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Protein

Diphthamide biosynthesis protein 3

Gene

KTI11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2. Required for conferring sensitivity to K.lactis zymocin.1 Publication

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5957DPH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • electron carrier activity Source: SGD
  • iron ion binding Source: SGD
  • zinc ion binding Source: SGD

GO - Biological processi

  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: SGD
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15580.
YEAST:MONOMER-15580.
ReactomeiR-SCE-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthamide biosynthesis protein 3
Alternative name(s):
Kluyveromyces lactis toxin-insensitive protein 11
Gene namesi
Name:KTI11
Synonyms:DPH3
Ordered Locus Names:YBL071W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL071W-A.
SGDiS000007587. KTI11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8282Diphthamide biosynthesis protein 3PRO_0000082639Add
BLAST

Proteomic databases

MaxQBiQ3E840.

Interactioni

Subunit structurei

Interacts with the diphthamide biosynthesis proteins DPH1 and DPH2. Interacts with elongation factor 2 (EFT2), the core-elongator subunits IKI3/ELP1, ELP2 and ELP3, and the 40S ribosomal proteins RPS7A and RPS19A.1 Publication

Protein-protein interaction databases

BioGridi32630. 21 interactions.
DIPiDIP-52225N.
IntActiQ3E840. 4 interactions.
MINTiMINT-2786415.

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Helixi9 – 113Combined sources
Beta strandi12 – 154Combined sources
Turni16 – 194Combined sources
Beta strandi20 – 245Combined sources
Beta strandi28 – 347Combined sources
Helixi35 – 395Combined sources
Beta strandi44 – 463Combined sources
Beta strandi48 – 514Combined sources
Beta strandi53 – 575Combined sources
Helixi60 – 7011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YOPNMR-A1-82[»]
1YWSNMR-A1-82[»]
4D4OX-ray2.90A/B/C1-82[»]
4D4PX-ray3.00A/B/C/E/G/H1-82[»]
4X33X-ray1.45A1-57[»]
ProteinModelPortaliQ3E840.
SMRiQ3E840. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3E840.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi79 – 824Poly-Ala

Sequence similaritiesi

Belongs to the DPH3 family.Curated
Contains 1 DPH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5957DPH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000007225.
HOGENOMiHOG000190740.
InParanoidiQ3E840.
KOiK15455.
OMAiREMLEMG.
OrthoDBiEOG7MSN28.

Family and domain databases

InterProiIPR007872. Znf_DHP.
[Graphical view]
PfamiPF05207. zf-CSL. 1 hit.
[Graphical view]
PROSITEiPS51074. ZF_DPH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3E840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTYDEIEIE DMTFEPENQM FTYPCPCGDR FQIYLDDMFE GEKVAVCPSC
60 70 80
SLMIDVVFDK EDLAEYYEEA GIHPPEPIAA AA
Length:82
Mass (Da):9,356
Last modified:November 8, 2005 - v1
Checksum:i8B27A307831B0ECE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35832 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07051.1.
RefSeqiNP_660100.1. NM_001184454.1.

Genome annotation databases

EnsemblFungiiYBL071W-A; YBL071W-A; YBL071W-A.
GeneIDi852207.
KEGGisce:YBL071W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35832 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07051.1.
RefSeqiNP_660100.1. NM_001184454.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YOPNMR-A1-82[»]
1YWSNMR-A1-82[»]
4D4OX-ray2.90A/B/C1-82[»]
4D4PX-ray3.00A/B/C/E/G/H1-82[»]
4X33X-ray1.45A1-57[»]
ProteinModelPortaliQ3E840.
SMRiQ3E840. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32630. 21 interactions.
DIPiDIP-52225N.
IntActiQ3E840. 4 interactions.
MINTiMINT-2786415.

Proteomic databases

MaxQBiQ3E840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL071W-A; YBL071W-A; YBL071W-A.
GeneIDi852207.
KEGGisce:YBL071W-A.

Organism-specific databases

EuPathDBiFungiDB:YBL071W-A.
SGDiS000007587. KTI11.

Phylogenomic databases

GeneTreeiENSGT00390000007225.
HOGENOMiHOG000190740.
InParanoidiQ3E840.
KOiK15455.
OMAiREMLEMG.
OrthoDBiEOG7MSN28.

Enzyme and pathway databases

UniPathwayiUPA00559.
BioCyciMetaCyc:MONOMER-15580.
YEAST:MONOMER-15580.
ReactomeiR-SCE-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

EvolutionaryTraceiQ3E840.
PROiQ3E840.

Family and domain databases

InterProiIPR007872. Znf_DHP.
[Graphical view]
PfamiPF05207. zf-CSL. 1 hit.
[Graphical view]
PROSITEiPS51074. ZF_DPH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Elongator's toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification."
    Fichtner L., Jablonowski D., Schierhorn A., Kitamoto H.K., Stark M.J.R., Schaffrath R.
    Mol. Microbiol. 49:1297-1307(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DPH1; DPH2; ELP1; ELP2; ELP3; EFT2; RPS7 AND RPS19.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2."
    Liu S., Milne G.T., Kuremsky J.G., Fink G.R., Leppla S.H.
    Mol. Cell. Biol. 24:9487-9497(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module."
    Sun J., Zhang J., Wu F., Xu C., Li S., Zhao W., Wu Z., Wu J., Zhou C.-Z., Shi Y.
    Biochemistry 44:8801-8809(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, ZINC-BINDING.

Entry informationi

Entry nameiDPH3_YEAST
AccessioniPrimary (citable) accession number: Q3E840
Secondary accession number(s): D6VPT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 8, 2005
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.