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Protein

Sprouty-related, EVH1 domain-containing protein 2

Gene

Spred2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.By similarity

GO - Molecular functioni

  1. protein serine/threonine kinase inhibitor activity Source: Ensembl

GO - Biological processi

  1. inactivation of MAPK activity Source: Ensembl
  2. multicellular organismal development Source: InterPro
  3. negative regulation of peptidyl-threonine phosphorylation Source: Ensembl
  4. positive regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  5. regulation of protein deacetylation Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Sprouty-related, EVH1 domain-containing protein 2
Short name:
Spred-2
Gene namesi
Name:Spred2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 14

Organism-specific databases

RGDi1309304. Spred2.

Subcellular locationi

Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesiclesecretory vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated. Cytoplasm
Note: Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.By similarity

GO - Cellular componenti

  1. nucleus Source: Ensembl
  2. plasma membrane Source: Ensembl
  3. transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Sprouty-related, EVH1 domain-containing protein 2PRO_0000354679Add
BLAST

Post-translational modificationi

Phosphorylated on tyrosine. Phosphorylation of Tyr-224 and Tyr-227 are required for ubiquitination (By similarity).By similarity
Ubiquitinated; leading to degradation by the proteasome.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3C2P8.
PRIDEiQ3C2P8.

PTM databases

PhosphoSiteiQ3C2P8.

Expressioni

Gene expression databases

GenevestigatoriQ3C2P8.

Interactioni

Subunit structurei

Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053806.

Structurei

3D structure databases

ProteinModelPortaliQ3C2P8.
SMRiQ3C2P8. Positions 1-124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 122118WH1PROSITE-ProRule annotationAdd
BLAST
Domaini197 – 25256KBDPROSITE-ProRule annotationAdd
BLAST
Domaini300 – 408109SPRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 KBD domain.PROSITE-ProRule annotation
Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG277322.
GeneTreeiENSGT00730000110272.
HOGENOMiHOG000220886.
HOVERGENiHBG057556.
InParanoidiQ3C2P8.
KOiK04703.
OMAiDHYHLEQ.
OrthoDBiEOG7T7GV7.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR023337. KBD.
IPR011993. PH_like_dom.
IPR007875. Sprouty.
IPR000697. WH1/EVH1.
[Graphical view]
PfamiPF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
PROSITEiPS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3C2P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEETHPDDD SYIVRVKAVV MTRDDSSGGW FPQEGGGISR VGVCKVMHPE
60 70 80 90 100
GNGRSGFLIH GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL
110 120 130 140 150
TFQSPADARA FDRGVRKAIE DLIEGSTTSS STIHNEAELG DDDVFTTATD
160 170 180 190 200
SSSNSSQKRE PNTRTISSPT SCEHRRIYTL DPYPMDLYHP DQRLPRSYPQ
210 220 230 240 250
VTFPEDDEEI VRINPREKIW MTGYEDYRHA PVRGKYLDST EDADSYVRFA
260 270 280 290 300
KGEVPKHEYT YPYVDSSDFG FGEDPKGNVI KTQPPRAKSR RRKENGERSR
310 320 330 340 350
CVYCRDMFNH EENRRGHCQD APDAVRTCIR RVSCMWCADS MLYHCMSDPE
360 370 380 390 400
GDYTDPCSCD TSDEKFCLRW MALIALSFLA PCMCCYLPLR ACHHCGVMCR
410
CCGGKHKAAA
Length:410
Mass (Da):46,805
Last modified:November 22, 2005 - v1
Checksum:i18F2F3FBB616BCBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125136 mRNA. Translation: BAE46587.1.
RefSeqiNP_001040559.1. NM_001047094.1.
UniGeneiRn.225245.

Genome annotation databases

EnsembliENSRNOT00000006573; ENSRNOP00000006573; ENSRNOG00000004888.
GeneIDi305539.
KEGGirno:305539.
UCSCiRGD:1309304. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125136 mRNA. Translation: BAE46587.1.
RefSeqiNP_001040559.1. NM_001047094.1.
UniGeneiRn.225245.

3D structure databases

ProteinModelPortaliQ3C2P8.
SMRiQ3C2P8. Positions 1-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053806.

PTM databases

PhosphoSiteiQ3C2P8.

Proteomic databases

PaxDbiQ3C2P8.
PRIDEiQ3C2P8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006573; ENSRNOP00000006573; ENSRNOG00000004888.
GeneIDi305539.
KEGGirno:305539.
UCSCiRGD:1309304. rat.

Organism-specific databases

CTDi200734.
RGDi1309304. Spred2.

Phylogenomic databases

eggNOGiNOG277322.
GeneTreeiENSGT00730000110272.
HOGENOMiHOG000220886.
HOVERGENiHBG057556.
InParanoidiQ3C2P8.
KOiK04703.
OMAiDHYHLEQ.
OrthoDBiEOG7T7GV7.

Miscellaneous databases

NextBioi654751.

Gene expression databases

GenevestigatoriQ3C2P8.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR023337. KBD.
IPR011993. PH_like_dom.
IPR007875. Sprouty.
IPR000697. WH1/EVH1.
[Graphical view]
PfamiPF05210. Sprouty. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
PROSITEiPS51488. KBD. 1 hit.
PS51227. SPR. 1 hit.
PS50229. WH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of cDNA encoding rat Spred-2."
    Saitoh A., Hirasawa N., Ohuchi K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiSPRE2_RAT
AccessioniPrimary (citable) accession number: Q3C2P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 22, 2005
Last modified: March 4, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.