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Protein

Myrosinase 5

Gene

TGG5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.1 Publication

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=547 µM for sinigrin (at pH 4.5)1 Publication
  2. KM=80 mM for p-nitrophenyl beta-D-glucoside (at pH 4.5)1 Publication
  1. Vmax=48.1 µmol/min/mg enzyme with sinigrin as substrate (at pH 4.5)1 Publication
  2. Vmax=17 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate (at pH 4.5)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641SubstrateBy similarity
Binding sitei165 – 1651SubstrateBy similarity
Binding sitei210 – 2101SubstrateBy similarity
Binding sitei211 – 2111AscorbateBy similarity
Binding sitei280 – 2801AscorbateBy similarity
Binding sitei351 – 3511SubstrateBy similarity
Active sitei418 – 4181NucleophilePROSITE-ProRule annotation
Binding sitei467 – 4671SubstrateBy similarity

GO - Molecular functioni

  • beta-glucosidase activity Source: TAIR
  • thioglucosidase activity Source: TAIR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ3ECS3.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 5 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 35 (EC:3.2.1.21)
Short name:
AtBGLU35
Sinigrinase 5
Thioglucosidase 5
Gene namesi
Name:TGG5
Synonyms:BGLU35
Ordered Locus Names:At1g51470
ORF Names:F5D21.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G51470.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 511488Myrosinase 5PRO_0000389597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 450By similarity
Disulfide bondi39 ↔ 445By similarity
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi230 ↔ 233By similarity
Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3ECS3.
PRIDEiQ3ECS3.

Expressioni

Tissue specificityi

Specifically expressed in roots.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi26797. 1 interaction.
STRINGi3702.AT1G51470.1.

Structurei

3D structure databases

ProteinModelPortaliQ3ECS3.
SMRiQ3ECS3. Positions 46-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni474 – 4752Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ3ECS3.
KOiK01237.
OMAiPNAITDA.
PhylomeDBiQ3ECS3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ECS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPKAHYSL AVLVLLFVVV SSSQKVCNPE CKAKEPFHCD NTHAFNRSGF
60 70 80 90 100
PKNFTFGAAT SAYQIEGAAH RALNGWDYFT HRYPEKVPDR SSADLACDSY
110 120 130 140 150
DLYKDDVKLL KRMNVQAYRL SIAWSRVLPK GRLTGGVDEN GITYYNNLIN
160 170 180 190 200
ELKANGIEPY VTIFHWDVPQ TLEDEYGGFL STRIVEDYTN YAELLFQRFG
210 220 230 240 250
DRVKFWITLN QPLSLALKGY GNGSYPPGRC TGCELGGDSG VEPYTVAHNQ
260 270 280 290 300
LLAHAKTVSL YRKRYQKFQG GKIGTTLIGR WFVPLNEFSE LDKAAAKRAF
310 320 330 340 350
DFFVGWFLDP LVYGKYPTIM REMVGDRLPE FTPEESALVK GSLDFLGLNY
360 370 380 390 400
YVSQYATDAP PPTQPNAITD ARVTLGFYRN GSPIGVVASS FVYYPPGFRQ
410 420 430 440 450
ILNYIKDNYK NPLTYITENG VADLDLGNVT LATALADNGR IQNHCSHLSC
460 470 480 490 500
LKCAMKDGCN VAGYFAWSLM DNYEFGNGYT LRFGMNWVNF TNPADRKEKA
510
SGKWFSKFLA K
Length:511
Mass (Da):57,468
Last modified:November 8, 2005 - v1
Checksum:i0CEA34FA0DFB95B5
GO

Sequence cautioni

The sequence AAG52628.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ268796 Genomic DNA. Translation: ACO95140.1.
AC024261 Genomic DNA. Translation: AAG52628.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32671.1.
PIRiA96553.
RefSeqiNP_175558.3. NM_104025.3.
UniGeneiAt.25235.
At.48300.

Genome annotation databases

EnsemblPlantsiAT1G51470.1; AT1G51470.1; AT1G51470.
GeneIDi841572.
KEGGiath:AT1G51470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ268796 Genomic DNA. Translation: ACO95140.1.
AC024261 Genomic DNA. Translation: AAG52628.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32671.1.
PIRiA96553.
RefSeqiNP_175558.3. NM_104025.3.
UniGeneiAt.25235.
At.48300.

3D structure databases

ProteinModelPortaliQ3ECS3.
SMRiQ3ECS3. Positions 46-511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26797. 1 interaction.
STRINGi3702.AT1G51470.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ3ECS3.
PRIDEiQ3ECS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G51470.1; AT1G51470.1; AT1G51470.
GeneIDi841572.
KEGGiath:AT1G51470.

Organism-specific databases

TAIRiAT1G51470.

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ3ECS3.
KOiK01237.
OMAiPNAITDA.
PhylomeDBiQ3ECS3.

Enzyme and pathway databases

SABIO-RKQ3ECS3.

Miscellaneous databases

PROiQ3ECS3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a new subfamily of thioglucoside glucohydrolases."
    Zhang J.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties."
    Andersson D., Chakrabarty R., Bejai S., Zhang J., Rask L., Meijer J.
    Phytochemistry 70:1345-1354(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

Entry informationi

Entry nameiBGL35_ARATH
AccessioniPrimary (citable) accession number: Q3ECS3
Secondary accession number(s): Q9C8J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 8, 2005
Last modified: July 22, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.