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Q3E8E5

- BGL39_ARATH

UniProt

Q3E8E5 - BGL39_ARATH

Protein

Putative myrosinase 3

Gene

TGG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A thioglucoside + H2O = a sugar + a thiol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451SubstrateBy similarity
    Binding sitei190 – 1901SubstrateBy similarity
    Binding sitei191 – 1911AscorbateBy similarity
    Binding sitei246 – 2461AscorbateBy similarity
    Binding sitei316 – 3161SubstrateBy similarity
    Active sitei386 – 3861NucleophileBy similarity
    Binding sitei404 – 4041SubstrateBy similarity

    GO - Molecular functioni

    1. thioglucosidase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G48375-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative myrosinase 3 (EC:3.2.1.147)
    Alternative name(s):
    Beta-glucosidase 39
    Short name:
    AtBGLU39
    Sinigrinase 3
    Thioglucosidase 3
    Gene namesi
    Name:TGG3
    Synonyms:BGLU39
    Ordered Locus Names:At5g48375
    ORF Names:K23F3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G48375.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 439420Putative myrosinase 3PRO_0000390312Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ3E8E5.
    PRIDEiQ3E8E5.

    Expressioni

    Tissue specificityi

    Expressed specifically in stamens and petals.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G48375.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3E8E5.
    SMRiQ3E8E5. Positions 21-435.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni411 – 4122Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiQ3E8E5.
    KOiK01237.
    OMAiEITCEET.
    PhylomeDBiQ3E8E5.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3E8E5-1 [UniParc]FASTAAdd to Basket

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    MKFRALGLVL LLAVETCKAE EITCEETKPF TCNQTDRFNR KHFDDDFIFE    50
    GGKGRGLNVW DGFTHRYPEK GGPDLGNGDS TCGSYEHWQK DIDVMTELGV 100
    DGYRFSLAWS RIAPRESNQA GVKYYNDLID GLLAKNITPF VTLFHWDLPQ 150
    VLQDEYEGFL NHEIIDDFKD YANLCFKIFG DRVKKWITIN QLYTVPTRGY 200
    AMGTDAPEPY IVAHNQLLAH AKVVHLYRKK YKPKQRGQIG VVMITRWFVP 250
    YDSTQANIDA TERNKEFFLG WFMEPLTKGK YPDIMRKLVG RRLPKFNKKE 300
    AKLVKGSYDF LGINYYQTQY VYAIPANPPN RLTVLNDSLS AFSYENKDGP 350
    IGPWFNADSY YHPRGILNVL EHFKTKYGNP LVYITENGEL LILSGCNVKG 400
    YFAWCLGDNY ELWPSRSFHV SPFYLLHRKD KGAFPSFEA 439
    Length:439
    Mass (Da):50,758
    Last modified:November 8, 2005 - v1
    Checksum:i89D2ADBD5024184E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000372 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED95661.1.
    RefSeqiNP_680406.1. NM_148101.1.
    UniGeneiAt.55430.

    Genome annotation databases

    EnsemblPlantsiAT5G48375.1; AT5G48375.1; AT5G48375.
    GeneIDi834891.
    KEGGiath:AT5G48375.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000372 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED95661.1 .
    RefSeqi NP_680406.1. NM_148101.1.
    UniGenei At.55430.

    3D structure databases

    ProteinModelPortali Q3E8E5.
    SMRi Q3E8E5. Positions 21-435.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G48375.1-P.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi Q3E8E5.
    PRIDEi Q3E8E5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G48375.1 ; AT5G48375.1 ; AT5G48375 .
    GeneIDi 834891.
    KEGGi ath:AT5G48375.

    Organism-specific databases

    TAIRi AT5G48375.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    InParanoidi Q3E8E5.
    KOi K01237.
    OMAi EITCEET.
    PhylomeDBi Q3E8E5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G48375-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
      Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "The third myrosinase gene TGG3 in Arabidopsis thaliana is a pseudogene specifically expressed in stamen and petal."
      Zhang J., Pontoppidan B., Xue J., Rask L., Meijer J.
      Physiol. Plantarum 115:25-34(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL39_ARATH
    AccessioniPrimary (citable) accession number: Q3E8E5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Could be the product of a pseudogene.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3