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Protein

RNA pseudouridine synthase 2, chloroplastic

Gene

At1g76050

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

RNA uridine = RNA pseudouridine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei234By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciARA:AT1G76050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA pseudouridine synthase 2, chloroplastic (EC:5.4.99.-)
Alternative name(s):
RNA pseudouridylate synthase 2
RNA-uridine isomerase 2
Gene namesi
Ordered Locus Names:At1g76050
ORF Names:T4O12.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G76050.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43ChloroplastSequence analysisAdd BLAST43
ChainiPRO_000036332844 – 430RNA pseudouridine synthase 2, chloroplasticAdd BLAST387

Proteomic databases

PaxDbiQ3ECD0.

Expressioni

Gene expression databases

GenevisibleiQ3ECD0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G76050.2.

Structurei

3D structure databases

ProteinModelPortaliQ3ECD0.
SMRiQ3ECD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 155S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Belongs to the pseudouridine synthase RluA family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1919. Eukaryota.
COG0564. LUCA.
HOGENOMiHOG000275919.
InParanoidiQ3ECD0.
KOiK06180.
OMAiTSQNGMD.
OrthoDBiEOG09360AIC.
PhylomeDBiQ3ECD0.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006225. PsdUridine_synth_RluC/D.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00005. rluA_subfam. 1 hit.
PROSITEiPS01129. PSI_RLU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3ECD0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSISQLPSF SLTTAKSLRY PSSPSSSLSI FFSFFPKVSN FVRASSGIPN
60 70 80 90 100
LVACSPTEII IPRVNNAGLR IEEIVDAAKG KIRLDSWISS RINGVSRARV
110 120 130 140 150
QSSIRLGLVT VNGRVVDKVS HNVKSGDEVN CTISELQPLK AEAEDIPLDI
160 170 180 190 200
VYEDKHVLVV NKPAHMVVHP APGNPTGTLV NGILHHCSLP CVDYSNSEED
210 220 230 240 250
DDSDEETFSD DEEMTTSPSS YAASVRPGIV HRLDKGTTGL LVVAKDEHSH
260 270 280 290 300
AHLAEQFKLH TIERVYVSLT TGVPSPPQGR IEIPIGRDSS NRIRMAAIPG
310 320 330 340 350
GVRGGRARHA ASRYKVIETF AGGGSALVEW RLETGRTHQI RAHAKYMGVP
360 370 380 390 400
LLGDEVYGGT KSMALSLLQK RVSRSDQEEI IELISRMDRP CLHAIVLGFT
410 420 430
HPCTGEIVKF SCPPPSDLAE IVGLLRRSGL
Length:430
Mass (Da):46,715
Last modified:November 8, 2005 - v1
Checksum:i9EF980B7710967E3
GO
Isoform 2 (identifier: Q3ECD0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-430: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:313
Mass (Da):33,855
Checksum:i1801613C7A571EF7
GO

Sequence cautioni

The sequence AAF26765 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g76040 and At1g76050.Curated
The sequence BAC42428 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BX816325 differs from that shown. Sequencing errors.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036289314 – 430Missing in isoform 2. 1 PublicationAdd BLAST117

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007396 Genomic DNA. Translation: AAF26765.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35789.1.
CP002684 Genomic DNA. Translation: AEE35790.1.
BX816325 mRNA. No translation available.
AK117781 mRNA. Translation: BAC42428.1. Different initiation.
RefSeqiNP_177732.2. NM_106254.3. [Q3ECD0-2]
NP_974151.1. NM_202422.3. [Q3ECD0-1]
UniGeneiAt.34707.
At.68569.
At.71876.

Genome annotation databases

EnsemblPlantsiAT1G76050.2; AT1G76050.2; AT1G76050. [Q3ECD0-1]
GeneIDi843937.
GrameneiAT1G76050.2; AT1G76050.2; AT1G76050.
KEGGiath:AT1G76050.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007396 Genomic DNA. Translation: AAF26765.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35789.1.
CP002684 Genomic DNA. Translation: AEE35790.1.
BX816325 mRNA. No translation available.
AK117781 mRNA. Translation: BAC42428.1. Different initiation.
RefSeqiNP_177732.2. NM_106254.3. [Q3ECD0-2]
NP_974151.1. NM_202422.3. [Q3ECD0-1]
UniGeneiAt.34707.
At.68569.
At.71876.

3D structure databases

ProteinModelPortaliQ3ECD0.
SMRiQ3ECD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G76050.2.

Proteomic databases

PaxDbiQ3ECD0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G76050.2; AT1G76050.2; AT1G76050. [Q3ECD0-1]
GeneIDi843937.
GrameneiAT1G76050.2; AT1G76050.2; AT1G76050.
KEGGiath:AT1G76050.

Organism-specific databases

TAIRiAT1G76050.

Phylogenomic databases

eggNOGiKOG1919. Eukaryota.
COG0564. LUCA.
HOGENOMiHOG000275919.
InParanoidiQ3ECD0.
KOiK06180.
OMAiTSQNGMD.
OrthoDBiEOG09360AIC.
PhylomeDBiQ3ECD0.

Enzyme and pathway databases

BioCyciARA:AT1G76050-MONOMER.

Miscellaneous databases

PROiQ3ECD0.

Gene expression databases

GenevisibleiQ3ECD0. AT.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006225. PsdUridine_synth_RluC/D.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00005. rluA_subfam. 1 hit.
PROSITEiPS01129. PSI_RLU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUS2_ARATH
AccessioniPrimary (citable) accession number: Q3ECD0
Secondary accession number(s): Q8GY94, Q9LQR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 8, 2005
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.