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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with the host mitochondrial antiviral signaling protein MAVS.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2
Alternative name(s):
RNA-directed RNA polymerase subunit P3
Gene namesi
Name:PB2
OrganismiInfluenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
Taxonomic identifieri88776 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008430 Componenti: Genome

Subcellular locationi

  • Virion
  • Host nucleus By similarity
  • Host mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Polymerase basic protein 2PRO_0000310573Add
BLAST

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ3HM41.
SMRiQ3HM41. Positions 319-483, 685-759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi736 – 7394Nuclear localization signalBy similarity

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.Curated

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q3HM41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM
60 70 80 90 100
MAMKYPITAD KRIMEMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPTTSAVH YPKIYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV
260 270 280 290 300
RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVDILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG
460 470 480 490 500
IEPIDNVMGM IGILPDMTPS TEMSMRGVRV SKMGVDEYSS TERVVVSIDR
510 520 530 540 550
FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEVN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTVQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILVR
660 670 680 690 700
GNSPVFNYNK ATKRLTVLGK DAGALTEDPD EGTAGVESAV LRGFLILGKE
710 720 730 740 750
DRRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):85,938
Last modified:November 8, 2005 - v1
Checksum:iCCF3E21D026468F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ208309 mRNA. Translation: ABA55038.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ208309 mRNA. Translation: ABA55038.1.

3D structure databases

ProteinModelPortaliQ3HM41.
SMRiQ3HM41. Positions 319-483, 685-759.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the 1918 influenza virus polymerase genes."
    Taubenberger J.K., Reid A.H., Lourens R.M., Wang R., Jin G., Fanning T.G.
    Nature 437:889-893(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPB2_I18A0
AccessioniPrimary (citable) accession number: Q3HM41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 8, 2005
Last modified: June 24, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.