Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q3E792

- RS25A_YEAST

UniProt

Q3E792 - RS25A_YEAST

Protein

40S ribosomal protein S25-A

Gene

RPS25A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30751-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S25-A
    Alternative name(s):
    RP45
    S31
    YS23
    Gene namesi
    Name:RPS25A
    Synonyms:RPS31A
    Ordered Locus Names:YGR027C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR027c.
    SGDiS000003259. RPS25A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 10810740S ribosomal protein S25-APRO_0000043376Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N,N-dimethylproline; by NTM11 Publication

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiQ3E792.
    PaxDbiQ3E792.

    Expressioni

    Gene expression databases

    GenevestigatoriQ3E792.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-16106,EBI-16106

    Protein-protein interaction databases

    BioGridi33268. 81 interactions.
    IntActiQ3E792. 8 interactions.
    MINTiMINT-490524.

    Structurei

    Secondary structure

    1
    108
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi45 – 539
    Beta strandi58 – 614
    Helixi62 – 676
    Helixi75 – 839
    Beta strandi84 – 885
    Beta strandi90 – 934
    Beta strandi100 – 1023

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW9electron microscopy6.10a1-108[»]
    1VWVelectron microscopy6.10a1-108[»]
    3IZBelectron microscopy-V1-108[»]
    3O2ZX-ray4.00Q1-108[»]
    3O30X-ray4.00Q1-108[»]
    3U5CX-ray3.00Z1-108[»]
    3U5GX-ray3.00Z1-108[»]
    4BYLelectron microscopy4.30Z1-108[»]
    4BYTelectron microscopy6.60Z1-108[»]
    4CUYelectron microscopy3.70Z42-105[»]
    ProteinModelPortaliQ3E792.
    SMRiQ3E792. Positions 36-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S25e family.Curated

    Phylogenomic databases

    eggNOGiCOG4901.
    GeneTreeiENSGT00390000004856.
    HOGENOMiHOG000203673.
    KOiK02975.
    OMAiQLIYTRS.
    OrthoDBiEOG7647G6.

    Family and domain databases

    InterProiIPR004977. Ribosomal_S25.
    [Graphical view]
    PANTHERiPTHR12850. PTHR12850. 1 hit.
    PfamiPF03297. Ribosomal_S25. 1 hit.
    [Graphical view]
    ProDomiPD012268. Ribosomal_S25. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3E792-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI    50
    LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI 100
    YTRATASE 108
    Length:108
    Mass (Da):12,039
    Last modified:November 8, 2005 - v1
    Checksum:iC52D0328DEA125B5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161A → R AA sequence (PubMed:18782943)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti8 – 81S → F in strain: SK1. 1 Publication
    Natural varianti13 – 131A → T in strain: SK1. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03013 Genomic DNA. Translation: CAA26797.1.
    Z72812 Genomic DNA. Translation: CAA97010.1.
    DQ115389 Genomic DNA. Translation: AAZ22490.1.
    EF123137 mRNA. Translation: ABM97481.1.
    BK006941 Genomic DNA. Translation: DAA08121.1.
    PIRiS05844. R3BY31.
    RefSeqiNP_011541.1. NM_001181156.1.

    Genome annotation databases

    EnsemblFungiiYGR027C; YGR027C; YGR027C.
    GeneIDi852911.
    KEGGisce:YGR027C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03013 Genomic DNA. Translation: CAA26797.1 .
    Z72812 Genomic DNA. Translation: CAA97010.1 .
    DQ115389 Genomic DNA. Translation: AAZ22490.1 .
    EF123137 mRNA. Translation: ABM97481.1 .
    BK006941 Genomic DNA. Translation: DAA08121.1 .
    PIRi S05844. R3BY31.
    RefSeqi NP_011541.1. NM_001181156.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW9 electron microscopy 6.10 a 1-108 [» ]
    1VWV electron microscopy 6.10 a 1-108 [» ]
    3IZB electron microscopy - V 1-108 [» ]
    3O2Z X-ray 4.00 Q 1-108 [» ]
    3O30 X-ray 4.00 Q 1-108 [» ]
    3U5C X-ray 3.00 Z 1-108 [» ]
    3U5G X-ray 3.00 Z 1-108 [» ]
    4BYL electron microscopy 4.30 Z 1-108 [» ]
    4BYT electron microscopy 6.60 Z 1-108 [» ]
    4CUY electron microscopy 3.70 Z 42-105 [» ]
    ProteinModelPortali Q3E792.
    SMRi Q3E792. Positions 36-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33268. 81 interactions.
    IntActi Q3E792. 8 interactions.
    MINTi MINT-490524.

    Proteomic databases

    MaxQBi Q3E792.
    PaxDbi Q3E792.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR027C ; YGR027C ; YGR027C .
    GeneIDi 852911.
    KEGGi sce:YGR027C.

    Organism-specific databases

    CYGDi YGR027c.
    SGDi S000003259. RPS25A.

    Phylogenomic databases

    eggNOGi COG4901.
    GeneTreei ENSGT00390000004856.
    HOGENOMi HOG000203673.
    KOi K02975.
    OMAi QLIYTRS.
    OrthoDBi EOG7647G6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30751-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 972603.

    Gene expression databases

    Genevestigatori Q3E792.

    Family and domain databases

    InterProi IPR004977. Ribosomal_S25.
    [Graphical view ]
    PANTHERi PTHR12850. PTHR12850. 1 hit.
    Pfami PF03297. Ribosomal_S25. 1 hit.
    [Graphical view ]
    ProDomi PD012268. Ribosomal_S25. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene for yeast ribosomal protein S31 contains an intron in the leader sequence."
      Nieuwint R.T.M., Molenaar C.M.T., van Bommel J.H., van Raamsdonk-Duin M.M.C., Mager W.H., Planta R.J.
      Curr. Genet. 10:1-5(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Carlsbergensis.
    2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
      Juneau K., Palm C., Miranda M., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-96.
      Strain: ATCC 201390 / BY4743.
    6. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
      Deutschbauer A.M., Davis R.W.
      Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-108, VARIANTS PHE-8 AND THR-13.
      Strain: SK1.
    7. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
      Otaka E., Higo K., Itoh T.
      Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE OF 15-22.
    8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Identification of protein N-terminal methyltransferases in yeast and humans."
      Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
      Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT PRO-2 BY NTM1/TAE1.
    12. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.80 ANGSTROMS).
    13. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.00 ANGSTROMS).
    14. Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).

    Entry informationi

    Entry nameiRS25A_YEAST
    AccessioniPrimary (citable) accession number: Q3E792
    Secondary accession number(s): A2TBN4
    , D6VUG0, P05758, P07282, Q45U56
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation.
    Present with 322000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S25 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3