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Protein

40S ribosomal protein S25-A

Gene

RPS25A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation.1 Publication
Present with 322000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS25 in yeast.Curated

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30751-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S25-A1 Publication
Alternative name(s):
RP45
S31
Small ribosomal subunit protein eS25-A1 Publication
YS23
Gene namesi
Name:RPS25A1 Publication
Synonyms:RPS31A
Ordered Locus Names:YGR027C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR027C.
SGDiS000003259. RPS25A.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000433762 – 10840S ribosomal protein S25-AAdd BLAST107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N-dimethylproline; by NTM11 Publication1

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiQ3E792.
PRIDEiQ3E792.
TopDownProteomicsiQ3E792.

PTM databases

iPTMnetiQ3E792.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi33268. 267 interactors.
IntActiQ3E792. 13 interactors.
MINTiMINT-490524.
STRINGi4932.YGR027C.

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 41Combined sources3
Helixi45 – 53Combined sources9
Helixi54 – 56Combined sources3
Beta strandi58 – 60Combined sources3
Helixi62 – 67Combined sources6
Helixi73 – 83Combined sources11
Beta strandi84 – 91Combined sources8
Beta strandi95 – 97Combined sources3
Beta strandi101 – 103Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10251-108[»]
3J6Yelectron microscopy6.10251-108[»]
3J77electron microscopy6.20251-108[»]
3J78electron microscopy6.30251-108[»]
3V88X-ray3.00Z1-108[»]
4U3MX-ray3.00D5/d52-108[»]
4U3NX-ray3.20D5/d52-108[»]
4U3UX-ray2.90D5/d52-108[»]
4U4NX-ray3.10D5/d52-108[»]
4U4OX-ray3.60D5/d52-108[»]
4U4QX-ray3.00D5/d52-108[»]
4U4RX-ray2.80D5/d52-108[»]
4U4UX-ray3.00D5/d52-108[»]
4U4YX-ray3.20D5/d52-108[»]
4U4ZX-ray3.10D5/d52-108[»]
4U50X-ray3.20D5/d52-108[»]
4U51X-ray3.20D5/d52-108[»]
4U52X-ray3.00D5/d52-108[»]
4U53X-ray3.30D5/d52-108[»]
4U55X-ray3.20D5/d52-108[»]
4U56X-ray3.45D5/d52-108[»]
4U6FX-ray3.10D5/d52-108[»]
4V6Ielectron microscopy8.80AV1-108[»]
4V7RX-ray4.00AQ/CQ1-108[»]
4V88X-ray3.00AZ/CZ1-108[»]
4V8Yelectron microscopy4.30AZ1-108[»]
4V8Zelectron microscopy6.60AZ1-108[»]
4V92electron microscopy3.70Z42-105[»]
5DATX-ray3.15D5/d52-108[»]
5DC3X-ray3.25D5/d52-108[»]
5DGEX-ray3.45D5/d52-108[»]
5DGFX-ray3.30D5/d52-108[»]
5DGVX-ray3.10D5/d52-108[»]
5FCIX-ray3.40D5/d52-108[»]
5FCJX-ray3.10D5/d52-108[»]
5I4LX-ray3.10D5/d536-105[»]
5JUOelectron microscopy4.00WB1-108[»]
5JUPelectron microscopy3.50WB1-108[»]
5JUSelectron microscopy4.20WB1-108[»]
5JUTelectron microscopy4.00WB1-108[»]
5JUUelectron microscopy4.00WB1-108[»]
5LYBX-ray3.25D5/d536-105[»]
5M1Jelectron microscopy3.30Z236-105[»]
5MC6electron microscopy3.80K1-108[»]
5TGAX-ray3.30D5/d536-105[»]
5TGMX-ray3.50D5/d536-105[»]
ProteinModelPortaliQ3E792.
SMRiQ3E792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004856.
HOGENOMiHOG000203673.
InParanoidiQ3E792.
KOiK02975.
OMAiKEVPTFR.
OrthoDBiEOG092C5Z2K.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR004977. Ribosomal_S25.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR12850. PTHR12850. 1 hit.
PfamiView protein in Pfam
PF03297. Ribosomal_S25. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012268. Ribosomal_S25. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3E792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI
60 70 80 90 100
LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI

YTRATASE
Length:108
Mass (Da):12,039
Last modified:November 8, 2005 - v1
Checksum:iC52D0328DEA125B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16A → R AA sequence (PubMed:18782943).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti8S → F in strain: SK1. 1 Publication1
Natural varianti13A → T in strain: SK1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03013 Genomic DNA. Translation: CAA26797.1.
Z72812 Genomic DNA. Translation: CAA97010.1.
DQ115389 Genomic DNA. Translation: AAZ22490.1.
EF123137 mRNA. Translation: ABM97481.1.
BK006941 Genomic DNA. Translation: DAA08121.1.
PIRiS05844. R3BY31.
RefSeqiNP_011541.1. NM_001181156.1.

Genome annotation databases

EnsemblFungiiYGR027C; YGR027C; YGR027C.
GeneIDi852911.
KEGGisce:YGR027C.

Similar proteinsi

Entry informationi

Entry nameiRS25A_YEAST
AccessioniPrimary (citable) accession number: Q3E792
Secondary accession number(s): A2TBN4
, D6VUG0, P05758, P07282, Q45U56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 8, 2005
Last modified: September 27, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names