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Protein

40S ribosomal protein S28-A

Gene

RPS28A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA Source: GO_Central
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD
  • ribosomal small subunit assembly Source: GO_Central
  • rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-33683-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S28-A
Alternative name(s):
S33
YS27
Gene namesi
Name:RPS28A
Synonyms:RPS33, RPS33A
Ordered Locus Names:YOR167C
ORF Names:O3600
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR167C.
SGDiS000005693. RPS28A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001368421 – 6740S ribosomal protein S28-AAdd BLAST67

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatB.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ3E7X9.
PRIDEiQ3E7X9.
TopDownProteomicsiQ3E7X9.

PTM databases

iPTMnetiQ3E7X9.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi34563. 39 interactors.
DIPiDIP-29384N.
IntActiQ3E7X9. 2 interactors.
MINTiMINT-390375.

Structurei

Secondary structure

167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 23Combined sources15
Beta strandi25 – 35Combined sources11
Beta strandi39 – 46Combined sources8
Beta strandi53 – 55Combined sources3
Beta strandi57 – 60Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10281-67[»]
3J6Yelectron microscopy6.10281-67[»]
3J77electron microscopy6.20281-67[»]
3J78electron microscopy6.30281-67[»]
3V88X-ray3.00c1-67[»]
4U3MX-ray3.00D8/d82-67[»]
4U3NX-ray3.20D8/d82-67[»]
4U3UX-ray2.90D8/d82-67[»]
4U4NX-ray3.10D8/d82-67[»]
4U4OX-ray3.60D8/d82-67[»]
4U4QX-ray3.00D8/d82-67[»]
4U4RX-ray2.80D8/d82-67[»]
4U4UX-ray3.00D8/d82-67[»]
4U4YX-ray3.20D8/d82-67[»]
4U4ZX-ray3.10D8/d82-67[»]
4U50X-ray3.20D8/d82-67[»]
4U51X-ray3.20D8/d82-67[»]
4U52X-ray3.00D8/d82-67[»]
4U53X-ray3.30D8/d82-67[»]
4U55X-ray3.20D8/d82-67[»]
4U56X-ray3.45D8/d82-67[»]
4U6FX-ray3.10D8/d82-67[»]
4V6Ielectron microscopy8.80AY1-67[»]
4V7RX-ray4.00AR/CR1-67[»]
4V88X-ray3.00Ac/Cc1-67[»]
4V8Yelectron microscopy4.30A21-67[»]
4V8Zelectron microscopy6.60A21-67[»]
4V92electron microscopy3.70c5-67[»]
5DATX-ray3.15D8/d82-67[»]
5DC3X-ray3.25D8/d82-67[»]
5FCIX-ray3.40D8/d82-67[»]
5FCJX-ray3.10D8/d82-67[»]
5I4LX-ray3.10D8/d85-67[»]
5JPQelectron microscopy7.3011-67[»]
5JUOelectron microscopy4.00ZB1-67[»]
5JUPelectron microscopy3.50ZB1-67[»]
5JUSelectron microscopy4.20ZB1-67[»]
5JUTelectron microscopy4.00ZB1-67[»]
5JUUelectron microscopy4.00ZB1-67[»]
ProteinModelPortaliQ3E7X9.
SMRiQ3E7X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S28e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003580.
HOGENOMiHOG000224552.
InParanoidiQ3E7X9.
KOiK02979.
OMAiPVRTNDI.
OrthoDBiEOG092C5Y3A.

Family and domain databases

CDDicd04457. S1_S28E. 1 hit.
HAMAPiMF_00292. Ribosomal_S28e. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR000289. Ribosomal_S28e.
IPR028626. Ribosomal_S28e_CS.
[Graphical view]
PANTHERiPTHR10769. PTHR10769. 1 hit.
PfamiPF01200. Ribosomal_S28e. 1 hit.
[Graphical view]
ProDomiPD005541. Ribosomal_S28e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00961. RIBOSOMAL_S28E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3E7X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE
60
NDILVLMESE REARRLR
Length:67
Mass (Da):7,592
Last modified:November 8, 2005 - v1
Checksum:iBA145824DEE8A641
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00128 Genomic DNA. Translation: CAA24958.1.
U55021 Genomic DNA. Translation: AAB47414.1.
Z75075 Genomic DNA. Translation: CAA99373.1.
AY692746 Genomic DNA. Translation: AAT92765.1.
BK006948 Genomic DNA. Translation: DAA10941.1.
PIRiA02750. R3BY33.
RefSeqiNP_014810.1. NM_001183586.1.

Genome annotation databases

EnsemblFungiiYOR167C; YOR167C; YOR167C.
GeneIDi854338.
KEGGisce:YOR167C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00128 Genomic DNA. Translation: CAA24958.1.
U55021 Genomic DNA. Translation: AAB47414.1.
Z75075 Genomic DNA. Translation: CAA99373.1.
AY692746 Genomic DNA. Translation: AAT92765.1.
BK006948 Genomic DNA. Translation: DAA10941.1.
PIRiA02750. R3BY33.
RefSeqiNP_014810.1. NM_001183586.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10281-67[»]
3J6Yelectron microscopy6.10281-67[»]
3J77electron microscopy6.20281-67[»]
3J78electron microscopy6.30281-67[»]
3V88X-ray3.00c1-67[»]
4U3MX-ray3.00D8/d82-67[»]
4U3NX-ray3.20D8/d82-67[»]
4U3UX-ray2.90D8/d82-67[»]
4U4NX-ray3.10D8/d82-67[»]
4U4OX-ray3.60D8/d82-67[»]
4U4QX-ray3.00D8/d82-67[»]
4U4RX-ray2.80D8/d82-67[»]
4U4UX-ray3.00D8/d82-67[»]
4U4YX-ray3.20D8/d82-67[»]
4U4ZX-ray3.10D8/d82-67[»]
4U50X-ray3.20D8/d82-67[»]
4U51X-ray3.20D8/d82-67[»]
4U52X-ray3.00D8/d82-67[»]
4U53X-ray3.30D8/d82-67[»]
4U55X-ray3.20D8/d82-67[»]
4U56X-ray3.45D8/d82-67[»]
4U6FX-ray3.10D8/d82-67[»]
4V6Ielectron microscopy8.80AY1-67[»]
4V7RX-ray4.00AR/CR1-67[»]
4V88X-ray3.00Ac/Cc1-67[»]
4V8Yelectron microscopy4.30A21-67[»]
4V8Zelectron microscopy6.60A21-67[»]
4V92electron microscopy3.70c5-67[»]
5DATX-ray3.15D8/d82-67[»]
5DC3X-ray3.25D8/d82-67[»]
5FCIX-ray3.40D8/d82-67[»]
5FCJX-ray3.10D8/d82-67[»]
5I4LX-ray3.10D8/d85-67[»]
5JPQelectron microscopy7.3011-67[»]
5JUOelectron microscopy4.00ZB1-67[»]
5JUPelectron microscopy3.50ZB1-67[»]
5JUSelectron microscopy4.20ZB1-67[»]
5JUTelectron microscopy4.00ZB1-67[»]
5JUUelectron microscopy4.00ZB1-67[»]
ProteinModelPortaliQ3E7X9.
SMRiQ3E7X9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34563. 39 interactors.
DIPiDIP-29384N.
IntActiQ3E7X9. 2 interactors.
MINTiMINT-390375.

PTM databases

iPTMnetiQ3E7X9.

Proteomic databases

MaxQBiQ3E7X9.
PRIDEiQ3E7X9.
TopDownProteomicsiQ3E7X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR167C; YOR167C; YOR167C.
GeneIDi854338.
KEGGisce:YOR167C.

Organism-specific databases

EuPathDBiFungiDB:YOR167C.
SGDiS000005693. RPS28A.

Phylogenomic databases

GeneTreeiENSGT00390000003580.
HOGENOMiHOG000224552.
InParanoidiQ3E7X9.
KOiK02979.
OMAiPVRTNDI.
OrthoDBiEOG092C5Y3A.

Enzyme and pathway databases

BioCyciYEAST:G3O-33683-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiQ3E7X9.

Family and domain databases

CDDicd04457. S1_S28E. 1 hit.
HAMAPiMF_00292. Ribosomal_S28e. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR000289. Ribosomal_S28e.
IPR028626. Ribosomal_S28e_CS.
[Graphical view]
PANTHERiPTHR10769. PTHR10769. 1 hit.
PfamiPF01200. Ribosomal_S28e. 1 hit.
[Graphical view]
ProDomiPD005541. Ribosomal_S28e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00961. RIBOSOMAL_S28E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS28A_YEAST
AccessioniPrimary (citable) accession number: Q3E7X9
Secondary accession number(s): D6W2M5, P02380
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 8, 2005
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 94500 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S28 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.