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Q3E7T8

- UBQ14_ARATH

UniProt

Q3E7T8 - UBQ14_ARATH

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Protein

Polyubiquitin 14

Gene

UBQ14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_247278. ISG15 antiviral mechanism.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyubiquitin 14
Cleaved into the following chain:
Gene namesi
Name:UBQ14
Ordered Locus Names:At4g02890
ORF Names:T5J8.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G02890.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676UbiquitinPRO_0000396899Add
BLAST
Chaini77 – 15276UbiquitinPRO_0000396900Add
BLAST
Chaini153 – 22876UbiquitinPRO_0000396901Add
BLAST
Chaini229 – 30476UbiquitinPRO_0000396902Add
BLAST
Propeptidei305 – 3051CuratedPRO_0000396903

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki11 – 11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki29 – 29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki33 – 33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki48 – 48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki63 – 63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiQ3E7T8.

Expressioni

Gene expression databases

ExpressionAtlasiQ3E7T8. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi11158. 1 interaction.
11191. 11 interactions.
13437. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ3E7T8.
SMRiQ3E7T8. Positions 1-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7676Ubiquitin-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini77 – 15276Ubiquitin-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini153 – 22876Ubiquitin-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini229 – 30476Ubiquitin-like 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin family.Curated
Contains 4 ubiquitin-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiQ3E7T8.
KOiK08770.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamiPF00240. ubiquitin. 4 hits.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 4 hits.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 4 hits.
PROSITEiPS00299. UBIQUITIN_1. 4 hits.
PS50053. UBIQUITIN_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3E7T8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLADYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVESSDTID
110 120 130 140 150
NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLADYNIQKE STLHLVLRLR
160 170 180 190 200
GGMQIFVKTL TGKTITLEVE SSDTIDNVKA KIQDKEGIPP DQQRLIFAGK
210 220 230 240 250
QLEDGRTLAD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVESSDT
260 270 280 290 300
IDNVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLADYNIQ KESTLHLVLR

LRGGF
Length:305
Mass (Da):34,192
Last modified:October 13, 2009 - v2
Checksum:i901C32F1D4F3BE8F
GO
Isoform 2 (identifier: Q3E7T8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:229
Mass (Da):25,685
Checksum:iDECF8BB1742D009E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7676Missing in isoform 2. CuratedVSP_041601Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05394 Genomic DNA. No translation available.
AC004044 Genomic DNA. Translation: AAD15340.1.
AL161495 Genomic DNA. Translation: CAB77774.1.
CP002687 Genomic DNA. Translation: AEE82244.1.
CP002687 Genomic DNA. Translation: AEE82245.1.
CP002687 Genomic DNA. Translation: AEE82246.1.
CP002687 Genomic DNA. Translation: AEE82247.1.
AK316842 mRNA. Translation: BAH19554.1.
AY087759 mRNA. Translation: AAM65295.1.
RefSeqiNP_001031585.1. NM_001036508.1. [Q3E7T8-2]
NP_001118922.1. NM_001125450.1. [Q3E7T8-1]
NP_001118936.1. NM_001125464.2. [Q3E7T8-2]
NP_567247.2. NM_116523.3. [Q3E7T8-2]
NP_567286.1. NM_116744.3. [Q3E7T8-2]
NP_849291.1. NM_178960.2. [Q3E7T8-2]
NP_849292.1. NM_178961.1. [Q3E7T8-1]
NP_974516.4. NM_202787.4. [Q3E7T8-1]
UniGeneiAt.23108.
At.24970.
At.25274.
At.47590.
At.48785.
At.74401.
At.74780.

Genome annotation databases

EnsemblPlantsiAT4G02890.3; AT4G02890.3; AT4G02890.
AT4G02890.4; AT4G02890.4; AT4G02890.
AT4G05320.1; AT4G05320.1; AT4G05320.
AT4G05320.2; AT4G05320.2; AT4G05320.
AT4G05320.3; AT4G05320.3; AT4G05320.
AT4G05320.4; AT4G05320.4; AT4G05320.
AT4G05320.5; AT4G05320.5; AT4G05320.
AT4G05320.6; AT4G05320.6; AT4G05320.
GeneIDi825847.
825880.
828148.
KEGGiath:AT4G02890.
ath:AT4G05050.
ath:AT4G05320.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05394 Genomic DNA. No translation available.
AC004044 Genomic DNA. Translation: AAD15340.1 .
AL161495 Genomic DNA. Translation: CAB77774.1 .
CP002687 Genomic DNA. Translation: AEE82244.1 .
CP002687 Genomic DNA. Translation: AEE82245.1 .
CP002687 Genomic DNA. Translation: AEE82246.1 .
CP002687 Genomic DNA. Translation: AEE82247.1 .
AK316842 mRNA. Translation: BAH19554.1 .
AY087759 mRNA. Translation: AAM65295.1 .
RefSeqi NP_001031585.1. NM_001036508.1. [Q3E7T8-2 ]
NP_001118922.1. NM_001125450.1. [Q3E7T8-1 ]
NP_001118936.1. NM_001125464.2. [Q3E7T8-2 ]
NP_567247.2. NM_116523.3. [Q3E7T8-2 ]
NP_567286.1. NM_116744.3. [Q3E7T8-2 ]
NP_849291.1. NM_178960.2. [Q3E7T8-2 ]
NP_849292.1. NM_178961.1. [Q3E7T8-1 ]
NP_974516.4. NM_202787.4. [Q3E7T8-1 ]
UniGenei At.23108.
At.24970.
At.25274.
At.47590.
At.48785.
At.74401.
At.74780.

3D structure databases

ProteinModelPortali Q3E7T8.
SMRi Q3E7T8. Positions 1-303.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 11158. 1 interaction.
11191. 11 interactions.
13437. 3 interactions.

Proteomic databases

PRIDEi Q3E7T8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G02890.3 ; AT4G02890.3 ; AT4G02890 .
AT4G02890.4 ; AT4G02890.4 ; AT4G02890 .
AT4G05320.1 ; AT4G05320.1 ; AT4G05320 .
AT4G05320.2 ; AT4G05320.2 ; AT4G05320 .
AT4G05320.3 ; AT4G05320.3 ; AT4G05320 .
AT4G05320.4 ; AT4G05320.4 ; AT4G05320 .
AT4G05320.5 ; AT4G05320.5 ; AT4G05320 .
AT4G05320.6 ; AT4G05320.6 ; AT4G05320 .
GeneIDi 825847.
825880.
828148.
KEGGi ath:AT4G02890.
ath:AT4G05050.
ath:AT4G05320.

Organism-specific databases

TAIRi AT4G02890.

Phylogenomic databases

InParanoidi Q3E7T8.
KOi K08770.

Enzyme and pathway databases

Reactomei REACT_247278. ISG15 antiviral mechanism.

Gene expression databases

ExpressionAtlasi Q3E7T8. baseline and differential.

Family and domain databases

InterProi IPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view ]
Pfami PF00240. ubiquitin. 4 hits.
[Graphical view ]
PRINTSi PR00348. UBIQUITIN.
SMARTi SM00213. UBQ. 4 hits.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 4 hits.
PROSITEi PS00299. UBIQUITIN_1. 4 hits.
PS50053. UBIQUITIN_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and evolution of genes encoding polyubiquitin and ubiquitin-like proteins in Arabidopsis thaliana ecotype Columbia."
    Callis J., Carpenter T., Sun C.W., Vierstra R.D.
    Genetics 139:921-939(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
    Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
    Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-11; LYS-29; LYS-33; LYS-48 AND LYS-63.
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiUBQ14_ARATH
AccessioniPrimary (citable) accession number: Q3E7T8
Secondary accession number(s): B9DFN7
, O80715, P59263, Q38875, Q9LDJ2, Q9LYW1, Q9M0W3, Q9M1P9, Q9S7X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 13, 2009
Last modified: November 26, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Ubiquitin is encoded by 16 different genes. Ubiquitin is generally synthesized as a polyubiquitin precursor with tandem head to tail repeats. Often, there is one to three additional amino acids after the last repeat, removed in the mature protein. Alternatively, ubiquitin extension protein is synthesized as a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27A) or to a ubiquitin-related protein (either RUB1 or RUB2). Following translation, extension protein is cleaved from ubiquitin.
For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3