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Protein

Probable protein S-acyltransferase 17

Gene

PAT17

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Palmitoyl acyltransferase.By similarity1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein S-acyltransferase 17 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At3g04970
Zinc finger DHHC domain-containing protein At3g04970
Gene namesi
Name:PAT17
Ordered Locus Names:At3g04970
ORF Names:T9J14.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G04970.
TAIRilocus:2114895. AT3G04970.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2 – 22HelicalSequence analysisAdd BLAST21
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Transmembranei83 – 103HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003635931 – 397Probable protein S-acyltransferase 17Add BLAST397

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ3EBC2.

Expressioni

Gene expression databases

ExpressionAtlasiQ3EBC2. baseline and differential.
GenevisibleiQ3EBC2. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G04970.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 210DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1312. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000006420.
InParanoidiQ3EBC2.
KOiK20003.
OMAiMAVQWLL.
OrthoDBiEOG09360FTO.
PhylomeDBiQ3EBC2.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3EBC2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVQWLLVCH GMMTLTVVIS FLCGQWPIFK GTPFQWIHYF LTFGAYDYFL
60 70 80 90 100
RFVGFVFGSK GTDVILSVEY FCCDRPNPIL QVIYIAIMGS TYFLTAKSSF
110 120 130 140 150
IYIPGYYLGD VHKYTSFLAV IVGVILFLLT CFSDPGTVNA ENVSRYISAY
160 170 180 190 200
PYDDIIYSKK ECSTCKIPKP ARSKHCSICN RCVARFDHHC GWMNNCIGER
210 220 230 240 250
NTKYFMAFLL WHFLLCLYGT VAIGFILAGR VKELRVVHIL TVYYGVDKSF
260 270 280 290 300
RSLAPRVIQW LVGTYNTQIL LMVFLAIVSL LLAGFFAYHA NLCLTNTTTN
310 320 330 340 350
ETFKWREYIS LNKKLSEAKA SAAALKAGMS CELKKPSAES KCFGLCGRSS
360 370 380 390
AREEEVKADA IAKRNLYDRG SFQNVSEIVF PLSSRPSSSN KSKRKSE
Length:397
Mass (Da):44,946
Last modified:November 8, 2005 - v1
Checksum:i1B179FB4FE9E7CF7
GO
Isoform 2 (identifier: Q3EBC2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     302-316: TFKWREYISLNKKLS → VLTFPSLEINYVSPS
     317-397: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:316
Mass (Da):36,003
Checksum:iA590B76174A2F520
GO

Sequence cautioni

The sequence AAG51421 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74D → N in BX824996 (PubMed:14993207).Curated1
Sequence conflicti151P → T in BAF00409 (Ref. 4) Curated1
Sequence conflicti153D → N in BX824996 (PubMed:14993207).Curated1
Sequence conflicti183V → M in BX824996 (PubMed:14993207).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036314302 – 316TFKWR…NKKLS → VLTFPSLEINYVSPS in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_036315317 – 397Missing in isoform 2. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009465 Genomic DNA. Translation: AAG51421.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74169.1.
CP002686 Genomic DNA. Translation: AEE74170.1.
BX824996 mRNA. No translation available.
AK228483 mRNA. Translation: BAF00409.1.
RefSeqiNP_187148.2. NM_111369.4. [Q3EBC2-1]
NP_974224.1. NM_202495.2. [Q3EBC2-2]
UniGeneiAt.44032.

Genome annotation databases

EnsemblPlantsiAT3G04970.1; AT3G04970.1; AT3G04970. [Q3EBC2-1]
GeneIDi819657.
GrameneiAT3G04970.1; AT3G04970.1; AT3G04970. [Q3EBC2-1]
KEGGiath:AT3G04970.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiZDHC5_ARATH
AccessioniPrimary (citable) accession number: Q3EBC2
Secondary accession number(s): Q0WR40, Q9CAW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 8, 2005
Last modified: July 5, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families