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Q3E792

- RS25A_YEAST

UniProt

Q3E792 - RS25A_YEAST

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Protein

40S ribosomal protein S25-A

Gene
RPS25A, RPS31A, YGR027C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30751-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S25-A
Alternative name(s):
RP45
S31
YS23
Gene namesi
Name:RPS25A
Synonyms:RPS31A
Ordered Locus Names:YGR027C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR027c.
SGDiS000003259. RPS25A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 10810740S ribosomal protein S25-APRO_0000043376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N,N-dimethylproline; by NTM11 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiQ3E792.
PaxDbiQ3E792.

Expressioni

Gene expression databases

GenevestigatoriQ3E792.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-16106,EBI-16106

Protein-protein interaction databases

BioGridi33268. 81 interactions.
IntActiQ3E792. 8 interactions.
MINTiMINT-490524.

Structurei

Secondary structure

1
108
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi45 – 539
Beta strandi58 – 614
Helixi62 – 676
Helixi75 – 839
Beta strandi84 – 885
Beta strandi90 – 934
Beta strandi100 – 1023

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW9electron microscopy6.10a1-108[»]
1VWVelectron microscopy6.10a1-108[»]
3IZBelectron microscopy-V1-108[»]
3O2ZX-ray4.00Q1-108[»]
3O30X-ray4.00Q1-108[»]
3U5CX-ray3.00Z1-108[»]
3U5GX-ray3.00Z1-108[»]
4BYLelectron microscopy4.30Z1-108[»]
4BYTelectron microscopy6.60Z1-108[»]
4CUYelectron microscopy3.70Z42-105[»]
ProteinModelPortaliQ3E792.
SMRiQ3E792. Positions 36-105.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4901.
GeneTreeiENSGT00390000004856.
HOGENOMiHOG000203673.
KOiK02975.
OMAiQLIYTRS.
OrthoDBiEOG7647G6.

Family and domain databases

InterProiIPR004977. Ribosomal_S25.
[Graphical view]
PANTHERiPTHR12850. PTHR12850. 1 hit.
PfamiPF03297. Ribosomal_S25. 1 hit.
[Graphical view]
ProDomiPD012268. Ribosomal_S25. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3E792-1 [UniParc]FASTAAdd to Basket

« Hide

MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI    50
LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGIIK PISKHSKQAI 100
YTRATASE 108
Length:108
Mass (Da):12,039
Last modified:November 8, 2005 - v1
Checksum:iC52D0328DEA125B5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81S → F in strain: SK1. 1 Publication
Natural varianti13 – 131A → T in strain: SK1. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161A → R AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03013 Genomic DNA. Translation: CAA26797.1.
Z72812 Genomic DNA. Translation: CAA97010.1.
DQ115389 Genomic DNA. Translation: AAZ22490.1.
EF123137 mRNA. Translation: ABM97481.1.
BK006941 Genomic DNA. Translation: DAA08121.1.
PIRiS05844. R3BY31.
RefSeqiNP_011541.1. NM_001181156.1.

Genome annotation databases

EnsemblFungiiYGR027C; YGR027C; YGR027C.
GeneIDi852911.
KEGGisce:YGR027C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03013 Genomic DNA. Translation: CAA26797.1 .
Z72812 Genomic DNA. Translation: CAA97010.1 .
DQ115389 Genomic DNA. Translation: AAZ22490.1 .
EF123137 mRNA. Translation: ABM97481.1 .
BK006941 Genomic DNA. Translation: DAA08121.1 .
PIRi S05844. R3BY31.
RefSeqi NP_011541.1. NM_001181156.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VW9 electron microscopy 6.10 a 1-108 [» ]
1VWV electron microscopy 6.10 a 1-108 [» ]
3IZB electron microscopy - V 1-108 [» ]
3O2Z X-ray 4.00 Q 1-108 [» ]
3O30 X-ray 4.00 Q 1-108 [» ]
3U5C X-ray 3.00 Z 1-108 [» ]
3U5G X-ray 3.00 Z 1-108 [» ]
4BYL electron microscopy 4.30 Z 1-108 [» ]
4BYT electron microscopy 6.60 Z 1-108 [» ]
4CUY electron microscopy 3.70 Z 42-105 [» ]
ProteinModelPortali Q3E792.
SMRi Q3E792. Positions 36-105.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33268. 81 interactions.
IntActi Q3E792. 8 interactions.
MINTi MINT-490524.

Proteomic databases

MaxQBi Q3E792.
PaxDbi Q3E792.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR027C ; YGR027C ; YGR027C .
GeneIDi 852911.
KEGGi sce:YGR027C.

Organism-specific databases

CYGDi YGR027c.
SGDi S000003259. RPS25A.

Phylogenomic databases

eggNOGi COG4901.
GeneTreei ENSGT00390000004856.
HOGENOMi HOG000203673.
KOi K02975.
OMAi QLIYTRS.
OrthoDBi EOG7647G6.

Enzyme and pathway databases

BioCyci YEAST:G3O-30751-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

NextBioi 972603.

Gene expression databases

Genevestigatori Q3E792.

Family and domain databases

InterProi IPR004977. Ribosomal_S25.
[Graphical view ]
PANTHERi PTHR12850. PTHR12850. 1 hit.
Pfami PF03297. Ribosomal_S25. 1 hit.
[Graphical view ]
ProDomi PD012268. Ribosomal_S25. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene for yeast ribosomal protein S31 contains an intron in the leader sequence."
    Nieuwint R.T.M., Molenaar C.M.T., van Bommel J.H., van Raamsdonk-Duin M.M.C., Mager W.H., Planta R.J.
    Curr. Genet. 10:1-5(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Carlsbergensis.
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-96.
    Strain: ATCC 201390 / BY4743.
  6. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-108, VARIANTS PHE-8 AND THR-13.
    Strain: SK1.
  7. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 15-22.
  8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Identification of protein N-terminal methyltransferases in yeast and humans."
    Webb K.J., Lipson R.S., Al-Hadid Q., Whitelegge J.P., Clarke S.G.
    Biochemistry 49:5225-5235(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT PRO-2 BY NTM1/TAE1.
  12. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.80 ANGSTROMS).
  13. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.00 ANGSTROMS).
  14. Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).

Entry informationi

Entry nameiRS25A_YEAST
AccessioniPrimary (citable) accession number: Q3E792
Secondary accession number(s): A2TBN4
, D6VUG0, P05758, P07282, Q45U56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 8, 2005
Last modified: September 3, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

It is presumed that the precursor part of S25 is engaged in assembling of the small subunit, thus being essential in ribosome maturation.
Present with 322000 molecules/cell in log phase SD medium.
There are 2 genes for S25 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi