Q3E7B6 (VA0E_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type proton ATPase subunit e
Short name=V-ATPase subunit e

Alternative name(s):
Low dye-binding protein 10
Vacuolar proton pump subunit e

Gene names

Name:	VMA9
Synonyms:	CWH36, LDB10
Ordered Locus Names:	YCL005W-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	73 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the integral membrane V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Ref.5
Subunit structure	V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e). Ref.6
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.6.
Sequence similarities	Belongs to the V-ATPase e1/e2 subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro cell growth Inferred from genetic interaction PubMed 17350184. Source: UniProtKB cell wall mannoprotein biosynthetic process Inferred from mutant phenotype PubMed 14734024. Source: SGD protein complex assembly Inferred from mutant phenotype PubMed 16569636. Source: SGD vacuolar acidification Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	fungal-type vacuole membrane Inferred from mutant phenotype Ref.6. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar proton-transporting V-type ATPase, V0 domain Inferred from mutant phenotype Ref.6. Source: SGD
Molecular_function	ATPase activity, coupled to transmembrane movement of ions Inferred from mutant phenotype Ref.6. Source: SGD hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 73	73	V-type proton ATPase subunit e		PRO_0000071737
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	36 – 56	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3E7B6 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: B1440E10D0D34818	FASTA	73	8,381
	10 20 30 40 50 60 MSSFYTVVGV FIVVSAMSVL FWIMAPKNNQ AVWRSTVILT LAMMFLMWAI TFLCQLHPLV 70 APRRSDLRPE FAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204511 / S288c / AB972.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"Genome-wide analysis of iron-dependent growth reveals a novel yeast gene required for vacuolar acidification." Davis-Kaplan S.R., McVey Ward D., Shiflett S.L., Kaplan J. J. Biol. Chem. 279:4322-4329(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The yeast vacuolar proton-translocating ATPase contains a subunit homologous to the Manduca sexta and bovine e subunits that is essential for function." Sambade M., Kane P.M. J. Biol. Chem. 279:17361-17365(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. No translation available. BK006937 Genomic DNA. Translation: DAA07474.1.
RefSeq	NP_958835.1. NM_001184518.1.
3D structure databases
ProteinModelPortal	Q3E7B6.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YCL005W-A.
Proteomic databases
PaxDb	Q3E7B6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCL005W-A; YCL005W-A; YCL005W-A.
GeneID	2732686.
KEGG	sce:YCL005W-A.
Organism-specific databases
CYGD	YCL005w-a.
SGD	S000028508. VMA9.
Phylogenomic databases
eggNOG	NOG86391.
HOGENOM	HOG000000895.
OMA	MWAITFL.
OrthoDB	EOG4KD9WW.
Gene expression databases
Genevestigator	Q3E7B6.
Family and domain databases
InterPro	IPR008389. ATPase_V0-cplx_e1/e2_su. [Graphical view]
Pfam	PF05493. ATP_synt_H. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983847.

Entry information

Entry name

VA0E_YEAST

Accession

Primary (citable) accession number: Q3E7B6
Secondary accession number(s): D6VR05

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 8, 2005
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program