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Protein

Putative myrosinase 3

Gene

TGG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein uncertaini

Functioni

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145SubstrateBy similarity1
Binding sitei190SubstrateBy similarity1
Binding sitei191AscorbateBy similarity1
Binding sitei246AscorbateBy similarity1
Binding sitei316SubstrateBy similarity1
Active sitei386NucleophileBy similarity1
Binding sitei404SubstrateBy similarity1

GO - Molecular functioni

  • beta-glucosidase activity Source: GO_Central
  • thioglucosidase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G48375-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative myrosinase 3 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 39
Short name:
AtBGLU39
Sinigrinase 3
Thioglucosidase 3
Gene namesi
Name:TGG3
Synonyms:BGLU39
Ordered Locus Names:At5g48375
ORF Names:K23F3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G48375.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000039031220 – 439Putative myrosinase 3Add BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Glycosylationi336N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3E8E5.
PRIDEiQ3E8E5.

Expressioni

Tissue specificityi

Expressed specifically in stamens and petals.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi20137. 1 interactor.
STRINGi3702.AT5G48375.1.

Structurei

3D structure databases

ProteinModelPortaliQ3E8E5.
SMRiQ3E8E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni411 – 412Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ3E8E5.
KOiK01237.
OMAiEITCEET.
PhylomeDBiQ3E8E5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3E8E5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFRALGLVL LLAVETCKAE EITCEETKPF TCNQTDRFNR KHFDDDFIFE
60 70 80 90 100
GGKGRGLNVW DGFTHRYPEK GGPDLGNGDS TCGSYEHWQK DIDVMTELGV
110 120 130 140 150
DGYRFSLAWS RIAPRESNQA GVKYYNDLID GLLAKNITPF VTLFHWDLPQ
160 170 180 190 200
VLQDEYEGFL NHEIIDDFKD YANLCFKIFG DRVKKWITIN QLYTVPTRGY
210 220 230 240 250
AMGTDAPEPY IVAHNQLLAH AKVVHLYRKK YKPKQRGQIG VVMITRWFVP
260 270 280 290 300
YDSTQANIDA TERNKEFFLG WFMEPLTKGK YPDIMRKLVG RRLPKFNKKE
310 320 330 340 350
AKLVKGSYDF LGINYYQTQY VYAIPANPPN RLTVLNDSLS AFSYENKDGP
360 370 380 390 400
IGPWFNADSY YHPRGILNVL EHFKTKYGNP LVYITENGEL LILSGCNVKG
410 420 430
YFAWCLGDNY ELWPSRSFHV SPFYLLHRKD KGAFPSFEA
Length:439
Mass (Da):50,758
Last modified:November 8, 2005 - v1
Checksum:i89D2ADBD5024184E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000372 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED95661.1.
RefSeqiNP_680406.1. NM_148101.1.
UniGeneiAt.55430.

Genome annotation databases

EnsemblPlantsiAT5G48375.1; AT5G48375.1; AT5G48375.
GeneIDi834891.
GrameneiAT5G48375.1; AT5G48375.1; AT5G48375.
KEGGiath:AT5G48375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000372 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED95661.1.
RefSeqiNP_680406.1. NM_148101.1.
UniGeneiAt.55430.

3D structure databases

ProteinModelPortaliQ3E8E5.
SMRiQ3E8E5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20137. 1 interactor.
STRINGi3702.AT5G48375.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ3E8E5.
PRIDEiQ3E8E5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G48375.1; AT5G48375.1; AT5G48375.
GeneIDi834891.
GrameneiAT5G48375.1; AT5G48375.1; AT5G48375.
KEGGiath:AT5G48375.

Organism-specific databases

TAIRiAT5G48375.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ3E8E5.
KOiK01237.
OMAiEITCEET.
PhylomeDBiQ3E8E5.

Enzyme and pathway databases

BioCyciARA:AT5G48375-MONOMER.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGL39_ARATH
AccessioniPrimary (citable) accession number: Q3E8E5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Could be the product of a pseudogene.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.