Q3E7A4 (COXM2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
COX assembly mitochondrial protein 2

Alternative name(s):
Cx9C motif-containing protein 2
Mitochondrial metallochaperone-like protein CMC2

Gene names

Name:	CMC2
Ordered Locus Names:	YBL059C-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration. May be involved in copper trafficking and distribution to mitochondrial COX and SOD1. Ref.5 Ref.6
Subunit structure	Interacts with CMC1. Ref.6
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note: Imported into the mitochondria via the mitochondrial MIA40-ERV1 machinery. Ref.5 Ref.6
Domain	The twin Cx9C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system and the subsequent transfer of disulfide bonds by dithiol/disulfide exchange reactions to the newly imported protein By similarity.
Miscellaneous	Present with 279 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CMC family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat
Ligand	Copper Metal-binding
Molecular function	Chaperone
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial respiratory chain complex assembly Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial intermembrane space Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SLU7	Q02775	1	EBI-2088942,EBI-17379

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 109	109	COX assembly mitochondrial protein 2		PRO_0000248414
Regions
	Motif	13 – 23	11	Cx9C motif 1
	Motif	36 – 46	11	Cx9C motif 2
Amino acid modifications
	Disulfide bond	13 ↔ 46		By similarity
	Disulfide bond	23 ↔ 36		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3E7A4 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 5D740EFEC60E5E5F	FASTA	109	12,947
	10 20 30 40 50 60 MHPQLEAERF HSCLDFINAL DKCHQKEYYK RIFGLCNNEK DALNKCLKEA SLNNKKRAVI 70 80 90 100 ESRIKRADVE KRWKKIEEEE YGEDAILKTI LDRQYAKKKQ ESDNDANSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / YPH1.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Systematic analysis of the twin cx(9)c protein family." Longen S., Bien M., Bihlmaier K., Kloeppel C., Kauff F., Hammermeister M., Westermann B., Herrmann J.M., Riemer J. J. Mol. Biol. 393:356-368(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"The conserved mitochondrial twin Cx9C protein Cmc2 Is a Cmc1 homologue essential for cytochrome c oxidase biogenesis." Horn D., Zhou W., Trevisson E., Al-Ali H., Harris T.K., Salviati L., Barrientos A. J. Biol. Chem. 285:15088-15099(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CMC1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23261 Genomic DNA. No translation available. Z35821 Genomic DNA. No translation available. BK006936 Genomic DNA. Translation: DAA07060.1.
RefSeq	NP_031358.1. NM_001184440.1.
3D structure databases
ProteinModelPortal	Q3E7A4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3E7A4. 1 interaction.
MINT	MINT-4476311.
STRING	4932.YBL059C-A.
Proteomic databases
PaxDb	Q3E7A4.
PeptideAtlas	Q3E7A4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL059C-A; YBL059C-A; YBL059C-A.
GeneID	852220.
KEGG	sce:YBL059C-A.
Organism-specific databases
CYGD	YBL059c-a.
SGD	S000007488. CMC2.
Phylogenomic databases
eggNOG	NOG125587.
OMA	CEKLMDA.
OrthoDB	EOG4RFQ39.
Gene expression databases
Genevestigator	Q3E7A4.
Family and domain databases
InterPro	IPR013892. Cyt_c_biogenesis_Cmc1-like. [Graphical view]
Pfam	PF08583. Cmc1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970736.

Entry information

Entry name

COXM2_YEAST

Accession

Primary (citable) accession number: Q3E7A4
Secondary accession number(s): D6VPU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 8, 2005
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program