Q3E7B2 (COA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase assembly protein 3, mitochondrial

Alternative name(s):
Cytochrome c oxidase protein 25
Required for respiratory growth protein 10

Gene names

Name:	COA3
Synonyms:	COX25, RRG10
Ordered Locus Names:	YJL062W-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for assembly of cytochrome c oxidase (complex IV). With COX14, regulates negatively COX1 translation and is involved in MSS51 association with newly synthesized COX1. Ref.6 Ref.8 Ref.9 Ref.10
Subunit structure	Component of 250-400 kDa complexes called cytochrome oxidase assembly intermediates or COA complexes composed at least COA3, COX14, COX5A, SHY1 and SSC1. Interacts with COX1 and MSS51. Ref.9 Ref.10
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein Ref.4 Ref.7.
Miscellaneous	Present with 2530 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the COA3 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial respiratory chain complex IV assembly Inferred from mutant phenotype Ref.9Ref.10. Source: SGD negative regulation of mitochondrial translation Inferred from mutant phenotype Ref.9Ref.10. Source: SGD
Cellular_component	integral to mitochondrial inner membrane Inferred from direct assay Ref.10. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 85	85	Cytochrome c oxidase assembly protein 3, mitochondrial		PRO_0000245412
Regions
	Topological domain	1 – 26	26	Mitochondrial matrix Ref.9 Ref.10
	Transmembrane	27 – 49	23	Helical; Potential
	Topological domain	50 – 85	36	Mitochondrial intermembrane Ref.9 Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q3E7B2 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 41403CD21F898C18	FASTA	85	9,882
	10 20 30 40 50 60 MVLNPSKYQD TRTWKMTPAM IRARKPFFKG NMLGLTLLLG VTGSVYYYTY HFLHKDNDFA 70 80 DVPIPPIDPQ ELEALKKEYE AKKKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Genomic exploration of the hemiascomycetous yeasts: 4. The genome of Saccharomyces cerevisiae revisited." Blandin G., Durrens P., Tekaia F., Aigle M., Bolotin-Fukuhara M., Bon E., Casaregola S., de Montigny J., Gaillardin C., Lepingle A., Llorente B., Malpertuy A., Neuveglise C., Ozier-Kalogeropoulos O., Perrin A., Potier S., Souciet J.-L., Talla E. , Toffano-Nioche C., Wesolowski-Louvel M., Marck C., Dujon B. FEBS Lett. 487:31-36(2000) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae." Kastenmayer J.P., Ni L., Chu A., Kitchen L.E., Au W.-C., Yang H., Carter C.D., Wheeler D., Davis R.W., Boeke J.D., Snyder M.A., Basrai M.A. Genome Res. 16:365-373(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae." Merz S., Westermann B. Genome Biol. 10:R95.1-R95.18(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondria." Mick D.U., Vukotic M., Piechura H., Meyer H.E., Warscheid B., Deckers M., Rehling P. J. Cell Biol. 191:141-154(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN COA COMPLEXES, MASS SPECTROMETRY, INTERACTION WITH SHY1; COX1 AND MSS51, SUCELLULAR LOCATION, TOPOLOGY, FUNCTION.
[10]	"Cox25 teams up with Mss51, Ssc1, and Cox14 to regulate mitochondrial cytochrome c oxidase subunit 1 expression and assembly in Saccharomyces cerevisiae." Fontanesi F., Clemente P., Barrientos A. J. Biol. Chem. 286:555-566(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN COA COMPLEXES, MASS SPECTROMETRY, INTERACTION WITH COX1, SUCELLULAR LOCATION, TOPOLOGY, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49338 Genomic DNA. No translation available. BK006943 Genomic DNA. Translation: DAA08735.1.
RefSeq	NP_076894.3. NM_001184477.3.
3D structure databases
ProteinModelPortal	Q3E7B2.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-2492439.
STRING	4932.YJL062W-A.
Proteomic databases
PaxDb	Q3E7B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL062W-A; YJL062W-A; YJL062W-A.
GeneID	853383.
KEGG	sce:YJL060W. sce:YJL062W-A.
Organism-specific databases
CYGD	YJL062w-a.
SGD	S000007611. YJL062W-A.
Phylogenomic databases
eggNOG	NOG81147.
KO	K14264.
OMA	MTPAMIR.
OrthoDB	EOG4G1QS8.
Gene expression databases
Genevestigator	Q3E7B2.
Family and domain databases
ProtoNet	Search...
Other
NextBio	973840.

Entry information

Entry name

COA3_YEAST

Accession

Primary (citable) accession number: Q3E7B2
Secondary accession number(s): D6VWB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program