - v1
Checksum:i572C784AB4BE0B4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ198380 mRNA. Translation: ABA54404.1.
RefSeqiNP_001037739.1. NM_001044274.1.
UniGeneiRn.65661.

Genome annotation databases

GeneIDi641555.
KEGGirno:641555.
UCSCiRGD:1561883. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ198380 mRNA. Translation: ABA54404.1.
RefSeqiNP_001037739.1. NM_001044274.1.
UniGeneiRn.65661.

3D structure databases

ProteinModelPortaliQ3HS83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035991.

Proteomic databases

PaxDbiQ3HS83.
PRIDEiQ3HS83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi641555.
KEGGirno:641555.
UCSCiRGD:1561883. rat.

Organism-specific databases

CTDi54959.
RGDi1561883. Odam.

Phylogenomic databases

eggNOGiENOG410IV9J. Eukaryota.
ENOG411182C. LUCA.
HOGENOMiHOG000115257.
HOVERGENiHBG096669.
InParanoidiQ3HS83.
PhylomeDBiQ3HS83.

Miscellaneous databases

NextBioi714715.
PROiQ3HS83.

Family and domain databases

InterProiIPR026802. Odam.
[Graphical view]
PANTHERiPTHR16237. PTHR16237. 1 hit.
PfamiPF15424. ODAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of secreted and membrane proteins in the rat incisor enamel organ using a signal-trap screening approach."
    Moffatt P., Smith C.E., Sooknanan R., St Arnaud R., Nanci A.
    Eur. J. Oral Sci. 114:139-146(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "The amyloid protein APin is highly expressed during enamel mineralization and maturation in rat incisors."
    Park J.-C., Park J.-T., Son H.-H., Kim H.-J., Jeong M.-J., Lee C.-S., Dey R., Cho M.-I.
    Eur. J. Oral Sci. 115:153-160(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  3. "Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia."
    Moffatt P., Smith C.E., St Arnaud R., Nanci A.
    J. Cell. Biochem. 103:941-956(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, PROBABLE GLYCOSYLATION.
  4. "Odontogenic ameloblast-associated protein (ODAM) Mediates Junctional Epithelium Attachment to Tooth via Integrin-ODAM-Rho guanine nucleotide exchange factor 5 (ARHGEF5)-Ras homolog gene family member A (RhoA) Signaling."
    Lee H.K., Ji S., Park S.J., Choung H.W., Choi Y., Lee H.J., Park S.Y., Park J.C.
    J. Biol. Chem. 290:14740-14753(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiODAM_RAT
AccessioniPrimary (citable) accession number: Q3HS83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 8, 2005
Last modified: May 11, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.