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Q3ECS3

- BGL35_ARATH

UniProt

Q3ECS3 - BGL35_ARATH

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Protein

Myrosinase 5

Gene

TGG5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.1 Publication

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=547 µM for sinigrin (at pH 4.5)1 Publication
  2. KM=80 mM for p-nitrophenyl beta-D-glucoside (at pH 4.5)1 Publication

Vmax=48.1 µmol/min/mg enzyme with sinigrin as substrate (at pH 4.5)1 Publication

Vmax=17 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate (at pH 4.5)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641SubstrateBy similarity
Binding sitei165 – 1651SubstrateBy similarity
Binding sitei210 – 2101SubstrateBy similarity
Binding sitei211 – 2111AscorbateBy similarity
Binding sitei280 – 2801AscorbateBy similarity
Binding sitei351 – 3511SubstrateBy similarity
Active sitei418 – 4181NucleophilePROSITE-ProRule annotation
Binding sitei467 – 4671SubstrateBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: TAIR
  2. thioglucosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ3ECS3.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 5 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 35 (EC:3.2.1.21)
Short name:
AtBGLU35
Sinigrinase 5
Thioglucosidase 5
Gene namesi
Name:TGG5
Synonyms:BGLU35
Ordered Locus Names:At1g51470
ORF Names:F5D21.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G51470.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 511488Myrosinase 5PRO_0000389597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 450By similarity
Disulfide bondi39 ↔ 445By similarity
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi230 ↔ 233By similarity
Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3ECS3.
PRIDEiQ3ECS3.

Expressioni

Tissue specificityi

Specifically expressed in roots.1 Publication

Gene expression databases

GenevestigatoriQ3ECS3.

Interactioni

Protein-protein interaction databases

BioGridi26797. 1 interaction.
STRINGi3702.AT1G51470.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ3ECS3.
SMRiQ3ECS3. Positions 46-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni474 – 4752Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ3ECS3.
KOiK01237.
OMAiDHILEME.
PhylomeDBiQ3ECS3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ECS3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIPKAHYSL AVLVLLFVVV SSSQKVCNPE CKAKEPFHCD NTHAFNRSGF
60 70 80 90 100
PKNFTFGAAT SAYQIEGAAH RALNGWDYFT HRYPEKVPDR SSADLACDSY
110 120 130 140 150
DLYKDDVKLL KRMNVQAYRL SIAWSRVLPK GRLTGGVDEN GITYYNNLIN
160 170 180 190 200
ELKANGIEPY VTIFHWDVPQ TLEDEYGGFL STRIVEDYTN YAELLFQRFG
210 220 230 240 250
DRVKFWITLN QPLSLALKGY GNGSYPPGRC TGCELGGDSG VEPYTVAHNQ
260 270 280 290 300
LLAHAKTVSL YRKRYQKFQG GKIGTTLIGR WFVPLNEFSE LDKAAAKRAF
310 320 330 340 350
DFFVGWFLDP LVYGKYPTIM REMVGDRLPE FTPEESALVK GSLDFLGLNY
360 370 380 390 400
YVSQYATDAP PPTQPNAITD ARVTLGFYRN GSPIGVVASS FVYYPPGFRQ
410 420 430 440 450
ILNYIKDNYK NPLTYITENG VADLDLGNVT LATALADNGR IQNHCSHLSC
460 470 480 490 500
LKCAMKDGCN VAGYFAWSLM DNYEFGNGYT LRFGMNWVNF TNPADRKEKA
510
SGKWFSKFLA K
Length:511
Mass (Da):57,468
Last modified:November 8, 2005 - v1
Checksum:i0CEA34FA0DFB95B5
GO

Sequence cautioni

The sequence AAG52628.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ268796 Genomic DNA. Translation: ACO95140.1.
AC024261 Genomic DNA. Translation: AAG52628.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32671.1.
PIRiA96553.
RefSeqiNP_175558.3. NM_104025.3.
UniGeneiAt.25235.
At.48300.

Genome annotation databases

EnsemblPlantsiAT1G51470.1; AT1G51470.1; AT1G51470.
GeneIDi841572.
KEGGiath:AT1G51470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ268796 Genomic DNA. Translation: ACO95140.1 .
AC024261 Genomic DNA. Translation: AAG52628.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE32671.1 .
PIRi A96553.
RefSeqi NP_175558.3. NM_104025.3.
UniGenei At.25235.
At.48300.

3D structure databases

ProteinModelPortali Q3ECS3.
SMRi Q3ECS3. Positions 46-511.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 26797. 1 interaction.
STRINGi 3702.AT1G51470.1-P.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi Q3ECS3.
PRIDEi Q3ECS3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G51470.1 ; AT1G51470.1 ; AT1G51470 .
GeneIDi 841572.
KEGGi ath:AT1G51470.

Organism-specific databases

TAIRi AT1G51470.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi Q3ECS3.
KOi K01237.
OMAi DHILEME.
PhylomeDBi Q3ECS3.

Enzyme and pathway databases

SABIO-RK Q3ECS3.

Gene expression databases

Genevestigatori Q3ECS3.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a new subfamily of thioglucoside glucohydrolases."
    Zhang J.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties."
    Andersson D., Chakrabarty R., Bejai S., Zhang J., Rask L., Meijer J.
    Phytochemistry 70:1345-1354(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

Entry informationi

Entry nameiBGL35_ARATH
AccessioniPrimary (citable) accession number: Q3ECS3
Secondary accession number(s): Q9C8J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 8, 2005
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3