Q3E792 (RS25A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S25-A

Alternative name(s):
RP45
S31
YS23

Gene names

Name:	RPS25A
Synonyms:	RPS31A
Ordered Locus Names:	YGR027C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.8
Subcellular location	Cytoplasm Ref.9.
Miscellaneous	It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation. Present with 322000 molecules/cell in log phase SD medium. There are 2 genes for S25 in yeast.
Sequence similarities	Belongs to the ribosomal protein S25e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Methylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator PubMed 6814480. Source: SGD
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay PubMed 6814480. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay PubMed 6814480. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-16106,EBI-16106

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 108

107

40S ribosomal protein S25-A

PRO_0000043376

Amino acid modifications

Modified residue

2

1

N,N-dimethylproline; by NTM1 Ref.12

Modified residue

61

1

Phosphoserine Ref.11

Modified residue

63

1

Phosphoserine Ref.11

Natural variations

Natural variant

8

1

S → F in strain: SK1. Ref.6

Natural variant

13

1

A → T in strain: SK1. Ref.6

Experimental info

Sequence conflict

16

1

A → R AA sequence Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

108

Helix Strand Turn

Details...

Helix

45 – 53

9

Beta strand

58 – 61

4

Helix

62 – 67

6

Helix

75 – 83

9

Beta strand

84 – 88

5

Beta strand

90 – 93

4

Beta strand

100 – 102

3

Sequences

	Sequence		Length	Mass (Da)
	Q3E792 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: C52D0328DEA125B5	FASTA	108	12,039
	10 20 30 40 50 60 MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV 70 80 90 100 SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI YTRATASE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene for yeast ribosomal protein S31 contains an intron in the leader sequence." Nieuwint R.T.M., Molenaar C.M.T., van Bommel J.H., van Raamsdonk-Duin M.M.C., Mager W.H., Planta R.J. Curr. Genet. 10:1-5(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Carlsbergensis.
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-96. Strain: ATCC 201390 / BY4743.
[6]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-108, VARIANTS PHE-8 AND THR-13. Strain: SK1.
[7]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PROTEIN SEQUENCE OF 15-22.
[8]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61 AND SER-63, MASS SPECTROMETRY.
[12]	"Identification of protein N-terminal methyltransferases in yeast and humans." Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G. Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT PRO-2 BY NTM1/TAE1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03013 Genomic DNA. Translation: CAA26797.1.
Z72812 Genomic DNA. Translation: CAA97010.1.
DQ115389 Genomic DNA. Translation: AAZ22490.1.
EF123137 mRNA. Translation: ABM97481.1.
BK006941 Genomic DNA. Translation: DAA08121.1.

PIR

R3BY31. S05844.

RefSeq

NP_011541.1. NM_001181156.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZB	electron microscopy	-	V	1-108	[»]
3O2Z	X-ray	4.00	Q	1-108	[»]
3O30	X-ray	4.00	Q	1-108	[»]
3U5C	X-ray	3.00	Z	1-108	[»]
3U5G	X-ray	3.00	Z	1-108	[»]

ProteinModelPortal

Q3E792.

SMR

Q3E792. Positions 36-105.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q3E792. 6 interactions.

MINT

MINT-490524.

Proteomic databases

PaxDb

Q3E792.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR027C; YGR027C; YGR027C.

GeneID

852911.

KEGG

sce:YGR027C.

Organism-specific databases

CYGD

YGR027c.

SGD

S000003259. RPS25A.

Phylogenomic databases

eggNOG

COG4901.

GeneTree

ENSGT00390000004856.

HOGENOM

HOG000203673.

KO

K02975.

OMA

QLIYTRN.

OrthoDB

EOG4X0R34.

Gene expression databases

Genevestigator

Q3E792.

Family and domain databases

InterPro

IPR004977. Ribosomal_S25.
[Graphical view]

PANTHER

PTHR12850. PTHR12850. 1 hit.

Pfam

PF03297. Ribosomal_S25. 1 hit.
[Graphical view]

ProDom

PD012268. Ribosomal_S25. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

NextBio

972603.

Entry information

Entry name

RS25A_YEAST

Accession

Primary (citable) accession number: Q3E792
Secondary accession number(s): A2TBN4 , D6VUG0, P05758, P07282, Q45U56

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 8, 2005
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program