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Q3EAF9 (P2C49_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable protein phosphatase 2C 49
Short name=AtPP2C49
EC=3.1.3.16
Gene names
Ordered Locus Names:At3g62260
ORF Names:T17J13.220
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein dephosphorylation

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine phosphatase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3EAF9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.
Isoform 2 (identifier: Q3EAF9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     57-57: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Probable protein phosphatase 2C 49
PRO_0000367973

Regions

Domain77 – 342266PP2C-like

Sites

Metal binding1221Manganese 1 By similarity
Metal binding1221Manganese 2 By similarity
Metal binding1231Manganese 1; via carbonyl oxygen By similarity
Metal binding2971Manganese 2 By similarity
Metal binding3401Manganese 2 By similarity

Natural variations

Alternative sequence571Missing in isoform 2.
VSP_036774

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 70C776A812AED76A

FASTA38441,968
        10         20         30         40         50         60 
MVAEAEVVFQ QSLPAVLEIE LFDGVSSAVK SPVSSPKLGF TQSTASVSGS LTTSPVQADI 

        70         80         90        100        110        120 
FPEGDCDPSV LDYIPTIRSG SFADIGPKRN MEDEHIRIDD LSSQVGSLFE LPKPSAFYAV 

       130        140        150        160        170        180 
FDGHGGPEAA AYVRENAIRF FFEDEQFPQT SEVSSVYVEE VETSLRNAFL QADLALAEDC 

       190        200        210        220        230        240 
SISDSCGTTA LTALICGRLL MVANAGDCRA VLCRKGRAID MSEDHKPINL LERRRVEESG 

       250        260        270        280        290        300 
GFITNDGYLN EVLAVTRALG DWDLKLPHGS QSPLISEPEI KQITLTEDDE FLVIGCDGIW 

       310        320        330        340        350        360 
DVLTSQEAVS IVRRGLNRHN DPTRCARELV MEALGRNSFD NLTAVVVCFM TMDRGDKPVV 

       370        380 
PLEKRRCFSL SPEAFCSLRN LLDG

« Hide

Isoform 2 [UniParc].

Checksum: 3ED36648FF529C0A
Show »

FASTA38341,840

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL138651 Genomic DNA. Translation: CAB71886.1.
CP002686 Genomic DNA. Translation: AEE80328.1.
CP002686 Genomic DNA. Translation: AEE80329.1.
AK316866 mRNA. Translation: BAH19574.1.
BT030025 mRNA. Translation: ABN04763.1.
AY085949 mRNA. Translation: AAM63159.1.
IPIIPI00532443.
IPI00532568.
PIRT48018.
RefSeqNP_191785.1. NM_116091.3.
NP_850737.1. NM_180406.1.
UniGeneAt.43534.

3D structure databases

ProteinModelPortalQ3EAF9.
SMRQ3EAF9. Positions 77-349.
ModBaseSearch...

Protein-protein interaction databases

IntActQ3EAF9. 1 interaction.
STRING3702.AT3G62260.2-P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G62260.2; AT3G62260.2; AT3G62260.
GeneID825399.
KEGGath:AT3G62260.

Organism-specific databases

TAIRAt3g62260.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233896.
InParanoidQ3EAF9.
KOK14803.
OMACARELVM.
PhylomeDBQ3EAF9.
ProtClustDBCLSN2684124.

Gene expression databases

GenevestigatorQ3EAF9.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. PP2C-related. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C49_ARATH
AccessionPrimary (citable) accession number: Q3EAF9
Secondary accession number(s): Q9M1P8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 8, 2005
Last modified: May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents