Q3EC77 (PMT5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable methyltransferase PMT5
EC=2.1.1.-

Gene names

Ordered Locus Names:	At2g03480
ORF Names:	T4M8.9

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the methyltransferase superfamily.
Sequence caution	The sequence AAD17428.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAK63953.1 differs from that shown. Reason: Intron retention. The sequence AAN46794.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3EC77-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3EC77-2)

The sequence of this isoform differs from the canonical sequence as follows:
     402-426: KRWISIQNRSAVAGTTSAGLEIHGK → LKPEEFFEDTQIWR

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	Probable methyltransferase PMT5		PRO_0000393245
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	42 – 606	565	Lumenal Potential
	Compositional bias	46 – 50	5	Poly-Ser
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	402 – 426	25	KRWIS…EIHGK → LKPEEFFEDTQIWR in isoform 2.		VSP_038902

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 23, 2010. Version 2. Checksum: B2797F86E6B05EBB	FASTA	606	67,819
	10 20 30 40 50 60 MRGSWYKSVS SVFGLRPRIR GLLFFIVGVV ALVTILAPLT SNSYDSSSSS TLVPNIYSNY 70 80 90 100 110 120 RRIKEQAAVD YLDLRSLSLG ASLKEFPFCG KERESYVPCY NITGNLLAGL QEGEELDRHC 130 140 150 160 170 180 EFEREKERCV VRPPRDYKIP LRWPLGRDII WSGNVKITKD QFLSSGTVTT RLMLLEENQI 190 200 210 220 230 240 TFHSEDGLVF DGVKDYARQI AEMIGLGSDT EFAQAGVRTV LDIGCGFGSF GAHLVSLKLM 250 260 270 280 290 300 PICIAEYEAT GSQVQLALER GLPAMIGNFF SKQLPYPALS FDMVHCAQCG TTWDIKDAML 310 320 330 340 350 360 LLEVDRVLKP GGYFVLTSPT NKAQGNLPDT KKTSISTRVN ELSKKICWSL TAQQDETFLW 370 380 390 400 410 420 QKTSDSSCYS SRSQASIPLC KDGDSVPYYH PLVPCISGTT SKRWISIQNR SAVAGTTSAG 430 440 450 460 470 480 LEIHGKSALK NYWSLLTPLI FSDHPKRPGD EDPLPPFNMI RNVMDMHARF GNLNAALLDE 490 500 510 520 530 540 GKSAWVMNVV PVNARNTLPI ILDRGFAGVL HDWCEPFPTY PRTYDMLHAN ELLTHLSSER 550 560 570 580 590 600 CSLMDLFLEM DRILRPEGWV VLSDKVGVIE MARALAARVR WEARVIDLQD GSDQRLLVCQ KPFIKK
	Isoform 2 [UniParc]. Checksum: C5F92C3A3C268300	FASTA	595	66,976
	10 20 30 40 50 60 MRGSWYKSVS SVFGLRPRIR GLLFFIVGVV ALVTILAPLT SNSYDSSSSS TLVPNIYSNY 70 80 90 100 110 120 RRIKEQAAVD YLDLRSLSLG ASLKEFPFCG KERESYVPCY NITGNLLAGL QEGEELDRHC 130 140 150 160 170 180 EFEREKERCV VRPPRDYKIP LRWPLGRDII WSGNVKITKD QFLSSGTVTT RLMLLEENQI 190 200 210 220 230 240 TFHSEDGLVF DGVKDYARQI AEMIGLGSDT EFAQAGVRTV LDIGCGFGSF GAHLVSLKLM 250 260 270 280 290 300 PICIAEYEAT GSQVQLALER GLPAMIGNFF SKQLPYPALS FDMVHCAQCG TTWDIKDAML 310 320 330 340 350 360 LLEVDRVLKP GGYFVLTSPT NKAQGNLPDT KKTSISTRVN ELSKKICWSL TAQQDETFLW 370 380 390 400 410 420 QKTSDSSCYS SRSQASIPLC KDGDSVPYYH PLVPCISGTT SLKPEEFFED TQIWRSALKN 430 440 450 460 470 480 YWSLLTPLIF SDHPKRPGDE DPLPPFNMIR NVMDMHARFG NLNAALLDEG KSAWVMNVVP 490 500 510 520 530 540 VNARNTLPII LDRGFAGVLH DWCEPFPTYP RTYDMLHANE LLTHLSSERC SLMDLFLEMD 550 560 570 580 590 RILRPEGWVV LSDKVGVIEM ARALAARVRW EARVIDLQDG SDQRLLVCQK PFIKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative methyltransferase is required for cell adhesion and co-ordinated plant development." Krupkova E., Immerzeel P., Pauly M., Schmulling T. Plant J. 50:735-750(2007) [PubMed: 17461780] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006284 Genomic DNA. Translation: AAD17428.2. Sequence problems. CP002685 Genomic DNA. Translation: AEC05705.1. CP002685 Genomic DNA. Translation: AEC05706.1. AY039849 mRNA. Translation: AAK63953.1. Sequence problems. BT001040 mRNA. Translation: AAN46794.1. Sequence problems.
IPI	IPI00518265. IPI00535373.
PIR	A84449.
RefSeq	NP_027543.2. NM_126399.2. NP_973410.1. NM_201681.2.
UniGene	At.24474.
3D structure databases
ProteinModelPortal	Q3EC77.
SMR	Q3EC77. Positions 193-321.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3EC77.
Proteomic databases
PRIDE	Q3EC77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G03480.1; AT2G03480.1; AT2G03480.
GeneID	814877.
GenomeReviews	Gene locus AT2G03480 in contig CT485783_GR.
KEGG	ath:AT2G03480.
NMPDR	fig|3702.1.peg.7941.
Organism-specific databases
TAIR	At2g03480.
Phylogenomic databases
GeneTree	EPGT00070000028281.
HOGENOM	HBG597309.
InParanoid	Q3EC77.
OMA	IWSGNVK.
PhylomeDB	Q3EC77.
ProtClustDB	CLSN2682730.
Gene expression databases
Genevestigator	Q3EC77.
Family and domain databases
InterPro	IPR004159. DUF248_MeTrfase. [Graphical view]
Pfam	PF03141. DUF248. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMT5_ARATH

Accession

Primary (citable) accession number: Q3EC77
Secondary accession number(s): Q8H0Z7, Q94BV9, Q9ZQ84

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	March 23, 2010
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program