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Protein

Polyubiquitin 14

Gene

UBQ14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity

Miscellaneous

Ubiquitin is encoded by 16 different genes. Ubiquitin is generally synthesized as a polyubiquitin precursor with tandem head to tail repeats. Often, there is one to three additional amino acids after the last repeat, removed in the mature protein. Alternatively, ubiquitin extension protein is synthesized as a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27A) or to a ubiquitin-related protein (either RUB1 or RUB2). Following translation, extension protein is cleaved from ubiquitin.
For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Polyubiquitin 14
Cleaved into the following chain:
Gene namesi
Name:UBQ14
Ordered Locus Names:At4g02890
ORF Names:T5J8.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G02890
TAIRilocus:2140200 AT4G02890

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003968991 – 76UbiquitinAdd BLAST76
ChainiPRO_000039690077 – 152UbiquitinAdd BLAST76
ChainiPRO_0000396901153 – 228UbiquitinAdd BLAST76
ChainiPRO_0000396902229 – 304UbiquitinAdd BLAST76
PropeptideiPRO_0000396903305Curated1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond

Proteomic databases

PaxDbiQ3E7T8
PRIDEiQ3E7T8

Expressioni

Gene expression databases

ExpressionAtlasiQ3E7T8 baseline and differential
GenevisibleiQ3E7T8 AT

Interactioni

Protein-protein interaction databases

BioGridi11158, 1 interactor
11191, 13 interactors
13437, 3 interactors
STRINGi3702.AT4G02890.3

Structurei

3D structure databases

ProteinModelPortaliQ3E7T8
SMRiQ3E7T8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST76
Domaini77 – 152Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST76
Domaini153 – 228Ubiquitin-like 3PROSITE-ProRule annotationAdd BLAST76
Domaini229 – 304Ubiquitin-like 4PROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001 Eukaryota
COG5272 LUCA
InParanoidiQ3E7T8
KOiK08770
OrthoDBiEOG093608U5

Family and domain databases

InterProiView protein in InterPro
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 4 hits
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM00213 UBQ, 4 hits
SUPFAMiSSF54236 SSF54236, 4 hits
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 4 hits
PS50053 UBIQUITIN_2, 4 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3E7T8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLADYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVESSDTID
110 120 130 140 150
NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLADYNIQKE STLHLVLRLR
160 170 180 190 200
GGMQIFVKTL TGKTITLEVE SSDTIDNVKA KIQDKEGIPP DQQRLIFAGK
210 220 230 240 250
QLEDGRTLAD YNIQKESTLH LVLRLRGGMQ IFVKTLTGKT ITLEVESSDT
260 270 280 290 300
IDNVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLADYNIQ KESTLHLVLR

LRGGF
Length:305
Mass (Da):34,192
Last modified:October 13, 2009 - v2
Checksum:i901C32F1D4F3BE8F
GO
Isoform 2 (identifier: Q3E7T8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:229
Mass (Da):25,685
Checksum:iDECF8BB1742D009E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0416011 – 76Missing in isoform 2. CuratedAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05394 Genomic DNA No translation available.
AC004044 Genomic DNA Translation: AAD15340.1
AL161495 Genomic DNA Translation: CAB77774.1
CP002687 Genomic DNA Translation: AEE82244.1
CP002687 Genomic DNA Translation: AEE82245.1
CP002687 Genomic DNA Translation: AEE82246.1
CP002687 Genomic DNA Translation: AEE82247.1
AK316842 mRNA Translation: BAH19554.1
AY087759 mRNA Translation: AAM65295.1
RefSeqiNP_001031585.1, NM_001036508.2 [Q3E7T8-2]
NP_001118922.1, NM_001125450.2 [Q3E7T8-1]
NP_001118936.1, NM_001125464.2 [Q3E7T8-2]
NP_001328998.1, NM_001340547.1 [Q3E7T8-1]
NP_567247.2, NM_116523.3 [Q3E7T8-2]
NP_567286.1, NM_116744.4 [Q3E7T8-2]
NP_849291.1, NM_178960.2 [Q3E7T8-2]
NP_849292.1, NM_178961.1 [Q3E7T8-1]
NP_974516.4, NM_202787.4 [Q3E7T8-1]
UniGeneiAt.23108
At.24970
At.25274
At.47590
At.48785
At.74401
At.74780

Genome annotation databases

EnsemblPlantsiAT4G02890.1; AT4G02890.1; AT4G02890 [Q3E7T8-2]
AT4G02890.2; AT4G02890.2; AT4G02890 [Q3E7T8-2]
AT4G02890.3; AT4G02890.3; AT4G02890
AT4G02890.4; AT4G02890.4; AT4G02890
AT4G05050.1; AT4G05050.1; AT4G05050 [Q3E7T8-2]
AT4G05050.2; AT4G05050.2; AT4G05050 [Q3E7T8-2]
AT4G05050.3; AT4G05050.3; AT4G05050 [Q3E7T8-2]
AT4G05320.5; AT4G05320.5; AT4G05320
AT4G05320.8; AT4G05320.8; AT4G05320
GeneIDi825847
825880
828148
GrameneiAT4G02890.1; AT4G02890.1; AT4G02890 [Q3E7T8-2]
AT4G02890.2; AT4G02890.2; AT4G02890 [Q3E7T8-2]
AT4G02890.3; AT4G02890.3; AT4G02890
AT4G02890.4; AT4G02890.4; AT4G02890
AT4G05050.1; AT4G05050.1; AT4G05050 [Q3E7T8-2]
AT4G05050.2; AT4G05050.2; AT4G05050 [Q3E7T8-2]
AT4G05050.3; AT4G05050.3; AT4G05050 [Q3E7T8-2]
AT4G05320.5; AT4G05320.5; AT4G05320
AT4G05320.8; AT4G05320.8; AT4G05320
KEGGiath:AT4G02890
ath:AT4G05050
ath:AT4G05320

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBQ14_ARATH
AccessioniPrimary (citable) accession number: Q3E7T8
Secondary accession number(s): B9DFN7
, O80715, P59263, Q38875, Q9LDJ2, Q9LYW1, Q9M0W3, Q9M1P9, Q9S7X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 13, 2009
Last modified: May 23, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health