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Q3E7X9 (RS28A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S28-A
Alternative name(s):
S33
YS27
Gene names
Name:RPS28A
Synonyms:RPS33, RPS33A
Ordered Locus Names:YOR167C
ORF Names:O3600
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length67 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6

Subcellular location

Cytoplasm Ref.8.

Post-translational modification

N-terminally acetylated by acetyltransferase NatB. Ref.7

Miscellaneous

Present with 94500 molecules/cell in log phase SD medium.

There are 2 genes for S28 in yeast.

Sequence similarities

Belongs to the ribosomal protein S28e family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 676740S ribosomal protein S28-A HAMAP-Rule MF_00292
PRO_0000136842

Amino acid modifications

Modified residue11N-acetylmethionine Ref.7 Ref.10

Secondary structure

......... 67
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q3E7X9 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: BA145824DEE8A641

FASTA677,592
        10         20         30         40         50         60 
MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE 


REARRLR 

« Hide

References

« Hide 'large scale' references
[1]"Yeast ribosomal protein S33 is encoded by an unsplit gene."
Leer R.J., van Raamsdonk-Duin M.M.C., Schoppink P.J., Cornelissen M.T.E., Cohen L.H., Mager W.H., Planta R.J.
Nucleic Acids Res. 11:7759-7768(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV."
Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
Yeast 12:1563-1573(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1678.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[7]"The action of N-terminal acetyltransferases on yeast ribosomal proteins."
Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT MET-1 BY NATB.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00128 Genomic DNA. Translation: CAA24958.1.
U55021 Genomic DNA. Translation: AAB47414.1.
Z75075 Genomic DNA. Translation: CAA99373.1.
AY692746 Genomic DNA. Translation: AAT92765.1.
BK006948 Genomic DNA. Translation: DAA10941.1.
PIRR3BY33. A02750.
RefSeqNP_014810.1. NM_001183586.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IZBelectron microscopy-Y1-67[»]
3O2ZX-ray4.00R1-67[»]
3O30X-ray4.00R1-67[»]
3U5CX-ray3.00c1-67[»]
3U5GX-ray3.00c1-67[»]
4BYLelectron microscopy4.3021-67[»]
4BYTelectron microscopy6.6021-67[»]
ProteinModelPortalQ3E7X9.
SMRQ3E7X9. Positions 5-67.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34563. 39 interactions.
DIPDIP-29384N.
IntActQ3E7X9. 1 interaction.
MINTMINT-390375.

Proteomic databases

PaxDbQ3E7X9.
PRIDEQ3E7X9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR167C; YOR167C; YOR167C.
GeneID854338.
KEGGsce:YOR167C.

Organism-specific databases

CYGDYOR167c.
SGDS000005693. RPS28A.

Phylogenomic databases

eggNOGCOG2053.
GeneTreeENSGT00390000003580.
HOGENOMHOG000224552.
KOK02979.
OMAPVRTNDI.
OrthoDBEOG7NCVH3.

Enzyme and pathway databases

BioCycYEAST:G3O-33683-MONOMER.

Gene expression databases

GenevestigatorQ3E7X9.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00292. Ribosomal_S28e.
InterProIPR012340. NA-bd_OB-fold.
IPR000289. Ribosomal_S28e.
IPR028626. Ribosomal_S28e_CS.
[Graphical view]
PANTHERPTHR10769. PTHR10769. 1 hit.
PfamPF01200. Ribosomal_S28e. 1 hit.
[Graphical view]
ProDomPD005541. Ribosomal_S28e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF50249. SSF50249. 1 hit.
PROSITEPS00961. RIBOSOMAL_S28E. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976408.

Entry information

Entry nameRS28A_YEAST
AccessionPrimary (citable) accession number: Q3E7X9
Secondary accession number(s): D6W2M5, P02380
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 8, 2005
Last modified: March 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references