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Protein

Probable protein S-acyltransferase 23

Gene

PAT23

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Palmitoyl acyltransferase.By similarity1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei393S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein S-acyltransferase 23 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At2g14255
Zinc finger DHHC domain-containing protein At2g14255
Gene namesi
Name:PAT23
Ordered Locus Names:At2g14255
ORF Names:T1O16.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRilocus:1006230134. AT2G14255.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei270 – 290HelicalSequence analysisAdd BLAST21
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003635901 – 536Probable protein S-acyltransferase 23Add BLAST536

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ3EC11.

Expressioni

Tissue specificityi

Expressed in roots, shoots, flowers and pollen.1 Publication

Gene expression databases

GenevisibleiQ3EC11. AT.

Interactioni

Protein-protein interaction databases

BioGridi30135. 1 interactor.
STRINGi3702.AT2G14255.1.

Structurei

3D structure databases

ProteinModelPortaliQ3EC11.
SMRiQ3EC11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 86ANK 1Add BLAST30
Repeati90 – 119ANK 2Add BLAST30
Repeati123 – 153ANK 3Add BLAST31
Repeati157 – 186ANK 4Add BLAST30
Repeati190 – 219ANK 5Add BLAST30
Repeati225 – 254ANK 6Add BLAST30
Domaini363 – 413DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0509. Eukaryota.
COG0666. LUCA.
COG5273. LUCA.
HOGENOMiHOG000243854.
InParanoidiQ3EC11.
KOiK20032.
OMAiDIDIDHR.
OrthoDBiEOG093605DC.
PhylomeDBiQ3EC11.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF12796. Ank_2. 2 hits.
PF01529. zf-DHHC. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 6 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50216. DHHC. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q3EC11-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSSEIEVVP LDSNSHQSPT ESPTITDVFS ASAYGDLHQL KHFVEHNGSS
60 70 80 90 100
VSLPDDNGFY ALQWAALNNS LHVAQYIIQH GGDVNSADNI QQTPLHWAAV
110 120 130 140 150
KGSIDVADLL LQHGARIEAV DVNGFRAVHV ASQYGQTAFV NHIIVDYAAD
160 170 180 190 200
YNALDIEGRS PLHWAAYNGF TETVRLLLFR DACQNRQDNT GCTPLHWAVI
210 220 230 240 250
KENVEACTLL VHAGTKEELI LKDNTGSTPL KLASDKGHRQ LALFLSKAMR
260 270 280 290 300
TRKNSFVDKI FCGKLGETSY APMLFSLIVI LMVLFITSIV SASNLPKITA
310 320 330 340 350
MVGLWACFGL SCGVYALITF YRVSRKDPGY VKRTGEANSQ HTANDPLIDI
360 370 380 390 400
NFKNPSWKGN WSQLCPTCKI IRPVRSKHCP TCKRCVEQFD HHCPWISNCV
410 420 430 440 450
GKKNKRYFLV FVIMGALTSF VGGTTAVQRL WRGIPQVHHG ESWIKHIVIE
460 470 480 490 500
HPDAAVFLFF DLLIFIATMT LTISQSYMIA RNITTNELWN AKRFSYLRGP
510 520 530
DGRFYNPYNH GLRRNCTDFL VHGYTRDDEV VPSSIL
Length:536
Mass (Da):59,988
Last modified:July 1, 2008 - v2
Checksum:i1BBB18C651003487
GO

Sequence cautioni

The sequence AAD20110 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246 – 249SKAM → VRNV in ABN04785 (Ref. 3) Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006304 Genomic DNA. Translation: AAD20110.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06292.1.
BT030047 mRNA. Translation: ABN04785.1.
PIRiD84515.
RefSeqiNP_973453.2. NM_201724.3. [Q3EC11-1]
UniGeneiAt.40568.

Genome annotation databases

EnsemblPlantsiAT2G14255.1; AT2G14255.1; AT2G14255. [Q3EC11-1]
GeneIDi2745525.
GrameneiAT2G14255.1; AT2G14255.1; AT2G14255.
KEGGiath:AT2G14255.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006304 Genomic DNA. Translation: AAD20110.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06292.1.
BT030047 mRNA. Translation: ABN04785.1.
PIRiD84515.
RefSeqiNP_973453.2. NM_201724.3. [Q3EC11-1]
UniGeneiAt.40568.

3D structure databases

ProteinModelPortaliQ3EC11.
SMRiQ3EC11.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30135. 1 interactor.
STRINGi3702.AT2G14255.1.

Proteomic databases

PaxDbiQ3EC11.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G14255.1; AT2G14255.1; AT2G14255. [Q3EC11-1]
GeneIDi2745525.
GrameneiAT2G14255.1; AT2G14255.1; AT2G14255.
KEGGiath:AT2G14255.

Organism-specific databases

AraportiAT2G14255.
TAIRilocus:1006230134. AT2G14255.

Phylogenomic databases

eggNOGiKOG0509. Eukaryota.
COG0666. LUCA.
COG5273. LUCA.
HOGENOMiHOG000243854.
InParanoidiQ3EC11.
KOiK20032.
OMAiDIDIDHR.
OrthoDBiEOG093605DC.
PhylomeDBiQ3EC11.

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Miscellaneous databases

PROiPR:Q3EC11.

Gene expression databases

GenevisibleiQ3EC11. AT.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF12796. Ank_2. 2 hits.
PF01529. zf-DHHC. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 6 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50216. DHHC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZDHC2_ARATH
AccessioniPrimary (citable) accession number: Q3EC11
Secondary accession number(s): A2RVQ6, Q9ZQ58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: July 1, 2008
Last modified: April 12, 2017
This is version 88 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.