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Q3EAF9

- P2C49_ARATH

UniProt

Q3EAF9 - P2C49_ARATH

Protein

Probable protein phosphatase 2C 49

Gene

At3g62260

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi122 – 1221Manganese 1By similarity
    Metal bindingi122 – 1221Manganese 2By similarity
    Metal bindingi123 – 1231Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi297 – 2971Manganese 2By similarity
    Metal bindingi340 – 3401Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. protein dephosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G62260-MONOMER.
    ARA:GQT-2642-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable protein phosphatase 2C 49 (EC:3.1.3.16)
    Short name:
    AtPP2C49
    Gene namesi
    Ordered Locus Names:At3g62260
    ORF Names:T17J13.220
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G62260.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 384384Probable protein phosphatase 2C 49PRO_0000367973Add
    BLAST

    Proteomic databases

    PRIDEiQ3EAF9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ3EAF9.

    Interactioni

    Protein-protein interaction databases

    BioGridi10713. 3 interactions.
    IntActiQ3EAF9. 1 interaction.
    STRINGi3702.AT3G62260.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3EAF9.
    SMRiQ3EAF9. Positions 77-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 342266PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000233896.
    InParanoidiQ3EAF9.
    KOiK14803.
    OMAiVMTSQHA.
    PhylomeDBiQ3EAF9.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3EAF9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVAEAEVVFQ QSLPAVLEIE LFDGVSSAVK SPVSSPKLGF TQSTASVSGS    50
    LTTSPVQADI FPEGDCDPSV LDYIPTIRSG SFADIGPKRN MEDEHIRIDD 100
    LSSQVGSLFE LPKPSAFYAV FDGHGGPEAA AYVRENAIRF FFEDEQFPQT 150
    SEVSSVYVEE VETSLRNAFL QADLALAEDC SISDSCGTTA LTALICGRLL 200
    MVANAGDCRA VLCRKGRAID MSEDHKPINL LERRRVEESG GFITNDGYLN 250
    EVLAVTRALG DWDLKLPHGS QSPLISEPEI KQITLTEDDE FLVIGCDGIW 300
    DVLTSQEAVS IVRRGLNRHN DPTRCARELV MEALGRNSFD NLTAVVVCFM 350
    TMDRGDKPVV PLEKRRCFSL SPEAFCSLRN LLDG 384

    Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

    Length:384
    Mass (Da):41,968
    Last modified:November 8, 2005 - v1
    Checksum:i70C776A812AED76A
    GO
    Isoform 2 (identifier: Q3EAF9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         57-57: Missing.

    Show »
    Length:383
    Mass (Da):41,840
    Checksum:i3ED36648FF529C0A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei57 – 571Missing in isoform 2. 3 PublicationsVSP_036774

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138651 Genomic DNA. Translation: CAB71886.1.
    CP002686 Genomic DNA. Translation: AEE80328.1.
    CP002686 Genomic DNA. Translation: AEE80329.1.
    AK316866 mRNA. Translation: BAH19574.1.
    BT030025 mRNA. Translation: ABN04763.1.
    AY085949 mRNA. Translation: AAM63159.1.
    PIRiT48018.
    RefSeqiNP_191785.1. NM_116091.3. [Q3EAF9-2]
    NP_850737.1. NM_180406.1. [Q3EAF9-1]
    UniGeneiAt.43534.

    Genome annotation databases

    EnsemblPlantsiAT3G62260.2; AT3G62260.2; AT3G62260. [Q3EAF9-1]
    GeneIDi825399.
    KEGGiath:AT3G62260.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138651 Genomic DNA. Translation: CAB71886.1 .
    CP002686 Genomic DNA. Translation: AEE80328.1 .
    CP002686 Genomic DNA. Translation: AEE80329.1 .
    AK316866 mRNA. Translation: BAH19574.1 .
    BT030025 mRNA. Translation: ABN04763.1 .
    AY085949 mRNA. Translation: AAM63159.1 .
    PIRi T48018.
    RefSeqi NP_191785.1. NM_116091.3. [Q3EAF9-2 ]
    NP_850737.1. NM_180406.1. [Q3EAF9-1 ]
    UniGenei At.43534.

    3D structure databases

    ProteinModelPortali Q3EAF9.
    SMRi Q3EAF9. Positions 77-349.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 10713. 3 interactions.
    IntActi Q3EAF9. 1 interaction.
    STRINGi 3702.AT3G62260.2-P.

    Proteomic databases

    PRIDEi Q3EAF9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G62260.2 ; AT3G62260.2 ; AT3G62260 . [Q3EAF9-1 ]
    GeneIDi 825399.
    KEGGi ath:AT3G62260.

    Organism-specific databases

    TAIRi AT3G62260.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000233896.
    InParanoidi Q3EAF9.
    KOi K14803.
    OMAi VMTSQHA.
    PhylomeDBi Q3EAF9.

    Enzyme and pathway databases

    BioCyci ARA:AT3G62260-MONOMER.
    ARA:GQT-2642-MONOMER.

    Gene expression databases

    Genevestigatori Q3EAF9.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.
      Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C49_ARATH
    AccessioniPrimary (citable) accession number: Q3EAF9
    Secondary accession number(s): Q9M1P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3