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Q3E8E5 (BGL39_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative myrosinase 3

EC=3.2.1.147
Alternative name(s):
Beta-glucosidase 39
Short name=AtBGLU39
Sinigrinase 3
Thioglucosidase 3
Gene names
Name:TGG3
Synonyms:BGLU39
Ordered Locus Names:At5g48375
ORF Names:K23F3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceUncertain

General annotation (Comments)

Catalytic activity

A thioglucoside + H2O = a sugar + a thiol.

Tissue specificity

Expressed specifically in stamens and petals. Ref.3

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Caution

Could be the product of a pseudogene.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionthioglucosidase activity

Inferred from direct assay Ref.3. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 439420Putative myrosinase 3
PRO_0000390312

Regions

Region411 – 4122Substrate binding By similarity

Sites

Active site3861Nucleophile By similarity
Binding site1451Substrate By similarity
Binding site1901Substrate By similarity
Binding site1911Ascorbate By similarity
Binding site2461Ascorbate By similarity
Binding site3161Substrate By similarity
Binding site4041Substrate By similarity

Amino acid modifications

Glycosylation331N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q3E8E5 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 89D2ADBD5024184E

FASTA43950,758
        10         20         30         40         50         60 
MKFRALGLVL LLAVETCKAE EITCEETKPF TCNQTDRFNR KHFDDDFIFE GGKGRGLNVW 

        70         80         90        100        110        120 
DGFTHRYPEK GGPDLGNGDS TCGSYEHWQK DIDVMTELGV DGYRFSLAWS RIAPRESNQA 

       130        140        150        160        170        180 
GVKYYNDLID GLLAKNITPF VTLFHWDLPQ VLQDEYEGFL NHEIIDDFKD YANLCFKIFG 

       190        200        210        220        230        240 
DRVKKWITIN QLYTVPTRGY AMGTDAPEPY IVAHNQLLAH AKVVHLYRKK YKPKQRGQIG 

       250        260        270        280        290        300 
VVMITRWFVP YDSTQANIDA TERNKEFFLG WFMEPLTKGK YPDIMRKLVG RRLPKFNKKE 

       310        320        330        340        350        360 
AKLVKGSYDF LGINYYQTQY VYAIPANPPN RLTVLNDSLS AFSYENKDGP IGPWFNADSY 

       370        380        390        400        410        420 
YHPRGILNVL EHFKTKYGNP LVYITENGEL LILSGCNVKG YFAWCLGDNY ELWPSRSFHV 

       430 
SPFYLLHRKD KGAFPSFEA 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"The third myrosinase gene TGG3 in Arabidopsis thaliana is a pseudogene specifically expressed in stamen and petal."
Zhang J., Pontoppidan B., Xue J., Rask L., Meijer J.
Physiol. Plantarum 115:25-34(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000372 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED95661.1.
RefSeqNP_680406.1. NM_148101.1.
UniGeneAt.55430.

3D structure databases

ProteinModelPortalQ3E8E5.
SMRQ3E8E5. Positions 21-435.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G48375.1-P.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbQ3E8E5.
PRIDEQ3E8E5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G48375.1; AT5G48375.1; AT5G48375.
GeneID834891.
KEGGath:AT5G48375.

Organism-specific databases

TAIRAT5G48375.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidQ3E8E5.
KOK01237.
OMAEITCEET.
PhylomeDBQ3E8E5.

Enzyme and pathway databases

BioCycARA:AT5G48375-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGL39_ARATH
AccessionPrimary (citable) accession number: Q3E8E5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names