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Protein

General transcription and DNA repair factor IIH subunit TFB1-1

Gene

TFB1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.By similarity

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-ATH-113418 Formation of the Early Elongation Complex
R-ATH-5696395 Formation of Incision Complex in GG-NER
R-ATH-5696400 Dual Incision in GG-NER
R-ATH-674695 RNA Polymerase II Pre-transcription Events
R-ATH-6781823 Formation of TC-NER Pre-Incision Complex
R-ATH-6782135 Dual incision in TC-NER
R-ATH-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-ATH-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-ATH-72086 mRNA Capping
R-ATH-73776 RNA Polymerase II Promoter Escape
R-ATH-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-ATH-75953 RNA Polymerase II Transcription Initiation
R-ATH-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-ATH-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription and DNA repair factor IIH subunit TFB1-1
Short name:
AtTFB1-11 Publication
Short name:
TFIIH subunit TFB1-1
Alternative name(s):
RNA polymerase II transcription factor B subunit 1-1
Gene namesi
Name:TFB1-1Curated
Synonyms:GTF2H1-1Curated
Ordered Locus Names:At1g55750
ORF Names:F20N2.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G55750
TAIRilocus:2020447 AT1G55750

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004060921 – 591General transcription and DNA repair factor IIH subunit TFB1-1Add BLAST591

Proteomic databases

PaxDbiQ3ECP0

Expressioni

Gene expression databases

ExpressionAtlasiQ3ECP0 baseline
GenevisibleiQ3ECP0 AT

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB, XPD, TFB1/GTF2H1, GTF2H2/P44, TFB4/GTF2H3, TFB2/GTF2H4 and TFB5/GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CYCH1/cyclin H1, CDKD and MAT1/At4g30820 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.By similarity1 Publication

Protein-protein interaction databases

BioGridi272493 interactors.
IntActiQ3ECP0 7 interactors.
STRINGi3702.AT1G55750.1

Structurei

3D structure databases

ProteinModelPortaliQ3ECP0
SMRiQ3ECP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini112 – 166BSD 1PROSITE-ProRule annotationAdd BLAST55
Domaini191 – 243BSD 2PROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Belongs to the TFB1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2074 Eukaryota
ENOG410XRI6 LUCA
HOGENOMiHOG000006252
InParanoidiQ3ECP0
KOiK03141
OMAiEQNGEPS
OrthoDBiEOG093608QO
PhylomeDBiQ3ECP0

Family and domain databases

InterProiView protein in InterPro
IPR005607 BSD_dom
IPR027079 Tfb1/GTF2H1
IPR013876 TFIIH_BTF_p62_N
PANTHERiPTHR12856 PTHR12856, 1 hit
PfamiView protein in Pfam
PF03909 BSD, 1 hit
PF08567 PH_TFIIH, 1 hit
SMARTiView protein in SMART
SM00751 BSD, 2 hits
PROSITEiView protein in PROSITE
PS50858 BSD, 2 hits

Sequencei

Sequence statusi: Complete.

Q3ECP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGQIEKLV KYKSTVKDPG TPGFLRIREG MLLFVPNDPK SDSKLKVLTQ
60 70 80 90 100
NIKSQKYTKE GSNKPPWLNL TNKQAKSHIF EFENYPDMHA CRDFITKALA
110 120 130 140 150
KCELEPNKSV VSTSSEQLSI KELELRFKLL RENSELQRLH KQFVESKVLT
160 170 180 190 200
EDEFWATRKK LLGKDSIRKS KQQLGLKSMM VSGIKPSTDG RTNRVTFNLT
210 220 230 240 250
PEIIFQIFAE KPAVRQAFIN YVPSKMTEKD FWTKYFRAEY LYSTKNTAVA
260 270 280 290 300
AAEAAEDEEL AVFLKPDEIL ARETRHKIRR VDPTLDMEAD QGDDYTHLMD
310 320 330 340 350
HGIQRDGTMD VVEPQNDQFK RSLLQDLNRH AAVVLEGRSI DVESEDTRIV
360 370 380 390 400
AEALTRVKQV SKADGETTKD ANQERLERMS RVAGMEDLQA PQNFPLAPLS
410 420 430 440 450
IKDPRDYFES QQGNVLNVPR GAKGLKRNVH EAYGLLKESI LEIRATGLSD
460 470 480 490 500
PLIKPEVSFE VFSSLTRTIA TAKNINGKNP RESFLDRLPK STKDEVLHHW
510 520 530 540 550
TSIQELLKHF WSSYPITTTY LHTKVGKLKD AMSNTYSKLE AMKESVQSDL
560 570 580 590
RHQVSLLVRP MQQALDAAFH HYEVDLQRRT AKSGERPNGY V
Length:591
Mass (Da):67,514
Last modified:November 8, 2005 - v1
Checksum:i26CEAA2CE8178C2A
GO

Sequence cautioni

The sequence AAF79503 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g55750 and At1g55760.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002328 Genomic DNA Translation: AAF79503.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33293.1
CP002684 Genomic DNA Translation: ANM59713.1
AK226523 mRNA Translation: BAE98663.1
RefSeqiNP_001319242.1, NM_001333725.1
NP_175971.3, NM_104451.4
UniGeneiAt.42907

Genome annotation databases

EnsemblPlantsiAT1G55750.1; AT1G55750.1; AT1G55750
AT1G55750.6; AT1G55750.6; AT1G55750
GeneIDi842024
GrameneiAT1G55750.1; AT1G55750.1; AT1G55750
AT1G55750.6; AT1G55750.6; AT1G55750
KEGGiath:AT1G55750

Similar proteinsi

Entry informationi

Entry nameiTFB1A_ARATH
AccessioniPrimary (citable) accession number: Q3ECP0
Secondary accession number(s): Q9LFZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 8, 2005
Last modified: April 25, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome