Q3E7X9 (RS28A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S28-A

Alternative name(s):
S33
YS27

Gene names

Name:	RPS28A
Synonyms:	RPS33, RPS33A
Ordered Locus Names:	YOR167C
ORF Names:	O3600

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	67 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.8.
Post-translational modification	N-terminally acetylated by acetyltransferase NatB. HAMAP-Rule MF_00292
Miscellaneous	Present with 94500 molecules/cell in log phase SD medium. HAMAP-Rule MF_00292 There are 2 genes for S28 in yeast. HAMAP-Rule MF_00292
Sequence similarities	Belongs to the ribosomal protein S28e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator PubMed 3533916. Source: SGD rRNA export from nucleus Inferred from genetic interaction PubMed 16246728. Source: SGD
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay PubMed 3533916. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay PubMed 3533916. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 67

67

40S ribosomal protein S28-A HAMAP-Rule MF_00292

PRO_0000136842

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.7

Modified residue

21

1

Phosphoserine Ref.10

Modified residue

26

1

Phosphothreonine Ref.10

Secondary structure

1

.

.

.

.

.

.

.

.

.

67

Helix Strand Turn

Details...

Beta strand

8 – 22

15

Beta strand

25 – 35

11

Beta strand

39 – 43

5

Beta strand

53 – 60

8

Sequences

	Sequence		Length	Mass (Da)
	Q3E7X9 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BA145824DEE8A641	FASTA	67	7,592
	10 20 30 40 50 60 MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast ribosomal protein S33 is encoded by an unsplit gene." Leer R.J., van Raamsdonk-Duin M.M.C., Schoppink P.J., Cornelissen M.T.E., Cohen L.H., Mager W.H., Planta R.J. Nucleic Acids Res. 11:7759-7768(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV." Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P. Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1678.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT MET-1 BY NATB.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21 AND THR-26, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X00128 Genomic DNA. Translation: CAA24958.1.
U55021 Genomic DNA. Translation: AAB47414.1.
Z75075 Genomic DNA. Translation: CAA99373.1.
AY692746 Genomic DNA. Translation: AAT92765.1.
BK006948 Genomic DNA. Translation: DAA10941.1.

PIR

R3BY33. A02750.

RefSeq

NP_014810.1. NM_001183586.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZB	electron microscopy	-	Y	1-67	[»]
3O2Z	X-ray	4.00	R	1-67	[»]
3O30	X-ray	4.00	R	1-67	[»]
3U5C	X-ray	3.00	c	1-67	[»]
3U5G	X-ray	3.00	c	1-67	[»]

ProteinModelPortal

Q3E7X9.

SMR

Q3E7X9. Positions 5-67.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29384N.

IntAct

Q3E7X9. 1 interaction.

MINT

MINT-390375.

Proteomic databases

PaxDb

Q3E7X9.

PRIDE

Q3E7X9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOR167C; YOR167C; YOR167C.

GeneID

854338.

KEGG

sce:YOR167C.

Organism-specific databases

CYGD

YOR167c.

SGD

S000005693. RPS28A.

Phylogenomic databases

eggNOG

COG2053.

GeneTree

ENSGT00390000003580.

HOGENOM

HOG000224552.

KO

K02979.

OMA

EFMEDTS.

OrthoDB

EOG4STWFH.

Gene expression databases

Genevestigator

Q3E7X9.

Family and domain databases

Gene3D

2.40.50.140. 1 hit.

HAMAP

MF_00292. Ribosomal_S28e.

InterPro

IPR012340. NA-bd_OB-fold.
IPR000289. Ribosomal_S28e.
[Graphical view]

PANTHER

PTHR10769. PTHR10769. 1 hit.

Pfam

PF01200. Ribosomal_S28e. 1 hit.
[Graphical view]

ProDom

PD005541. Ribosomal_S28e. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.

PROSITE

PS00961. RIBOSOMAL_S28E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

976408.

Entry information

Entry name

RS28A_YEAST

Accession

Primary (citable) accession number: Q3E7X9
Secondary accession number(s): D6W2M5, P02380

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 8, 2005
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program