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Protein

BolA-like protein 1

Gene

BOL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates [4Fe-4S] cluster insertion into a subset of mitochondrial proteins such as LIAS and succinate dehydrogenase (SDH) (PubMed:27532772). Required during the last step of iron-sulfur protein assembly when the iron-sulfur cluster is inserted into the target protein (PubMed:27532772). Probably acts together with the monothiol glutaredoxin GRX5, earlier than BOL3 and NFU1 in the [4Fe-4S] cluster insertion process (PubMed:27532773). Not required for [2Fe-2S] cluster insertion into mitochondrial proteins (PubMed:27532772).2 Publications

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • iron-sulfur cluster assembly Source: SGD
  • protein maturation by iron-sulfur cluster transfer Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-28901-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
BolA-like protein 1Curated
Gene namesi
Name:BOL12 Publications
Ordered Locus Names:YAL044W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL044W-A.
SGDiS000007586. BOL1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No visible phenotype (PubMed:27532773, PubMed:27532772). Cells lacking BOL1 and BOL3 display defects in a subset of mitochondrial [4Fe-4S] enzymes (PubMed:27532772).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56H → A: No effect. 1 Publication1
Mutagenesisi56H → C: Partial loss of function. 1 Publication1
Mutagenesisi93H → A or C: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002484091 – 110BolA-like protein 1Add BLAST110

Proteomic databases

MaxQBiQ3E793.
PRIDEiQ3E793.

Interactioni

Subunit structurei

Interacts with GRX5.1 Publication

Protein-protein interaction databases

BioGridi31782. 25 interactors.
IntActiQ3E793. 4 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ3E793.
SMRiQ3E793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BolA/IbaG family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000048165.
HOGENOMiHOG000255169.
InParanoidiQ3E793.
OMAiDSYKHAG.
OrthoDBiEOG092C5RHO.

Family and domain databases

InterProiView protein in InterPro
IPR002634. BolA.
PANTHERiPTHR12735. PTHR12735. 1 hit.
PfamiView protein in Pfam
PF01722. BolA. 1 hit.
PIRSFiPIRSF003113. BolA. 1 hit.
SUPFAMiSSF82657. SSF82657. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3E793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRAMSTDG PVARTILKRL ECGFPDYKNF AFGLYNDSHK HKGHAGVQGN
60 70 80 90 100
VSAETHFRIE MVSKKFEGLK LPQRHRMVYS LLQDEMAQAN GIHALQLSLK
110
TPQEYESKAK
Length:110
Mass (Da):12,537
Last modified:November 8, 2005 - v1
Checksum:iBF19118A3F4FDA35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. No translation available.
BK006935 Genomic DNA. Translation: DAA06941.1.
RefSeqiNP_075206.1. NM_001184453.1.

Genome annotation databases

EnsemblFungiiYAL044W-A; YAL044W-A; YAL044W-A.
GeneIDi851252.
KEGGisce:YAL044W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. No translation available.
BK006935 Genomic DNA. Translation: DAA06941.1.
RefSeqiNP_075206.1. NM_001184453.1.

3D structure databases

ProteinModelPortaliQ3E793.
SMRiQ3E793.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31782. 25 interactors.
IntActiQ3E793. 4 interactors.

Proteomic databases

MaxQBiQ3E793.
PRIDEiQ3E793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL044W-A; YAL044W-A; YAL044W-A.
GeneIDi851252.
KEGGisce:YAL044W-A.

Organism-specific databases

EuPathDBiFungiDB:YAL044W-A.
SGDiS000007586. BOL1.

Phylogenomic databases

GeneTreeiENSGT00510000048165.
HOGENOMiHOG000255169.
InParanoidiQ3E793.
OMAiDSYKHAG.
OrthoDBiEOG092C5RHO.

Enzyme and pathway databases

BioCyciYEAST:G3O-28901-MONOMER.

Miscellaneous databases

PROiPR:Q3E793.

Family and domain databases

InterProiView protein in InterPro
IPR002634. BolA.
PANTHERiPTHR12735. PTHR12735. 1 hit.
PfamiView protein in Pfam
PF01722. BolA. 1 hit.
PIRSFiPIRSF003113. BolA. 1 hit.
SUPFAMiSSF82657. SSF82657. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBOL1_YEAST
AccessioniPrimary (citable) accession number: Q3E793
Secondary accession number(s): D6VPH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 8, 2005
Last modified: June 7, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.