Q3E8E5 (BGL39_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative myrosinase 3
EC=3.2.1.147

Alternative name(s):
Beta-glucosidase 39
Short name=AtBGLU39
Sinigrinase 3
Thioglucosidase 3

Gene names

Name:	TGG3
Synonyms:	BGLU39
Ordered Locus Names:	At5g48375
ORF Names:	K23F3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Uncertain

General annotation (Comments)

Catalytic activity	A thioglucoside + H2O = a sugar + a thiol.
Tissue specificity	Expressed specifically in stamens and petals. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Caution	Could be the product of a pseudogene.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	thioglucosidase activity Inferred from direct assay Ref.3. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 439	420	Putative myrosinase 3		PRO_0000390312
Regions
	Region	411 – 412	2	Substrate binding By similarity
Sites
	Active site	386	1	Nucleophile By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	191	1	Ascorbate By similarity
	Binding site	246	1	Ascorbate By similarity
	Binding site	316	1	Substrate By similarity
	Binding site	404	1	Substrate By similarity
Amino acid modifications
	Glycosylation	33	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3E8E5 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 89D2ADBD5024184E	FASTA	439	50,758
	10 20 30 40 50 60 MKFRALGLVL LLAVETCKAE EITCEETKPF TCNQTDRFNR KHFDDDFIFE GGKGRGLNVW 70 80 90 100 110 120 DGFTHRYPEK GGPDLGNGDS TCGSYEHWQK DIDVMTELGV DGYRFSLAWS RIAPRESNQA 130 140 150 160 170 180 GVKYYNDLID GLLAKNITPF VTLFHWDLPQ VLQDEYEGFL NHEIIDDFKD YANLCFKIFG 190 200 210 220 230 240 DRVKKWITIN QLYTVPTRGY AMGTDAPEPY IVAHNQLLAH AKVVHLYRKK YKPKQRGQIG 250 260 270 280 290 300 VVMITRWFVP YDSTQANIDA TERNKEFFLG WFMEPLTKGK YPDIMRKLVG RRLPKFNKKE 310 320 330 340 350 360 AKLVKGSYDF LGINYYQTQY VYAIPANPPN RLTVLNDSLS AFSYENKDGP IGPWFNADSY 370 380 390 400 410 420 YHPRGILNVL EHFKTKYGNP LVYITENGEL LILSGCNVKG YFAWCLGDNY ELWPSRSFHV 430 SPFYLLHRKD KGAFPSFEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"The third myrosinase gene TGG3 in Arabidopsis thaliana is a pseudogene specifically expressed in stamen and petal." Zhang J., Pontoppidan B., Xue J., Rask L., Meijer J. Physiol. Plantarum 115:25-34(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1." Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A. Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000372 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED95661.1.
IPI	IPI00520195.
RefSeq	NP_680406.1. NM_148101.1.
UniGene	At.55430.
3D structure databases
ProteinModelPortal	Q3E8E5.
SMR	Q3E8E5. Positions 21-412.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G48375.1-P.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Proteomic databases
PaxDb	Q3E8E5.
PRIDE	Q3E8E5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G48375.1; AT5G48375.1; AT5G48375.
GeneID	834891.
KEGG	ath:AT5G48375.
Organism-specific databases
TAIR	At5g48375.
Phylogenomic databases
eggNOG	COG2723.
HOGENOM	HOG000088630.
InParanoid	Q3E8E5.
OMA	EITCEET.
PhylomeDB	Q3E8E5.
ProtClustDB	CLSN2689871.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGL39_ARATH

Accession

Primary (citable) accession number: Q3E8E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program