Q3E7A4 (CMC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cx9C motif-Containing protein 2

Alternative name(s):
Mitochondrial metallochaperone-like protein CMC2

Gene names

Name:	CMC2
Ordered Locus Names:	YBL059C-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration. May be involved in copper trafficking and distribution to mitochondrial COX and SOD1. Ref.5 Ref.6
Subunit structure	Interacts with CMC1. Ref.6
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note: Imported in the mitochondrion inner membrane space by the MIA40-ERV1 machinery. Ref.5 Ref.6
Miscellaneous	Present with 279 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the CMC1 family. Contains 1 CHCH domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	Copper Metal-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial respiratory chain complex assembly Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial intermembrane space Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SLU7	Q02775	1	EBI-2088942,EBI-17379

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 109	109	Cx9C motif-Containing protein 2		PRO_0000248414
Regions
	Domain	6 – 49	44	CHCH

Sequences

	Sequence		Length	Mass (Da)
	Q3E7A4 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 5D740EFEC60E5E5F	FASTA	109	12,947
	10 20 30 40 50 60 MHPQLEAERF HSCLDFINAL DKCHQKEYYK RIFGLCNNEK DALNKCLKEA SLNNKKRAVI 70 80 90 100 ESRIKRADVE KRWKKIEEEE YGEDAILKTI LDRQYAKKKQ ESDNDANSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / YPH1.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Systematic analysis of the twin cx(9)c protein family." Longen S., Bien M., Bihlmaier K., Kloeppel C., Kauff F., Hammermeister M., Westermann B., Herrmann J.M., Riemer J. J. Mol. Biol. 393:356-368(2009) [PubMed: 19703468] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"The conserved mitochondrial twin Cx9C protein Cmc2 Is a Cmc1 homologue essential for cytochrome c oxidase biogenesis." Horn D., Zhou W., Trevisson E., Al-Ali H., Harris T.K., Salviati L., Barrientos A. J. Biol. Chem. 285:15088-15099(2010) [PubMed: 20220131] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CMC1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23261 Genomic DNA. No translation available. Z35821 Genomic DNA. No translation available. BK006936 Genomic DNA. Translation: DAA07060.1.
RefSeq	NP_031358.1. NM_001184440.1.
3D structure databases
ProteinModelPortal	Q3E7A4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3E7A4. 1 interaction.
MINT	MINT-4476311.
STRING	Q3E7A4.
Proteomic databases
PeptideAtlas	Q3E7A4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL059C-A; YBL059C-A; YBL059C-A.
GeneID	852220.
KEGG	sce:YBL059C-A.
NMPDR	fig|4932.3.peg.183.
Organism-specific databases
CYGD	YBL059c-a.
SGD	S000007488. CMC2.
Phylogenomic databases
eggNOG	fuNOG11703.
GeneTree	EFGT00050000006668.
HOGENOM	HBG397763.
OMA	ALEECHR.
OrthoDB	EOG4RFQ39.
Gene expression databases
ArrayExpress	Q3E7A4.
Genevestigator	Q3E7A4.
Family and domain databases
InterPro	IPR013892. Cyt_c_biogenesis_Cmc1-like. [Graphical view]
Pfam	PF08583. Cmc1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970736.

Entry information

Entry name

CMC2_YEAST

Accession

Primary (citable) accession number: Q3E7A4
Secondary accession number(s): D6VPU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 8, 2005
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program