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Q3E792 (RS25A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S25-A
Alternative name(s):
RP45
S31
YS23
Gene names
Name:RPS25A
Synonyms:RPS31A
Ordered Locus Names:YGR027C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length108 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.8

Subcellular location

Cytoplasm Ref.9.

Miscellaneous

It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation.

Present with 322000 molecules/cell in log phase SD medium.

There are 2 genes for S25 in yeast.

Sequence similarities

Belongs to the ribosomal protein S25e family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-16106,EBI-16106

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 10810740S ribosomal protein S25-A
PRO_0000043376

Amino acid modifications

Modified residue21N,N-dimethylproline; by NTM1 Ref.11

Natural variations

Natural variant81S → F in strain: SK1. Ref.6
Natural variant131A → T in strain: SK1. Ref.6

Experimental info

Sequence conflict161A → R AA sequence Ref.7

Secondary structure

............. 108
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q3E792 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: C52D0328DEA125B5

FASTA10812,039
        10         20         30         40         50         60 
MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV 

        70         80         90        100 
SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI YTRATASE 

« Hide

References

« Hide 'large scale' references
[1]"The gene for yeast ribosomal protein S31 contains an intron in the leader sequence."
Nieuwint R.T.M., Molenaar C.M.T., van Bommel J.H., van Raamsdonk-Duin M.M.C., Mager W.H., Planta R.J.
Curr. Genet. 10:1-5(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Carlsbergensis.
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
Juneau K., Palm C., Miranda M., Davis R.W.
Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-96.
Strain: ATCC 201390 / BY4743.
[6]"Quantitative trait loci mapped to single-nucleotide resolution in yeast."
Deutschbauer A.M., Davis R.W.
Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-108, VARIANTS PHE-8 AND THR-13.
Strain: SK1.
[7]"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
Otaka E., Higo K., Itoh T.
Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE OF 15-22.
[8]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Identification of protein N-terminal methyltransferases in yeast and humans."
Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT PRO-2 BY NTM1/TAE1.
[12]"Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution."
Armache J.P., Jarasch A., Anger A.M., Villa E., Becker T., Bhushan S., Jossinet F., Habeck M., Dindar G., Franckenberg S., Marquez V., Mielke T., Thomm M., Berninghausen O., Beatrix B., Soding J., Westhof E., Wilson D.N., Beckmann R.
Proc. Natl. Acad. Sci. U.S.A. 107:19748-19753(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.80 ANGSTROMS).
[13]"Crystal structure of the eukaryotic ribosome."
Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.00 ANGSTROMS).
[14]"The structure of the eukaryotic ribosome at 3.0 A resolution."
Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G., Yusupov M.
Science 334:1524-1529(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03013 Genomic DNA. Translation: CAA26797.1.
Z72812 Genomic DNA. Translation: CAA97010.1.
DQ115389 Genomic DNA. Translation: AAZ22490.1.
EF123137 mRNA. Translation: ABM97481.1.
BK006941 Genomic DNA. Translation: DAA08121.1.
PIRR3BY31. S05844.
RefSeqNP_011541.1. NM_001181156.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IZBelectron microscopy-V1-108[»]
3O2ZX-ray4.00Q1-108[»]
3O30X-ray4.00Q1-108[»]
3U5CX-ray3.00Z1-108[»]
3U5GX-ray3.00Z1-108[»]
4BYLelectron microscopy4.30Z1-108[»]
4BYTelectron microscopy6.60Z1-108[»]
ProteinModelPortalQ3E792.
SMRQ3E792. Positions 36-105.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33268. 81 interactions.
IntActQ3E792. 8 interactions.
MINTMINT-490524.

Proteomic databases

PaxDbQ3E792.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR027C; YGR027C; YGR027C.
GeneID852911.
KEGGsce:YGR027C.

Organism-specific databases

CYGDYGR027c.
SGDS000003259. RPS25A.

Phylogenomic databases

eggNOGCOG4901.
GeneTreeENSGT00390000004856.
HOGENOMHOG000203673.
KOK02975.
OMAQLIYTRS.
OrthoDBEOG7647G6.

Enzyme and pathway databases

BioCycYEAST:G3O-30751-MONOMER.

Gene expression databases

GenevestigatorQ3E792.

Family and domain databases

InterProIPR004977. Ribosomal_S25.
[Graphical view]
PANTHERPTHR12850. PTHR12850. 1 hit.
PfamPF03297. Ribosomal_S25. 1 hit.
[Graphical view]
ProDomPD012268. Ribosomal_S25. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

NextBio972603.

Entry information

Entry nameRS25A_YEAST
AccessionPrimary (citable) accession number: Q3E792
Secondary accession number(s): A2TBN4 expand/collapse secondary AC list , D6VUG0, P05758, P07282, Q45U56
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 8, 2005
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references