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Protein

Protein inscuteable homolog

Gene

Insc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. Involved in spindle orientation during mitosis (PubMed:16301171, PubMed:21816348). May regulate cell proliferation and differentiation in the developing nervous system (PubMed:16301171). May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium (PubMed:16094321).3 Publications

GO - Biological processi

  • establishment of mitotic spindle orientation Source: MGI
  • lung epithelial cell differentiation Source: MGI
  • nervous system development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein inscuteable homolog
Alternative name(s):
Minsc
Gene namesi
Name:Insc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1917942. Insc.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: MGI
  • cell cortex Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi74S → E: Abolishes interaction with GPSM2. 1 Publication1
Mutagenesisi78W → E: Abolishes interaction with GPSM2. 1 Publication1
Mutagenesisi79M → E: Abolishes interaction with GPSM2. 1 Publication1
Mutagenesisi82L → E: Abolishes interaction with GPSM2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002524061 – 579Protein inscuteable homologAdd BLAST579

Proteomic databases

PaxDbiQ3HNM7.
PRIDEiQ3HNM7.

PTM databases

iPTMnetiQ3HNM7.
PhosphoSitePlusiQ3HNM7.

Expressioni

Tissue specificityi

Expressed in brain, kidney, liver, testis and skin.2 Publications

Developmental stagei

Expressed at E12.5 in dorsal root and cranial glanglia in the developing brain. Detected at E14.5 in skin epidermis (at protein level). First detected at E10.5 in the dorsal root glanglia and cranial glanglia. At E12.5 expression appears in retina, forebrain and outside of the nervous system. At E13.5 a strong expression is detected in the cortex. Expressed in embryonic, newborn and adult skin.1 Publication

Gene expression databases

BgeeiENSMUSG00000048782.
CleanExiMM_INSC.

Interactioni

Subunit structurei

Interacts with ALS2CR19/PAR3-beta and GPSM1/AGS3 (By similarity). Interacts with F2RL2/PAR3 (PubMed:16094321). Interacts with GPSM2/LGN (via TPR repeat region) (PubMed:16094321, PubMed:21816348).By similarity2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112682.

Structurei

Secondary structure

1579
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi73 – 82Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RO3X-ray1.10B66-87[»]
ProteinModelPortaliQ3HNM7.
SMRiQ3HNM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 89Important for interaction with GPSM21 PublicationAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi576 – 579PDZ-binding4

Phylogenomic databases

eggNOGiENOG410IHN9. Eukaryota.
ENOG410XSNH. LUCA.
HOGENOMiHOG000060207.
HOVERGENiHBG079606.
InParanoidiQ3HNM7.
PhylomeDBiQ3HNM7.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR031938. INSC_LBD.
[Graphical view]
PfamiPF16748. INSC_LBD. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3HNM7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRPPGDGDS TGEGPGNWGL WGAQESRRLC CVGPDRCGQA LLQIGINMMA
60 70 80 90 100
LPGGRHLDSV PLQEQRLHFM QVDSVQRWME DLKLMTECEC MCVLQAKPIS
110 120 130 140 150
LEEDTQGDLI LAGGPGPGDP LQLLLKRGWV ISTELRRIGQ KLAQDRWARV
160 170 180 190 200
HSMSVRLTCH ARSMVSEYST ISRTASQEMG QAEKLLMEKC SELSAVTERC
210 220 230 240 250
LQVENEHVLK SMKACVSETL SLLGEHFGQL LELALTREVQ ALVRKIDTSD
260 270 280 290 300
NIYITESTTG NLFGLTQEGA PLCRIIAKEG GVVALFKVCR QDSFRCLYPQ
310 320 330 340 350
ALRTLASICC VEEGVHQLEK VDGILCLADI LTDESHSEAT RAEAAAVVAQ
360 370 380 390 400
VTSPHLSFTQ HLTSFLENME EIVTALIKLC QEASSGEVFL LASAALANIT
410 420 430 440 450
FFDKMACEML LQLNAIRVLL EACGDKQRVD TPYTRDQIVT ILANMSVLEQ
460 470 480 490 500
CGSDIIQENG VQLIMGMLSE KPRSGTPAEV AACERVQQKA AVTLARLCRD
510 520 530 540 550
PDVAQEAVRL SCMSRLIELC RSPSERNSSD AVLVACLAAL RRLAGVCPEG
560 570
LQDSDFQQLV QPRLVDSFLL CSNMEESFV
Length:579
Mass (Da):63,615
Last modified:October 17, 2006 - v2
Checksum:iBE18903D7D66C830
GO
Isoform 2 (identifier: Q3HNM7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-186: Missing.

Show »
Length:393
Mass (Da):42,980
Checksum:iC9BAEBC62F1DCA77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti560V → L in ABA54267 (PubMed:16094321).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0209511 – 186Missing in isoform 2. 2 PublicationsAdd BLAST186

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050143 mRNA. Translation: BAC34091.1.
BY236076 mRNA. No translation available.
BC139196 mRNA. Translation: AAI39197.1.
BC139197 mRNA. Translation: AAI39198.1.
DQ205645 mRNA. Translation: ABA54267.1.
RefSeqiNP_776128.2. NM_173767.3.
XP_006507722.1. XM_006507659.3. [Q3HNM7-1]
UniGeneiMm.83037.

Genome annotation databases

GeneIDi233752.
KEGGimmu:233752.
UCSCiuc009jii.2. mouse. [Q3HNM7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050143 mRNA. Translation: BAC34091.1.
BY236076 mRNA. No translation available.
BC139196 mRNA. Translation: AAI39197.1.
BC139197 mRNA. Translation: AAI39198.1.
DQ205645 mRNA. Translation: ABA54267.1.
RefSeqiNP_776128.2. NM_173767.3.
XP_006507722.1. XM_006507659.3. [Q3HNM7-1]
UniGeneiMm.83037.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RO3X-ray1.10B66-87[»]
ProteinModelPortaliQ3HNM7.
SMRiQ3HNM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112682.

PTM databases

iPTMnetiQ3HNM7.
PhosphoSitePlusiQ3HNM7.

Proteomic databases

PaxDbiQ3HNM7.
PRIDEiQ3HNM7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi233752.
KEGGimmu:233752.
UCSCiuc009jii.2. mouse. [Q3HNM7-1]

Organism-specific databases

CTDi387755.
MGIiMGI:1917942. Insc.

Phylogenomic databases

eggNOGiENOG410IHN9. Eukaryota.
ENOG410XSNH. LUCA.
HOGENOMiHOG000060207.
HOVERGENiHBG079606.
InParanoidiQ3HNM7.
PhylomeDBiQ3HNM7.

Miscellaneous databases

ChiTaRSiInsc. mouse.
PROiQ3HNM7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048782.
CleanExiMM_INSC.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR031938. INSC_LBD.
[Graphical view]
PfamiPF16748. INSC_LBD. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiINSC_MOUSE
AccessioniPrimary (citable) accession number: Q3HNM7
Secondary accession number(s): B2RTA5, Q8C7I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.