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Q3EAF9

- P2C49_ARATH

UniProt

Q3EAF9 - P2C49_ARATH

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Protein

Probable protein phosphatase 2C 49

Gene

At3g62260

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Manganese 1By similarity
Metal bindingi122 – 1221Manganese 2By similarity
Metal bindingi123 – 1231Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi297 – 2971Manganese 2By similarity
Metal bindingi340 – 3401Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G62260-MONOMER.
ARA:GQT-2642-MONOMER.
ReactomeiREACT_247278. ISG15 antiviral mechanism.
REACT_259981. Regulation of AMPK activity via LKB1.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 49 (EC:3.1.3.16)
Short name:
AtPP2C49
Gene namesi
Ordered Locus Names:At3g62260
ORF Names:T17J13.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G62260.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Probable protein phosphatase 2C 49PRO_0000367973Add
BLAST

Proteomic databases

PRIDEiQ3EAF9.

Expressioni

Gene expression databases

GenevestigatoriQ3EAF9.

Interactioni

Protein-protein interaction databases

BioGridi10713. 3 interactions.
IntActiQ3EAF9. 1 interaction.
STRINGi3702.AT3G62260.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ3EAF9.
SMRiQ3EAF9. Positions 77-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 342266PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ3EAF9.
KOiK14803.
OMAiVMTSQHA.
PhylomeDBiQ3EAF9.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3EAF9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAEAEVVFQ QSLPAVLEIE LFDGVSSAVK SPVSSPKLGF TQSTASVSGS
60 70 80 90 100
LTTSPVQADI FPEGDCDPSV LDYIPTIRSG SFADIGPKRN MEDEHIRIDD
110 120 130 140 150
LSSQVGSLFE LPKPSAFYAV FDGHGGPEAA AYVRENAIRF FFEDEQFPQT
160 170 180 190 200
SEVSSVYVEE VETSLRNAFL QADLALAEDC SISDSCGTTA LTALICGRLL
210 220 230 240 250
MVANAGDCRA VLCRKGRAID MSEDHKPINL LERRRVEESG GFITNDGYLN
260 270 280 290 300
EVLAVTRALG DWDLKLPHGS QSPLISEPEI KQITLTEDDE FLVIGCDGIW
310 320 330 340 350
DVLTSQEAVS IVRRGLNRHN DPTRCARELV MEALGRNSFD NLTAVVVCFM
360 370 380
TMDRGDKPVV PLEKRRCFSL SPEAFCSLRN LLDG

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Length:384
Mass (Da):41,968
Last modified:November 8, 2005 - v1
Checksum:i70C776A812AED76A
GO
Isoform 2 (identifier: Q3EAF9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     57-57: Missing.

Show »
Length:383
Mass (Da):41,840
Checksum:i3ED36648FF529C0A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei57 – 571Missing in isoform 2. 3 PublicationsVSP_036774

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138651 Genomic DNA. Translation: CAB71886.1.
CP002686 Genomic DNA. Translation: AEE80328.1.
CP002686 Genomic DNA. Translation: AEE80329.1.
AK316866 mRNA. Translation: BAH19574.1.
BT030025 mRNA. Translation: ABN04763.1.
AY085949 mRNA. Translation: AAM63159.1.
PIRiT48018.
RefSeqiNP_191785.1. NM_116091.3. [Q3EAF9-2]
NP_850737.1. NM_180406.1. [Q3EAF9-1]
UniGeneiAt.43534.

Genome annotation databases

EnsemblPlantsiAT3G62260.2; AT3G62260.2; AT3G62260. [Q3EAF9-1]
GeneIDi825399.
KEGGiath:AT3G62260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138651 Genomic DNA. Translation: CAB71886.1 .
CP002686 Genomic DNA. Translation: AEE80328.1 .
CP002686 Genomic DNA. Translation: AEE80329.1 .
AK316866 mRNA. Translation: BAH19574.1 .
BT030025 mRNA. Translation: ABN04763.1 .
AY085949 mRNA. Translation: AAM63159.1 .
PIRi T48018.
RefSeqi NP_191785.1. NM_116091.3. [Q3EAF9-2 ]
NP_850737.1. NM_180406.1. [Q3EAF9-1 ]
UniGenei At.43534.

3D structure databases

ProteinModelPortali Q3EAF9.
SMRi Q3EAF9. Positions 77-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 10713. 3 interactions.
IntActi Q3EAF9. 1 interaction.
STRINGi 3702.AT3G62260.2-P.

Proteomic databases

PRIDEi Q3EAF9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G62260.2 ; AT3G62260.2 ; AT3G62260 . [Q3EAF9-1 ]
GeneIDi 825399.
KEGGi ath:AT3G62260.

Organism-specific databases

TAIRi AT3G62260.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000233896.
InParanoidi Q3EAF9.
KOi K14803.
OMAi VMTSQHA.
PhylomeDBi Q3EAF9.

Enzyme and pathway databases

BioCyci ARA:AT3G62260-MONOMER.
ARA:GQT-2642-MONOMER.
Reactomei REACT_247278. ISG15 antiviral mechanism.
REACT_259981. Regulation of AMPK activity via LKB1.

Gene expression databases

Genevestigatori Q3EAF9.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.
    Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C49_ARATH
AccessioniPrimary (citable) accession number: Q3EAF9
Secondary accession number(s): Q9M1P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 8, 2005
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3