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Protein

Diphthamide biosynthesis protein 3

Gene

KTI11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2. Required for conferring sensitivity to K.lactis zymocin.1 Publication

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5957DPH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • electron carrier activity Source: SGD
  • iron ion binding Source: SGD
  • zinc ion binding Source: SGD

GO - Biological processi

  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: SGD
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15580.
YEAST:MONOMER-15580.
ReactomeiR-SCE-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthamide biosynthesis protein 3
Alternative name(s):
Kluyveromyces lactis toxin-insensitive protein 11
Gene namesi
Name:KTI11
Synonyms:DPH3
Ordered Locus Names:YBL071W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL071W-A.
SGDiS000007587. KTI11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8282Diphthamide biosynthesis protein 3PRO_0000082639Add
BLAST

Proteomic databases

MaxQBiQ3E840.

Interactioni

Subunit structurei

Interacts with the diphthamide biosynthesis proteins DPH1 and DPH2. Interacts with elongation factor 2 (EFT2), the core-elongator subunits IKI3/ELP1, ELP2 and ELP3, and the 40S ribosomal proteins RPS7A and RPS19A.1 Publication

Protein-protein interaction databases

BioGridi32630. 21 interactions.
DIPiDIP-52225N.
IntActiQ3E840. 4 interactions.
MINTiMINT-2786415.

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Helixi9 – 113Combined sources
Beta strandi12 – 154Combined sources
Turni16 – 194Combined sources
Beta strandi20 – 245Combined sources
Beta strandi28 – 347Combined sources
Helixi35 – 395Combined sources
Beta strandi44 – 463Combined sources
Beta strandi48 – 514Combined sources
Beta strandi53 – 575Combined sources
Helixi60 – 7011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YOPNMR-A1-82[»]
1YWSNMR-A1-82[»]
4D4OX-ray2.90A/B/C1-82[»]
4D4PX-ray3.00A/B/C/E/G/H1-82[»]
4X33X-ray1.45A1-57[»]
ProteinModelPortaliQ3E840.
SMRiQ3E840. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3E840.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi79 – 824Poly-Ala

Sequence similaritiesi

Belongs to the DPH3 family.Curated
Contains 1 DPH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5957DPH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000007225.
HOGENOMiHOG000190740.
InParanoidiQ3E840.
KOiK15455.
OMAiREMLEMG.
OrthoDBiEOG092C5WZE.

Family and domain databases

InterProiIPR007872. Znf_DHP.
[Graphical view]
PfamiPF05207. zf-CSL. 1 hit.
[Graphical view]
PROSITEiPS51074. ZF_DPH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3E840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTYDEIEIE DMTFEPENQM FTYPCPCGDR FQIYLDDMFE GEKVAVCPSC
60 70 80
SLMIDVVFDK EDLAEYYEEA GIHPPEPIAA AA
Length:82
Mass (Da):9,356
Last modified:November 8, 2005 - v1
Checksum:i8B27A307831B0ECE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35832 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07051.1.
RefSeqiNP_660100.1. NM_001184454.1.

Genome annotation databases

EnsemblFungiiYBL071W-A; YBL071W-A; YBL071W-A.
GeneIDi852207.
KEGGisce:YBL071W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35832 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07051.1.
RefSeqiNP_660100.1. NM_001184454.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YOPNMR-A1-82[»]
1YWSNMR-A1-82[»]
4D4OX-ray2.90A/B/C1-82[»]
4D4PX-ray3.00A/B/C/E/G/H1-82[»]
4X33X-ray1.45A1-57[»]
ProteinModelPortaliQ3E840.
SMRiQ3E840. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32630. 21 interactions.
DIPiDIP-52225N.
IntActiQ3E840. 4 interactions.
MINTiMINT-2786415.

Proteomic databases

MaxQBiQ3E840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL071W-A; YBL071W-A; YBL071W-A.
GeneIDi852207.
KEGGisce:YBL071W-A.

Organism-specific databases

EuPathDBiFungiDB:YBL071W-A.
SGDiS000007587. KTI11.

Phylogenomic databases

GeneTreeiENSGT00390000007225.
HOGENOMiHOG000190740.
InParanoidiQ3E840.
KOiK15455.
OMAiREMLEMG.
OrthoDBiEOG092C5WZE.

Enzyme and pathway databases

UniPathwayiUPA00559.
BioCyciMetaCyc:MONOMER-15580.
YEAST:MONOMER-15580.
ReactomeiR-SCE-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

EvolutionaryTraceiQ3E840.
PROiQ3E840.

Family and domain databases

InterProiIPR007872. Znf_DHP.
[Graphical view]
PfamiPF05207. zf-CSL. 1 hit.
[Graphical view]
PROSITEiPS51074. ZF_DPH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPH3_YEAST
AccessioniPrimary (citable) accession number: Q3E840
Secondary accession number(s): D6VPT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.