Reviewed, UniProtKB/Swiss-Prot Q3EAY9 (PME30_ARATH)

Names and origin

Protein names

Recommended name:
    Probable pectinesterase 30
      Short name=PE 30
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 30
      Short name=AtPME30

Gene names

Name:	PME30
Synonyms:	ARATH30
Ordered Locus Names:	At3g27980
ORF Names:	K24A2.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in siliques. Ref.4
Developmental stage	Expressed during late developmental phases of siliques. Ref.4
Sequence similarities	Belongs to the pectinesterase family.
Sequence caution	The sequence ABK28786.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay. Source: TAIR
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 497	476	Probable pectinesterase 30		PRO_0000370186
Sites
	Active site	316	1	Proton donor By similarity
	Active site	337	1	Nucleophile By similarity
	Binding site	263	1	Substrate By similarity
	Binding site	403	1	Substrate By similarity
	Binding site	405	1	Substrate By similarity
	Site	315	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	385	1	N-linked (GlcNAc...) Potential
	Disulfide bond	330 ↔ 350		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3EAY9-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: ACBB9C1E6E881284	FASTA	497	55,459
	10 20 30 40 50 60 MLVKVFSFFI LMIIMVIGVS KEYCDDKQSC QNLLLELKAG SSSLSEIRRR DLLIIVLKNS 70 80 90 100 110 120 VRRIDMAMIG VMDDTKQHEE MENDMLGVKE DTNLFEEMME SEENSHTWLS SVLTSYITCI 130 140 150 160 170 180 DEIGEGAYKR RVEPKLENLI SRARVVLALF ISISLRDNTE LISVIPNGPS WLFHVDKKDL 190 200 210 220 230 240 YLNAEIADVV VAKDGTGKYS TVNAAIAAAP QHSQKRFVIY IKTGIYDEIV VIENTKPNLT 250 260 270 280 290 300 LIGDGQDLTI ITGNLSASNV RRTYNTATVA SNGNGFIGVD MCFRNTAGPA KGPAVALRVS 310 320 330 340 350 360 GDMSVIYRCR VEGYQDALYP HSDRQFYREC FITGTVDFIC GNAVAVFQFC QIVARQPKMG 370 380 390 400 410 420 QSNVITAQSR ATKDVKSGFS IQNCNITTSS DLDTATVKTY LGRPWRRFST VAVLQSFIGD 430 440 450 460 470 480 LVDPAGWTPW KGETGLSTLH YREYQNRGPG AVTSRRVKWS GFKVMKDPKK ATEFTVAKLL 490 DGETWLKESR IPYESGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[4]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP001302 Genomic DNA. No translation available. DQ653407 mRNA. Translation: ABK28786.1. Sequence problems.
IPI	IPI00527306.
RefSeq	NP_189437.1.
UniGene	At.51376
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	822422.
GenomeReviews	Gene locus AT3G27980 in contig BA000014_GR.
KEGG	ath:AT3G27980.
NMPDR	fig|3702.1.peg.15089.
Organism-specific databases
TAIR	At3g27980.
Gene expression databases
Genevestigator	Q3EAY9.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. 1 hit. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME30_ARATH

Accession

Primary (citable) accession number: Q3EAY9
Secondary accession number(s): A0MFT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 8, 2005
Last modified:	October 13, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)