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Q3E8E5

- BGL39_ARATH

UniProt

Q3E8E5 - BGL39_ARATH

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Protein

Putative myrosinase 3

Gene

TGG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini

Functioni

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 1451SubstrateBy similarity
Binding sitei190 – 1901SubstrateBy similarity
Binding sitei191 – 1911AscorbateBy similarity
Binding sitei246 – 2461AscorbateBy similarity
Binding sitei316 – 3161SubstrateBy similarity
Active sitei386 – 3861NucleophileBy similarity
Binding sitei404 – 4041SubstrateBy similarity

GO - Molecular functioni

  1. thioglucosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G48375-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative myrosinase 3 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 39
Short name:
AtBGLU39
Sinigrinase 3
Thioglucosidase 3
Gene namesi
Name:TGG3
Synonyms:BGLU39
Ordered Locus Names:At5g48375
ORF Names:K23F3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G48375.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 439420Putative myrosinase 3PRO_0000390312Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3E8E5.
PRIDEiQ3E8E5.

Expressioni

Tissue specificityi

Expressed specifically in stamens and petals.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G48375.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ3E8E5.
SMRiQ3E8E5. Positions 21-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni411 – 4122Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ3E8E5.
KOiK01237.
OMAiEITCEET.
PhylomeDBiQ3E8E5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3E8E5 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFRALGLVL LLAVETCKAE EITCEETKPF TCNQTDRFNR KHFDDDFIFE
60 70 80 90 100
GGKGRGLNVW DGFTHRYPEK GGPDLGNGDS TCGSYEHWQK DIDVMTELGV
110 120 130 140 150
DGYRFSLAWS RIAPRESNQA GVKYYNDLID GLLAKNITPF VTLFHWDLPQ
160 170 180 190 200
VLQDEYEGFL NHEIIDDFKD YANLCFKIFG DRVKKWITIN QLYTVPTRGY
210 220 230 240 250
AMGTDAPEPY IVAHNQLLAH AKVVHLYRKK YKPKQRGQIG VVMITRWFVP
260 270 280 290 300
YDSTQANIDA TERNKEFFLG WFMEPLTKGK YPDIMRKLVG RRLPKFNKKE
310 320 330 340 350
AKLVKGSYDF LGINYYQTQY VYAIPANPPN RLTVLNDSLS AFSYENKDGP
360 370 380 390 400
IGPWFNADSY YHPRGILNVL EHFKTKYGNP LVYITENGEL LILSGCNVKG
410 420 430
YFAWCLGDNY ELWPSRSFHV SPFYLLHRKD KGAFPSFEA
Length:439
Mass (Da):50,758
Last modified:November 8, 2005 - v1
Checksum:i89D2ADBD5024184E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP000372 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED95661.1.
RefSeqiNP_680406.1. NM_148101.1.
UniGeneiAt.55430.

Genome annotation databases

EnsemblPlantsiAT5G48375.1; AT5G48375.1; AT5G48375.
GeneIDi834891.
KEGGiath:AT5G48375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP000372 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED95661.1 .
RefSeqi NP_680406.1. NM_148101.1.
UniGenei At.55430.

3D structure databases

ProteinModelPortali Q3E8E5.
SMRi Q3E8E5. Positions 21-412.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G48375.1-P.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi Q3E8E5.
PRIDEi Q3E8E5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G48375.1 ; AT5G48375.1 ; AT5G48375 .
GeneIDi 834891.
KEGGi ath:AT5G48375.

Organism-specific databases

TAIRi AT5G48375.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi Q3E8E5.
KOi K01237.
OMAi EITCEET.
PhylomeDBi Q3E8E5.

Enzyme and pathway databases

BioCyci ARA:AT5G48375-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "The third myrosinase gene TGG3 in Arabidopsis thaliana is a pseudogene specifically expressed in stamen and petal."
    Zhang J., Pontoppidan B., Xue J., Rask L., Meijer J.
    Physiol. Plantarum 115:25-34(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL39_ARATH
AccessioniPrimary (citable) accession number: Q3E8E5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Could be the product of a pseudogene.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3