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Protein

Calcium-dependent protein kinase 34

Gene

CPK34

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97ATPPROSITE-ProRule annotation1
Active sitei192Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 82ATPPROSITE-ProRule annotation9
Calcium bindingi382 – 3931PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi418 – 4292PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi454 – 4653PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi489 – 5004PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 34 (EC:2.7.11.1)
Gene namesi
Name:CPK34
Ordered Locus Names:At5g19360
ORF Names:F7K24.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19360.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00003633552 – 523Calcium-dependent protein kinase 34Add BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ3E9C0.

PTM databases

iPTMnetiQ3E9C0.

Expressioni

Gene expression databases

GenevisibleiQ3E9C0. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G19360.1.

Structurei

3D structure databases

ProteinModelPortaliQ3E9C0.
SMRiQ3E9C0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 326Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini369 – 404EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini405 – 440EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini441 – 476EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini477 – 511EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni332 – 362Autoinhibitory domainBy similarityAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 28Poly-Gly6

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (332-362) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ3E9C0.
KOiK13412.
OMAiYKQGTHA.
OrthoDBiEOG093609AJ.
PhylomeDBiQ3E9C0.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3E9C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCCSHGRD SDDNKEEPRP ENGGGGVGAA EASVRASKHP PASPPPATKQ
60 70 80 90 100
GPIGPVLGRP MEDVKSSYTL GKELGRGQFG VTHLCTQKAT GLQFACKTIA
110 120 130 140 150
KRKLVNKEDI EDVRREVQIM HHLTGQPNIV ELKGAYEDKH SVHLVMELCA
160 170 180 190 200
GGELFDRIIA KGHYSERAAA SLLRTIVQII HTCHSMGVIH RDLKPENFLL
210 220 230 240 250
LSKDENSPLK ATDFGLSVFY KPGEVFKDIV GSAYYIAPEV LRRKYGPEAD
260 270 280 290 300
IWSIGVMLYI LLCGVPPFWA ESENGIFNAI LSGQVDFSSD PWPVISPQAK
310 320 330 340 350
DLVRKMLNSD PKQRLTAAQV LNHPWIKEDG EAPDVPLDNA VMSRLKQFKA
360 370 380 390 400
MNNFKKVALR VIAGCLSEEE IMGLKEMFKG MDTDNSGTIT LEELRQGLAK
410 420 430 440 450
QGTRLSEYEV QQLMEAADAD GNGTIDYGEF IAATMHINRL DREEHLYSAF
460 470 480 490 500
QHFDKDNSGY ITTEELEQAL REFGMNDGRD IKEIISEVDG DNDGRINYEE
510 520
FVAMMRKGNP DPNPKKRREL SFK
Length:523
Mass (Da):58,174
Last modified:November 8, 2005 - v1
Checksum:i57E26A3A385FB168
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296837 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92691.1.
DQ446970 mRNA. Translation: ABE66169.1.
RefSeqiNP_197437.1. NM_121941.2.
UniGeneiAt.54915.

Genome annotation databases

EnsemblPlantsiAT5G19360.1; AT5G19360.1; AT5G19360.
GeneIDi832056.
GrameneiAT5G19360.1; AT5G19360.1; AT5G19360.
KEGGiath:AT5G19360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296837 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92691.1.
DQ446970 mRNA. Translation: ABE66169.1.
RefSeqiNP_197437.1. NM_121941.2.
UniGeneiAt.54915.

3D structure databases

ProteinModelPortaliQ3E9C0.
SMRiQ3E9C0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G19360.1.

PTM databases

iPTMnetiQ3E9C0.

Proteomic databases

PaxDbiQ3E9C0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19360.1; AT5G19360.1; AT5G19360.
GeneIDi832056.
GrameneiAT5G19360.1; AT5G19360.1; AT5G19360.
KEGGiath:AT5G19360.

Organism-specific databases

TAIRiAT5G19360.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ3E9C0.
KOiK13412.
OMAiYKQGTHA.
OrthoDBiEOG093609AJ.
PhylomeDBiQ3E9C0.

Miscellaneous databases

PROiQ3E9C0.

Gene expression databases

GenevisibleiQ3E9C0. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPKY_ARATH
AccessioniPrimary (citable) accession number: Q3E9C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 8, 2005
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.