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U.S.A.10719748-19753STRUCTURE BY ELECTRON MICROSCOPY (8.80 ANGSTROMS)Crystal structure of the eukaryotic ribosome.Ben-Shem A.Jenner L.Yusupova G.Yusupov M.doi:10.1126/science.11942942010Science3301203-1209X-RAY CRYSTALLOGRAPHY (4.00 ANGSTROMS) OF 80S RIBOSOMEThe structure of the eukaryotic ribosome at 3.0 A resolution.Ben-Shem A.Garreau de Loubresse N.Melnikov S.Jenner L.Yusupova G.Yusupov M.doi:10.1126/science.12126422011Science3341524-1529X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 80S RIBOSOMESUBUNITSUBCELLULAR LOCATIONR3BY316.1025=1-1086.1025=1-1086.2025=1-1086.3025=1-1083.00D5/d5=2-1083.20D5/d5=2-1082.90D5/d5=2-1083.10D5/d5=2-1083.60D5/d5=2-1083.00D5/d5=2-1082.80D5/d5=2-1083.00D5/d5=2-1083.20D5/d5=2-1083.10D5/d5=2-1083.20D5/d5=2-1083.20D5/d5=2-1083.00D5/d5=2-1083.30D5/d5=2-1083.20D5/d5=2-1083.45D5/d5=2-1083.10D5/d5=2-1088.80AV=1-1084.00AQ/CQ=1-1083.00AZ/CZ=1-1084.30AZ=1-1086.60AZ=1-1083.70Z=42-1053.15D5/d5=2-1083.25D5/d5=2-1083.45D5/d5=2-1083.30D5/d5=2-1083.10D5/d5=2-1083.40D5/d5=2-1083.10D5/d5=2-1083.10D5/d5=36-1054.00WB=1-1083.50WB=1-1084.20WB=1-1084.00WB=1-1084.00WB=1-1083.25D5/d5=36-1053.30Z2=36-1053.80K=1-1083.50a/d5=36-1053.70D5/d5=36-1053.30D5/d5=36-1053.70D5/d5=36-1053.40D5/d5=36-1053.10a/d5=36-1053.00a/d5=36-1053.30D5/d5=36-1053.50D5/d5=36-1053.60K=1-1083.40Z=1-1084.40AP=36-1053.90AP=36-1054.00AP=36-1053.10a/d5=1-1085.30K/Kb=37-1053.10K=36-1053.47Z=1-1083.80Z=1-1083.28Ba=2-1082.80Z=1-1053.30K/Kb/Kc=1-1082.60SZ=24-1053.20SZ=1-1083.10SZ=1-1084.000/Zb=1-1082.80K=24-1053.10K=24-1053.70Z=42-1042.90ZZ=36-1053.80Z=1-1083.40SZ=1-1083.40SZ=1-1085.30a=1-1084.90DZ=1-1083.50DZ=1-1086.20M=1-1083.00J=37-1053.00L=24-1053.30K=24-1051.05D=2-73.05BZ=37-1052.70BZ=37-1053.05BZ=37-1053.90SK=24-1054.36SK=37-1053.47AZ=24-1054.80AZ=24-1054.30AZ=24-1052.50DZ=24-1052.40sK=1-1083.90K=24-1053.80K=24-1052.90K=1-1082.70K=1-1083.00L=1-1083.30M=1-1083.80Z=1-1081.971=1-1082.721=1-1082.041=1-1082.051=1-1082.711=1-1082.571=1-1082.281=1-1082.471=1-1083.111=1-1083.791=1-10836211RPS25AEukaryotaL13a-mediated translational silencing of Ceruloplasmin expressionSRP-dependent cotranslational protein targeting to membraneMajor pathway of rRNA processing in the nucleolus and cytosolTranslation initiation complex formationFormation of a pool of free 40S subunitsFormation of the ternary complex, and subsequently, the 43S complexRibosomal scanning and start codon recognitionGTP hydrolysis and joining of the 60S ribosomal subunitNonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)8 hits in 10 CRISPR screensProteinRibosomal_eS25WH_DNA-bd_sf40S RIBOSOMAL PROTEIN S2540S RIBOSOMAL PROTEIN S25Ribosomal_S25Winged helix' DNA-binding domainAA sequenceSmall ribosomal subunit protein eS25A40S ribosomal protein S25-ARP45S31YS23RPS25ARPS31AYGR027CComponent of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation.Present with 322000 molecules/cell in log phase SD medium.There are 2 genes for eS25 in yeast.Belongs to the eukaryotic ribosomal protein eS25 family.Removed1Small ribosomal subunit protein eS25A119082108Disordered30N,N-dimethylproline; by NTM1In strain: SK1.F8In strain: SK1.T13R164453545658606268697173858894991022005-11-081120399db1f09d860e4597977c95ab9586c433MPPKQQLSKAAKAAAALAGGKKSKKKWSKKSMKDRAQHAVILDQEKYDRILKEVPTYRYVSVSVLVDRLKIGGSLARIALRHLEKEGIIKPISKHSKQAIYTRATASEtruetruetruetruetruetruetruetruetruetruetruetruetruetrue