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Protein

Myrosinase 5

Gene

TGG5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.1 Publication

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=547 µM for sinigrin (at pH 4.5)1 Publication
  2. KM=80 mM for p-nitrophenyl beta-D-glucoside (at pH 4.5)1 Publication
  1. Vmax=48.1 µmol/min/mg enzyme with sinigrin as substrate (at pH 4.5)1 Publication
  2. Vmax=17 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate (at pH 4.5)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateBy similarity1
Binding sitei165SubstrateBy similarity1
Binding sitei210SubstrateBy similarity1
Binding sitei211AscorbateBy similarity1
Binding sitei280AscorbateBy similarity1
Binding sitei351SubstrateBy similarity1
Active sitei418NucleophilePROSITE-ProRule annotation1
Binding sitei467SubstrateBy similarity1

GO - Molecular functioni

  • beta-glucosidase activity Source: TAIR
  • scopolin beta-glucosidase activity Source: UniProtKB-EC
  • thioglucosidase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ3ECS3.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 5 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 35 (EC:3.2.1.21)
Short name:
AtBGLU35
Sinigrinase 5
Thioglucosidase 5
Gene namesi
Name:TGG5
Synonyms:BGLU35
Ordered Locus Names:At1g51470
ORF Names:F5D21.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G51470.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000038959724 – 511Myrosinase 5Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 450By similarity
Disulfide bondi39 ↔ 445By similarity
Glycosylationi46N-linked (GlcNAc...)Sequence analysis1
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Glycosylationi222N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi230 ↔ 233By similarity
Glycosylationi428N-linked (GlcNAc...)Sequence analysis1
Glycosylationi489N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3ECS3.
PRIDEiQ3ECS3.

Expressioni

Tissue specificityi

Specifically expressed in roots.1 Publication

Gene expression databases

GenevisibleiQ3ECS3. AT.

Interactioni

Protein-protein interaction databases

BioGridi26797. 1 interactor.
STRINGi3702.AT1G51470.1.

Structurei

3D structure databases

ProteinModelPortaliQ3ECS3.
SMRiQ3ECS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni474 – 475Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ3ECS3.
KOiK01237.
OMAiCFEKRIL.
OrthoDBiEOG0936077Q.
PhylomeDBiQ3ECS3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ECS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPKAHYSL AVLVLLFVVV SSSQKVCNPE CKAKEPFHCD NTHAFNRSGF
60 70 80 90 100
PKNFTFGAAT SAYQIEGAAH RALNGWDYFT HRYPEKVPDR SSADLACDSY
110 120 130 140 150
DLYKDDVKLL KRMNVQAYRL SIAWSRVLPK GRLTGGVDEN GITYYNNLIN
160 170 180 190 200
ELKANGIEPY VTIFHWDVPQ TLEDEYGGFL STRIVEDYTN YAELLFQRFG
210 220 230 240 250
DRVKFWITLN QPLSLALKGY GNGSYPPGRC TGCELGGDSG VEPYTVAHNQ
260 270 280 290 300
LLAHAKTVSL YRKRYQKFQG GKIGTTLIGR WFVPLNEFSE LDKAAAKRAF
310 320 330 340 350
DFFVGWFLDP LVYGKYPTIM REMVGDRLPE FTPEESALVK GSLDFLGLNY
360 370 380 390 400
YVSQYATDAP PPTQPNAITD ARVTLGFYRN GSPIGVVASS FVYYPPGFRQ
410 420 430 440 450
ILNYIKDNYK NPLTYITENG VADLDLGNVT LATALADNGR IQNHCSHLSC
460 470 480 490 500
LKCAMKDGCN VAGYFAWSLM DNYEFGNGYT LRFGMNWVNF TNPADRKEKA
510
SGKWFSKFLA K
Length:511
Mass (Da):57,468
Last modified:November 8, 2005 - v1
Checksum:i0CEA34FA0DFB95B5
GO

Sequence cautioni

The sequence AAG52628 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ268796 Genomic DNA. Translation: ACO95140.1.
AC024261 Genomic DNA. Translation: AAG52628.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32671.1.
PIRiA96553.
RefSeqiNP_175558.3. NM_104025.4.
UniGeneiAt.25235.
At.48300.

Genome annotation databases

EnsemblPlantsiAT1G51470.1; AT1G51470.1; AT1G51470.
GeneIDi841572.
GrameneiAT1G51470.1; AT1G51470.1; AT1G51470.
KEGGiath:AT1G51470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ268796 Genomic DNA. Translation: ACO95140.1.
AC024261 Genomic DNA. Translation: AAG52628.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32671.1.
PIRiA96553.
RefSeqiNP_175558.3. NM_104025.4.
UniGeneiAt.25235.
At.48300.

3D structure databases

ProteinModelPortaliQ3ECS3.
SMRiQ3ECS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26797. 1 interactor.
STRINGi3702.AT1G51470.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ3ECS3.
PRIDEiQ3ECS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G51470.1; AT1G51470.1; AT1G51470.
GeneIDi841572.
GrameneiAT1G51470.1; AT1G51470.1; AT1G51470.
KEGGiath:AT1G51470.

Organism-specific databases

TAIRiAT1G51470.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ3ECS3.
KOiK01237.
OMAiCFEKRIL.
OrthoDBiEOG0936077Q.
PhylomeDBiQ3ECS3.

Enzyme and pathway databases

SABIO-RKQ3ECS3.

Miscellaneous databases

PROiQ3ECS3.

Gene expression databases

GenevisibleiQ3ECS3. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL35_ARATH
AccessioniPrimary (citable) accession number: Q3ECS3
Secondary accession number(s): Q9C8J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 8, 2005
Last modified: November 30, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.