Q3E754 (RS21B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S21-B

Alternative name(s):
S26
YS25

Gene names

Name:	RPS21B
Synonyms:	RPS25B, RPS26B
Ordered Locus Names:	YJL136C
ORF Names:	J0664

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability. Ref.8
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.6 Ref.8.
Post-translational modification	N-terminally acetylated by acetyltransferase NatB.
Disruption phenotype	Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size. Ref.8
Miscellaneous	Present with 15300 molecules/cell in log phase SD medium. There are 2 genes for S21 in yeast.
Sequence similarities	Belongs to the ribosomal protein S21e family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator PubMed 3533916. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.8. Source: SGD endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.8. Source: SGD translational elongation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay PubMed 3533916. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay PubMed 3533916. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 87	87	40S ribosomal protein S21-B		PRO_0000194762
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.5
	Modified residue	65	1	Phosphoserine Ref.10 Ref.11
	Modified residue	68	1	Phosphoserine Ref.11
	Modified residue	84	1	Phosphoserine Ref.9 Ref.11
	Modified residue	86	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	Q3E754 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 52E1C247575A7410	FASTA	87	9,760
	10 20 30 40 50 60 MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY ITYALSGYVR 70 80 SRGESDDSLN RLAQNDGLLK NVWSYSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT MET-1 BY NATB.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA." Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R. Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-68; SER-84 AND SER-86, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87371 Genomic DNA. Translation: CAA60819.1. Z49411 Genomic DNA. Translation: CAA89431.1. BK006943 Genomic DNA. Translation: DAA08665.1.
PIR	S56918.
RefSeq	NP_012399.1. NM_001181569.1.
3D structure databases
ProteinModelPortal	Q3E754.
SMR	Q3E754. Positions 1-87.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3E754. 1 interaction.
Proteomic databases
PaxDb	Q3E754.
PeptideAtlas	Q3E754.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL136C; YJL136C; YJL136C.
GeneID	853305.
KEGG	sce:YJL136C.
Organism-specific databases
CYGD	YJL136c.
SGD	S000003672. RPS21B.
Phylogenomic databases
eggNOG	NOG301320.
GeneTree	ENSGT00390000017515.
HOGENOM	HOG000183557.
KO	K02971.
OMA	ICGAIRI.
OrthoDB	EOG4S7P0Q.
Gene expression databases
Genevestigator	Q3E754.
Family and domain databases
InterPro	IPR001931. Ribosomal_S21e. IPR018279. Ribosomal_S21e_CS. [Graphical view]
PANTHER	PTHR10442. PTHR10442. 1 hit.
Pfam	PF01249. Ribosomal_S21e. 1 hit. [Graphical view]
PIRSF	PIRSF002148. Ribosomal_S21e. 1 hit.
ProDom	PD006584. Ribosomal_S21e. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00996. RIBOSOMAL_S21E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973633.

Entry information

Entry name

RS21B_YEAST

Accession

Primary (citable) accession number: Q3E754
Secondary accession number(s): D6VW49, P05760

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	November 8, 2005
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program