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Protein

Calcium-dependent protein kinase 34

Gene

CPK34

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97ATPPROSITE-ProRule annotation1
Active sitei192Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 82ATPPROSITE-ProRule annotation9
Calcium bindingi382 – 3931PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi418 – 4292PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi454 – 4653PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi489 – 5004PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371571 HSF1-dependent transactivation
R-ATH-4086398 Ca2+ pathway
R-ATH-442729 CREB phosphorylation through the activation of CaMKII
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 34 (EC:2.7.11.1)
Gene namesi
Name:CPK34
Ordered Locus Names:At5g19360
ORF Names:F7K24.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G19360
TAIRilocus:2150225 AT5G19360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00003633552 – 523Calcium-dependent protein kinase 34Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei232PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ3E9C0

PTM databases

iPTMnetiQ3E9C0

Expressioni

Gene expression databases

ExpressionAtlasiQ3E9C0 baseline and differential
GenevisibleiQ3E9C0 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G19360.1

Structurei

3D structure databases

ProteinModelPortaliQ3E9C0
SMRiQ3E9C0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 326Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini369 – 404EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini405 – 440EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini441 – 476EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini477 – 511EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni332 – 362Autoinhibitory domainBy similarityAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 28Poly-Gly6

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (332-362) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
HOGENOMiHOG000233030
InParanoidiQ3E9C0
KOiK13412
OMAiTFEDHKN
OrthoDBiEOG093609AJ
PhylomeDBiQ3E9C0

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3E9C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCCSHGRD SDDNKEEPRP ENGGGGVGAA EASVRASKHP PASPPPATKQ
60 70 80 90 100
GPIGPVLGRP MEDVKSSYTL GKELGRGQFG VTHLCTQKAT GLQFACKTIA
110 120 130 140 150
KRKLVNKEDI EDVRREVQIM HHLTGQPNIV ELKGAYEDKH SVHLVMELCA
160 170 180 190 200
GGELFDRIIA KGHYSERAAA SLLRTIVQII HTCHSMGVIH RDLKPENFLL
210 220 230 240 250
LSKDENSPLK ATDFGLSVFY KPGEVFKDIV GSAYYIAPEV LRRKYGPEAD
260 270 280 290 300
IWSIGVMLYI LLCGVPPFWA ESENGIFNAI LSGQVDFSSD PWPVISPQAK
310 320 330 340 350
DLVRKMLNSD PKQRLTAAQV LNHPWIKEDG EAPDVPLDNA VMSRLKQFKA
360 370 380 390 400
MNNFKKVALR VIAGCLSEEE IMGLKEMFKG MDTDNSGTIT LEELRQGLAK
410 420 430 440 450
QGTRLSEYEV QQLMEAADAD GNGTIDYGEF IAATMHINRL DREEHLYSAF
460 470 480 490 500
QHFDKDNSGY ITTEELEQAL REFGMNDGRD IKEIISEVDG DNDGRINYEE
510 520
FVAMMRKGNP DPNPKKRREL SFK
Length:523
Mass (Da):58,174
Last modified:November 8, 2005 - v1
Checksum:i57E26A3A385FB168
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296837 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92691.1
DQ446970 mRNA Translation: ABE66169.1
RefSeqiNP_197437.1, NM_121941.2
UniGeneiAt.54915

Genome annotation databases

EnsemblPlantsiAT5G19360.1; AT5G19360.1; AT5G19360
GeneIDi832056
GrameneiAT5G19360.1; AT5G19360.1; AT5G19360
KEGGiath:AT5G19360

Similar proteinsi

Entry informationi

Entry nameiCDPKY_ARATH
AccessioniPrimary (citable) accession number: Q3E9C0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 8, 2005
Last modified: April 25, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome