Q3E834 (YOS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein transport protein YOS1

Alternative name(s):
YIP one suppressor protein 1

Gene names

Name:	YOS1
Ordered Locus Names:	YER074W-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein transport between endoplasmic reticulum and Golgi apparatus. Ref.3
Subunit structure	Component of the YIP1-YIF1 complex, composed of at least YIF1, YIP1 and YOS1.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Ref.3.
Sequence similarities	Belongs to the YOS1 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.3. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER to Golgi transport vesicle Inferred from direct assay Ref.3. Source: SGD endoplasmic reticulum membrane Inferred from direct assay Ref.3. Source: SGD integral to Golgi membrane Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 85	85	Protein transport protein YOS1		PRO_0000232636
Regions
	Transmembrane	2 – 22	21	Helical; Potential
	Transmembrane	65 – 85	21	Helical; Potential
Experimental info
	Mutagenesis	18	1	N → A in YOS1-1; impairs interaction with YIP1, blocks ER-Golgi protein transport, and causes a severe growth defect at 37 degrees Celsius. Ref.3
	Mutagenesis	69	1	P → A: Lethal. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q3E834 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 7AE4E85AD2B43787	FASTA	85	9,436
	10 20 30 40 50 60 MVLFGLGRLF YVILLLINAV AVLSEERFLR RIGLGRSNDE TPVFGQDQNT TKSKVVQLIG 70 80 AVQTLLRIPL IGINILVIVY ELLLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the Golgi complex." Heidtman M., Chen C.Z., Collins R.N., Barlowe C. Mol. Biol. Cell 16:1673-1683(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH YIF1 AND YIP1, SUBCELLULAR LOCATION, MUTAGENESIS OF ASN-18 AND PRO-69.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18814 Genomic DNA. No translation available. BK006939 Genomic DNA. Translation: DAA07734.1.
RefSeq	NP_219496.2. NM_001184512.1.
3D structure databases
ProteinModelPortal	Q3E834.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YER074W-A.
Proteomic databases
PaxDb	Q3E834.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER074W-A; YER074W-A; YER074W-A.
GeneID	856806.
KEGG	sce:YER074W-A.
Organism-specific databases
CYGD	YER074w-a.
SGD	S000007651. YOS1.
Phylogenomic databases
eggNOG	NOG329574.
GeneTree	ENSGT00510000047648.
HOGENOM	HOG000192480.
OMA	FLNRIGW.
OrthoDB	EOG451J1J.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30392-MONOMER.
Gene expression databases
Genevestigator	Q3E834.
Family and domain databases
InterPro	IPR013880. Yos1. [Graphical view]
PANTHER	PTHR15858. PTHR15858. 1 hit.
Pfam	PF08571. Yos1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983062.

Entry information

Entry name

YOS1_YEAST

Accession

Primary (citable) accession number: Q3E834
Secondary accession number(s): D3DLY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	November 8, 2005
Last modified:	May 29, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program